Bilberry(Vaccinium myrtillus L.)와 bilberry(Vaccinium myrtillus L.) solution, blueberry(Vaccinium corymbosum L.)의 total methanol(MeOH) extract를 ethyl acetate(EtOAc), water(H2O)로 분획하여 총 폴리페놀 측정, DPPH 라디칼 소거작용, ...
Bilberry(Vaccinium myrtillus L.)와 bilberry(Vaccinium myrtillus L.) solution, blueberry(Vaccinium corymbosum L.)의 total methanol(MeOH) extract를 ethyl acetate(EtOAc), water(H2O)로 분획하여 총 폴리페놀 측정, DPPH 라디칼 소거작용, nitrite 소거작용, ABTS 라디칼 저해작용과 SOD 유사활성을 측정하였다. 모든 분획물이 농도 의존적으로 유의성 있게 활성을 가짐을 확인하였고 그 중 EtOAc fraction의 항산화능이 전반적으로 가장 강하였으며 total MeOH extract, water fraction 순으로 작용이 강하였다. 총 폴리페놀(total polyphenol) 양의 측정결과, EtOAc fraction에서 bilberry solution과 bilberry가 blueberry보다도 그 함량이 높았다(11.67 mg/g GAE (gallic acid equivalent), 9.50 mg/g GAE , 4.59 mg/g GAE). DPPH 라디칼 소거 작용 실험에서는 bilberry와 blueberry, bilberry solution의 EtOAc fraction의 활성이 전반적으로 유사하게 높아 125 ~ 250 ppm에서 이미 최대 활성을 보였다(84.00 %, 75.76 %, 78.63 %). Nitrite 소거 작용 실험에서는 bilberry와 bilberry solution의 EtOAc fraction이 62.5 ppm에서 각각 91.23 %와 91.15 %의 최대 활성을 보였으며, blueberry의 EtOAc fraction도 125 ppm에서 90.21 % 소거 활성을 보임으로써 매우 낮은 농도에서도 모두 활성이 컸다. ABTS 라디칼 저해 작용 실험에서는 bilberry와 blueberry의 EtOAc fraction이 62.5 ppm에서 각각 90.41 %와 86.44 %의 활성을 보였고, bilberry solution의 EtOAc fraction은 125 ppm에서 89.81 %의 강한 활성을 보였다. SOD 유사 활성은 위의 항산화 활성보다는 가장 약하여 bilberry와 bilberry solution, blueberry의 EtOAc fraction이 1000 ppm에서 최대 활성을 보였으나(각각 95.58 %, 84.99 %, 83.54 %), 그 EC50값은 항산화 활성이 알려진 chlorogenic acid의 EC50값 453 ppm보다는 활성이 높아 그 EC50값이 각각 250 ppm, 251 ppm, 481 ppm이었다. 위의 여러 항산화 실험의 결과 bilberry와 blueberry의 항산화 활성은 현격한 차이가 있음을 알 수 있었으며, bilberry solution은 여러 작용탐색으로 비교한 결과 bilberry와 유사한 경향이 증명되었다.
Bilberry(Vaccinium myrtillus L.)와 bilberry(Vaccinium myrtillus L.) solution, blueberry(Vaccinium corymbosum L.)의 total methanol(MeOH) extract를 ethyl acetate(EtOAc), water(H2O)로 분획하여 총 폴리페놀 측정, DPPH 라디칼 소거작용, nitrite 소거작용, ABTS 라디칼 저해작용과 SOD 유사활성을 측정하였다. 모든 분획물이 농도 의존적으로 유의성 있게 활성을 가짐을 확인하였고 그 중 EtOAc fraction의 항산화능이 전반적으로 가장 강하였으며 total MeOH extract, water fraction 순으로 작용이 강하였다. 총 폴리페놀(total polyphenol) 양의 측정결과, EtOAc fraction에서 bilberry solution과 bilberry가 blueberry보다도 그 함량이 높았다(11.67 mg/g GAE (gallic acid equivalent), 9.50 mg/g GAE , 4.59 mg/g GAE). DPPH 라디칼 소거 작용 실험에서는 bilberry와 blueberry, bilberry solution의 EtOAc fraction의 활성이 전반적으로 유사하게 높아 125 ~ 250 ppm에서 이미 최대 활성을 보였다(84.00 %, 75.76 %, 78.63 %). Nitrite 소거 작용 실험에서는 bilberry와 bilberry solution의 EtOAc fraction이 62.5 ppm에서 각각 91.23 %와 91.15 %의 최대 활성을 보였으며, blueberry의 EtOAc fraction도 125 ppm에서 90.21 % 소거 활성을 보임으로써 매우 낮은 농도에서도 모두 활성이 컸다. ABTS 라디칼 저해 작용 실험에서는 bilberry와 blueberry의 EtOAc fraction이 62.5 ppm에서 각각 90.41 %와 86.44 %의 활성을 보였고, bilberry solution의 EtOAc fraction은 125 ppm에서 89.81 %의 강한 활성을 보였다. SOD 유사 활성은 위의 항산화 활성보다는 가장 약하여 bilberry와 bilberry solution, blueberry의 EtOAc fraction이 1000 ppm에서 최대 활성을 보였으나(각각 95.58 %, 84.99 %, 83.54 %), 그 EC50값은 항산화 활성이 알려진 chlorogenic acid의 EC50값 453 ppm보다는 활성이 높아 그 EC50값이 각각 250 ppm, 251 ppm, 481 ppm이었다. 위의 여러 항산화 실험의 결과 bilberry와 blueberry의 항산화 활성은 현격한 차이가 있음을 알 수 있었으며, bilberry solution은 여러 작용탐색으로 비교한 결과 bilberry와 유사한 경향이 증명되었다.
