통계학적 실험계획법을 이용한 미생물유래 Transglutaminase 생산을 위한 배지최적화와 회분식배양 Medium Optimizaion and Batch Culture for Microbial Transglutaminase Production by statistical Experimantal Design원문보기
Streptomyces mobaraensis에 의한 미생물유래 transglutaminase 생산을 위한 배지최적화와 발효조건을 통계학적 실험계획법을 이용하여 조사하였다. 성장배지의 탄소원의 종류와 농도에 따른 미생물의 성장곡선을 조사함으로써 성장배지 조성을 확립할 수 있었으며, 확립된 배지조성(g/L)은 soluble starch 30, proteose peptone 20, K_(2)HPO_(4) 2와 MgSO_(4) 1이다. 미생물 배양 시 성장배지에서 생산배지에 접종하는 시기 및 접종량 등은 균체의 형태구조 및 ...
Streptomyces mobaraensis에 의한 미생물유래 transglutaminase 생산을 위한 배지최적화와 발효조건을 통계학적 실험계획법을 이용하여 조사하였다. 성장배지의 탄소원의 종류와 농도에 따른 미생물의 성장곡선을 조사함으로써 성장배지 조성을 확립할 수 있었으며, 확립된 배지조성(g/L)은 soluble starch 30, proteose peptone 20, K_(2)HPO_(4) 2와 MgSO_(4) 1이다. 미생물 배양 시 성장배지에서 생산배지에 접종하는 시기 및 접종량 등은 균체의 형태구조 및 이차대사산물의 생산량과 밀접한 관계가 있다. 발효중 종균의 접종시기와 접종량이 미생물유래 transglutaminase 생산에 미치는 영향을 구명하였다. 미생물유래 transglutaminase 생산을 위한 최적의 접종시기와 접종량은 성장배지에 24시간 배양한 후 5% 접종 시였다. Plackett-Burman design을 이용하여 효소생산에 있어 가장 많은 영향을 미치는 배지성분을 조사한 결과 soluble starch, proteose peptone, yeast extract로 선정되었다. 이를 이용하여 배지최적화를 하기위해 중심합성계획과 반응표면분석법을 이용하였다. 반응표면분석을 이용한 배지 최적화 실험을 통하여 확립된 배지조성(g/L)은 soluble starch 48, proteose peptone 15, yeast extract 4, NaH_(2)PO_(4)·_(2)H_(2)O 1, MgSO_(4) 1, Urea 2와 PPG(polypropyleneglycol) 0.5이었으며, 미생물유래 transglutaminase 생산은 2.55 U/mL로 기본 생산배지에 비해 1.6배 향상되었다. 반응표면 분석을 통해 얻어진 결과를 토대로 생물반응기에 적용하여 회분식 배양을 수행한 결과 미생물유래 transglutaminase 생산은 2.80 U/ml로 기본 생산배지에 비해 1.8배 향상되었다. 유기질소원인 proteose peptone(15 g/L)을 유가식 배양기간에 3일간 연속적으로 공급하여 수행한 결과 미생물유래 transglutaminase 생산은 2.90 U/mL로 회분식 배양에 비해 약 1.1배 향상되었다.
Streptomyces mobaraensis에 의한 미생물유래 transglutaminase 생산을 위한 배지최적화와 발효조건을 통계학적 실험계획법을 이용하여 조사하였다. 성장배지의 탄소원의 종류와 농도에 따른 미생물의 성장곡선을 조사함으로써 성장배지 조성을 확립할 수 있었으며, 확립된 배지조성(g/L)은 soluble starch 30, proteose peptone 20, K_(2)HPO_(4) 2와 MgSO_(4) 1이다. 미생물 배양 시 성장배지에서 생산배지에 접종하는 시기 및 접종량 등은 균체의 형태구조 및 이차대사산물의 생산량과 밀접한 관계가 있다. 발효중 종균의 접종시기와 접종량이 미생물유래 transglutaminase 생산에 미치는 영향을 구명하였다. 미생물유래 transglutaminase 생산을 위한 최적의 접종시기와 접종량은 성장배지에 24시간 배양한 후 5% 접종 시였다. Plackett-Burman design을 이용하여 효소생산에 있어 가장 많은 영향을 미치는 배지성분을 조사한 결과 soluble starch, proteose peptone, yeast extract로 선정되었다. 이를 이용하여 배지최적화를 하기위해 중심합성계획과 반응표면분석법을 이용하였다. 반응표면분석을 이용한 배지 최적화 실험을 통하여 확립된 배지조성(g/L)은 soluble starch 48, proteose peptone 15, yeast extract 4, NaH_(2)PO_(4)·_(2)H_(2)O 1, MgSO_(4) 1, Urea 2와 PPG(polypropyleneglycol) 0.5이었으며, 미생물유래 transglutaminase 생산은 2.55 U/mL로 기본 생산배지에 비해 1.6배 향상되었다. 반응표면 분석을 통해 얻어진 결과를 토대로 생물반응기에 적용하여 회분식 배양을 수행한 결과 미생물유래 transglutaminase 생산은 2.80 U/ml로 기본 생산배지에 비해 1.8배 향상되었다. 유기질소원인 proteose peptone(15 g/L)을 유가식 배양기간에 3일간 연속적으로 공급하여 수행한 결과 미생물유래 transglutaminase 생산은 2.90 U/mL로 회분식 배양에 비해 약 1.1배 향상되었다.
