식용식물자원으로부터 활성물질의 탐색-IX. 흰씀바귀(Ixeris dentata forma albiflora)뿌리에서 Sesquiterpene Lactone 화합물의 분리 및 구조 동정; ACAT, DGAT 및 FPTase 효소 활성의 저해 Screening of Biologically Active Compound from Edible Plant Sources-IX. Isolation and Identification ofSesquiterpene Lactons Isolated from the Root of Ixeris dentata forma albiflora;
Applied Biological Chemistry
v.47
no.2
2004년, pp.251 - 257
The root of Ixeris dentata forma albiflora was extracted with 80% aqueous MeOH and solventfractionated with EtOAc, n-BuOH and water, successively. From the EtOAc and n-BuOH fractions, foursesquiterpene compounds were isolated through the repeated silica gel and ODS column chromatographies. Thechemical structures were determined as zaluzanin C (1), 9α-hydroxyguaian-4(15),10(14),11(13)-triene- 6,12-olide (2),3β-O-β-D-glucopyranosyl-8α-hydroxyguaian-4(15),10(14)-diene-6,12-olide (3), and 3β-O-β-D-glucopyranosyl-8β-hydroxyguaian-10(14)-ene-6,12-olide (4) through the interpretation of several spectral data including 2D-NMR. Someshowed the inhibitory effects on DGAT (Diacylglycerol acyltransferase), (IC50 values of 1, 2: 0.13, 0.10 mM), thecatalyzing enzymes of the intracellular esterification of diacylglycerol and FPTase (Farnesyl-protein transferase), (IC50values of 1, 2: 0.15, 0.18 mM), the farnesylation enzyme for Ras protein charge of cancer promotion.
The root of Ixeris dentata forma albiflora was extracted with 80% aqueous MeOH and solventfractionated with EtOAc, n-BuOH and water, successively. From the EtOAc and n-BuOH fractions, foursesquiterpene compounds were isolated through the repeated silica gel and ODS column chromatographies. Thechemical structures were determined as zaluzanin C (1), 9α-hydroxyguaian-4(15),10(14),11(13)-triene- 6,12-olide (2),3β-O-β-D-glucopyranosyl-8α-hydroxyguaian-4(15),10(14)-diene-6,12-olide (3), and 3β-O-β-D-glucopyranosyl-8β-hydroxyguaian-10(14)-ene-6,12-olide (4) through the interpretation of several spectral data including 2D-NMR. Someshowed the inhibitory effects on DGAT (Diacylglycerol acyltransferase), (IC50 values of 1, 2: 0.13, 0.10 mM), thecatalyzing enzymes of the intracellular esterification of diacylglycerol and FPTase (Farnesyl-protein transferase), (IC50values of 1, 2: 0.15, 0.18 mM), the farnesylation enzyme for Ras protein charge of cancer promotion.
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