Simultaneous comparison of bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L.) fruits for their antioxidant activities were reported. Which fraction would be most active after fractionation and whether the commercially available bilberry solution originated from bilberry fruit ...
Simultaneous comparison of bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L.) fruits for their antioxidant activities were reported. Which fraction would be most active after fractionation and whether the commercially available bilberry solution originated from bilberry fruit were also studied. From the total methanol extract of bilberry, bilberry solution and blueberry, ethyl acetate (EtOAc) and water fraction were made from this extract and various antioxidant characteristics of these were identified in terms of total polyphenol contents (TPC) , 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, nitrite scavenging ability, 2,2'-azinobis (3-ethylbenzo thizoline-6-sulfonic acid) (ABTS) radical cation inhibition assay and superoxide dismutase (SOD) -like activity. Activities of all of the samples were increased dose-dependently and the EtOAc fraction of all samples showed most potent antioxidant activities. The total polyphenol contents in the EtOAc fractions of bilberry solution and bilberry were found to be 11.67 mg/g gallic acid equivalent (GAE) and 9.50 mg/g GAE, respectively and that of blueberry was very small as 4.59 mg/g GAE. The IC50 values of DPPH scavenging activities in the EtOAc fractions of bilberry, blueberry and bilberry solution were 27 ppm, 34 ppm and 48 ppm, respectively and that of standard ascorbic acid was 20 ppm in the same system. The nitrite scavenging abilities was most potent and the IC50 values of the EtOAc fractions in all of the samples showed the similar results (19 ppm, 23 ppm, 29 ppm) to that of standard ascorbic acid, 13 ppm. As for ABTS radical cation inhibition assay, the IC50 values in EtOAc fractions of blueberry and bilberry were nearly same as 26 ppm and 28 ppm, respectively, compared with that of ascorbic acid, 12 ppm. The SOD-like activities were slightly low in all samples. In these results, the EtOAc fraction of bilberry was generally most excellent in all of the other fractions and the nitrite scavenging ability was found to be evidently most active in all of the other antioxidant systems.
Simultaneous comparison of bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L.) fruits for their antioxidant activities were reported. Which fraction would be most active after fractionation and whether the commercially available bilberry solution originated from bilberry fruit were also studied. From the total methanol extract of bilberry, bilberry solution and blueberry, ethyl acetate (EtOAc) and water fraction were made from this extract and various antioxidant characteristics of these were identified in terms of total polyphenol contents (TPC) , 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, nitrite scavenging ability, 2,2'-azinobis (3-ethylbenzo thizoline-6-sulfonic acid) (ABTS) radical cation inhibition assay and superoxide dismutase (SOD) -like activity. Activities of all of the samples were increased dose-dependently and the EtOAc fraction of all samples showed most potent antioxidant activities. The total polyphenol contents in the EtOAc fractions of bilberry solution and bilberry were found to be 11.67 mg/g gallic acid equivalent (GAE) and 9.50 mg/g GAE, respectively and that of blueberry was very small as 4.59 mg/g GAE. The IC50 values of DPPH scavenging activities in the EtOAc fractions of bilberry, blueberry and bilberry solution were 27 ppm, 34 ppm and 48 ppm, respectively and that of standard ascorbic acid was 20 ppm in the same system. The nitrite scavenging abilities was most potent and the IC50 values of the EtOAc fractions in all of the samples showed the similar results (19 ppm, 23 ppm, 29 ppm) to that of standard ascorbic acid, 13 ppm. As for ABTS radical cation inhibition assay, the IC50 values in EtOAc fractions of blueberry and bilberry were nearly same as 26 ppm and 28 ppm, respectively, compared with that of ascorbic acid, 12 ppm. The SOD-like activities were slightly low in all samples. In these results, the EtOAc fraction of bilberry was generally most excellent in all of the other fractions and the nitrite scavenging ability was found to be evidently most active in all of the other antioxidant systems.
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