Statistical experimental design were used to determine optimum levels of medium nutrients and fermentation conditions for the transglutaminase production by Streptomyces mobaraensis. In the previous study, we were investigated growth medium components. The effect of various carbon source concentrati...
Statistical experimental design were used to determine optimum levels of medium nutrients and fermentation conditions for the transglutaminase production by Streptomyces mobaraensis. In the previous study, we were investigated growth medium components. The effect of various carbon source concentration was tested with glucose and starch on the mTG production. The optimized growth medium composition found consisted of 30 g/L soluble starch, 20 g/L proteose peptone, 2 g/L K_(2)HPO_(4) and 1 g/L MgSO_(4). It is known that inoculum age and size is related deeply morphology and secondary metabolism. The effect of inoculum age and size in morphology and microbial transglutaminase production was examined. The optimum inoculum age and size was 24 hours and 5% respectively for the enzyme productoin. Information about the effects of different medium components on mTG production were investigated by using the Plackett-Burman design. Soluble starch, proteose peptone and yeast extract were found to significantly influence the mTG production. The various factors of medium components (soluble starch, proteose peptone, yeast extract) were tested to find the optimal medium components for maximum mTG production by using the central composite design and response surface methodilogy. As a results, of the above statements analyzed by statistical analysis system(SAS) program. The optimized medium composition found consisted of 48 g/L soluble starch, 15 g/L proteose peptone, 4 g/L yeast extract, 1 g/L NaH_(2)PO_(4)·2H_(2)O, 1 g/L MgSO_(4), 2 g/L Urea and 0.5 g/L PPG. The mTG production was 2.55 U/mL, which was found to be improved almost 1.6 times in comparison with that in the basic production medium in shake flask fermentation. In bioreactor, fermentation by using optimized medium obtained from response surface methodology were performed during a batch culture. The mTG production was 2.80 U/mL, and it was improved about 1.8 times in comparison with that for the basic production medium in shake flasks. In fed-batch culture, proteose peptone(15 g/L) was supplied continuously for 3 days. As a result, the production of microbial transglutaminase were 2.90 U/mL, which was found to be improved almost 1.1 times in comparison with that in batch culture.
Statistical experimental design were used to determine optimum levels of medium nutrients and fermentation conditions for the transglutaminase production by Streptomyces mobaraensis. In the previous study, we were investigated growth medium components. The effect of various carbon source concentration was tested with glucose and starch on the mTG production. The optimized growth medium composition found consisted of 30 g/L soluble starch, 20 g/L proteose peptone, 2 g/L K_(2)HPO_(4) and 1 g/L MgSO_(4). It is known that inoculum age and size is related deeply morphology and secondary metabolism. The effect of inoculum age and size in morphology and microbial transglutaminase production was examined. The optimum inoculum age and size was 24 hours and 5% respectively for the enzyme productoin. Information about the effects of different medium components on mTG production were investigated by using the Plackett-Burman design. Soluble starch, proteose peptone and yeast extract were found to significantly influence the mTG production. The various factors of medium components (soluble starch, proteose peptone, yeast extract) were tested to find the optimal medium components for maximum mTG production by using the central composite design and response surface methodilogy. As a results, of the above statements analyzed by statistical analysis system(SAS) program. The optimized medium composition found consisted of 48 g/L soluble starch, 15 g/L proteose peptone, 4 g/L yeast extract, 1 g/L NaH_(2)PO_(4)·2H_(2)O, 1 g/L MgSO_(4), 2 g/L Urea and 0.5 g/L PPG. The mTG production was 2.55 U/mL, which was found to be improved almost 1.6 times in comparison with that in the basic production medium in shake flask fermentation. In bioreactor, fermentation by using optimized medium obtained from response surface methodology were performed during a batch culture. The mTG production was 2.80 U/mL, and it was improved about 1.8 times in comparison with that for the basic production medium in shake flasks. In fed-batch culture, proteose peptone(15 g/L) was supplied continuously for 3 days. As a result, the production of microbial transglutaminase were 2.90 U/mL, which was found to be improved almost 1.1 times in comparison with that in batch culture.
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