In this study, in order to improve the anti-hyperglycemic activities of barley and wheat selected from 13 kinds of cereals, the two grains were soaked and germinated with tea catechin (TC) and chitooligosaccharides (COS). The anti-hyperglycemic and antioxidant activities of germinated barley (GBE) a...
In this study, in order to improve the anti-hyperglycemic activities of barley and wheat selected from 13 kinds of cereals, the two grains were soaked and germinated with tea catechin (TC) and chitooligosaccharides (COS). The anti-hyperglycemic and antioxidant activities of germinated barley (GBE) and wheat (GWE) extracts were measured on α-glucosidase, maltase, sucrase inhibitory activites, ORAC, reducing capacity and total phenolics content. According to the results, GBE and GWE showed a more potential activity when they were soaked and germinated with TC 0.5% and COS 1% at 25℃ for 24 hr. For the evaluation of the anti-diabetic and antioxidant activity of different kinds of barley and wheat at the optimum germinated condition, four kinds of barley such as naked (NB), new naked (NNB), Seodun waxy (SWB), and Hyemi unhulled barley (HUB) and three kinds of wheat such as whole (WHW), Gumgang (GUW), winter wheat (WIW) were selected and germinated. The radical scavenging activity of barley and wheat on peroxyl radical generated from AAPH were found at 2 mg/mL of fresh weight. The peroxyl radical-scavenging activity of NB was 48.55 TE (Trolox Equivalents, µM) which was the strongest among samples tested. The rat α-glucosidase, maltase, and sucrase inhibitory activities of barley and wheat extract at 5 mg/mL of fresh weight were evaluated. NB also showed the highest inhibitory activity on rat α-glucosidase, maltase, and sucrase. However, there was no significant difference among the selected wheat. These results indicate that NB can be used for the development of functional food to control oxidative stress-related diseases and lower the blood glucose levels. Moreover, TC and COS could be used as an elicitor for the improvement of anti-diabetic and antioxidant activity during the germination of barley and wheat. GWE and GBE were investigated as a potent cereal-derived α–glucosidase inhibitor in diabetic db/db mice. The in vitro inhibitory activity of GWE and GBE on intestinal α–glucosidase, sucrase, and maltase was relatively weak compared to Acarbose used as a positive control. However, sugar loading test with starch, sucrose, and maltose showed that significantly reduced postprandial increases in blood glucose, indicating that they may act as an intestinal α–glucosidase, sucrase, and maltase inhibitor. These results suggests that GWE and GBE could be used in the development of nutraceutical foods to control the blood glucose levels of diabetic patients by inhibiting intestinal α–glucosidase with reduced side effects. In a feeding test of three weeks, naked barley (NB) and whole wheat (WHW) were selected and germinated with 0.5% of TC and 1% of COS at 25 ℃ for 24 h. 10% of GBE and 8% of GWE were mixed with other components (supplied by Erom, a company that produces the functional Saengshik in Korea) to make a functional Raw Food. In order to measure the blood glucose lowering effect and side effect reducing potential of the mixed Raw Food in vivo rat and db/db mice model. Rats and db/db mouse were divided into 4 groups (n=10), respectively and fed with different high starch diet by the making methods of AIN-93G rodent for 3 weeks. Group 1 was made as a negative control group and group 2 as a positive group that contained Acarbose at the concentration of 0.4g/kg. Group 3 and group 4 were test groups that contained the mixed sample (Raw Food) at the concentrations of 150g/kg (Sample-Low) and 300g/kg (Sample-High), respectively. The body weight, food intake, blood glucose level and Glycosylated hemoglobin (HbA1c) level were measured. In addition, blood insulin, triglyceride (TG), total cholesterol (TC), High-density lipoprotein cholesterol (HDL-C), GPT, GOT and side-effects reducing potential in rats and db/db mouse were determined. Sample-low and sample-high groups showed 5% and 17% decrease of blood glucose level compared to the control group in db/db mouse. However, there were no significant changes in a rat model. Moreover, Sample-high group had a 13% decrease of the glycosylated hemoglobin (HbA1c) level compared to control in db/db mouse model. The treatment also resulted in a significant reduction of TG, TC, GOT and GPT activities of sample-low and sample-high groups when compared to control group. These results indicated that the Raw Food which contains GBE and GWE can not only be used to control the blood glucose level for diabetes but also be very beneficial to human’s health. In the 3-week feeding tests, the Acarbose-supplemented high-starch diet group (400 mg/kg diet weight) showed several symptoms of side-effects such as enlarged caecal volume and weight and reduced intestinal transport capacity. However, the sample-high group had fewer side-effects compared with the Acarbose group. These results suggest that the mixed Raw Food with lowering blood glucose effects that elicits less pronounced side effects has the potential to contribute to a useful dietary strategy for diabetes. Moreover, these results provide the basis for further human clinical studies.
In this study, in order to improve the anti-hyperglycemic activities of barley and wheat selected from 13 kinds of cereals, the two grains were soaked and germinated with tea catechin (TC) and chitooligosaccharides (COS). The anti-hyperglycemic and antioxidant activities of germinated barley (GBE) and wheat (GWE) extracts were measured on α-glucosidase, maltase, sucrase inhibitory activites, ORAC, reducing capacity and total phenolics content. According to the results, GBE and GWE showed a more potential activity when they were soaked and germinated with TC 0.5% and COS 1% at 25℃ for 24 hr. For the evaluation of the anti-diabetic and antioxidant activity of different kinds of barley and wheat at the optimum germinated condition, four kinds of barley such as naked (NB), new naked (NNB), Seodun waxy (SWB), and Hyemi unhulled barley (HUB) and three kinds of wheat such as whole (WHW), Gumgang (GUW), winter wheat (WIW) were selected and germinated. The radical scavenging activity of barley and wheat on peroxyl radical generated from AAPH were found at 2 mg/mL of fresh weight. The peroxyl radical-scavenging activity of NB was 48.55 TE (Trolox Equivalents, µM) which was the strongest among samples tested. The rat α-glucosidase, maltase, and sucrase inhibitory activities of barley and wheat extract at 5 mg/mL of fresh weight were evaluated. NB also showed the highest inhibitory activity on rat α-glucosidase, maltase, and sucrase. However, there was no significant difference among the selected wheat. These results indicate that NB can be used for the development of functional food to control oxidative stress-related diseases and lower the blood glucose levels. Moreover, TC and COS could be used as an elicitor for the improvement of anti-diabetic and antioxidant activity during the germination of barley and wheat. GWE and GBE were investigated as a potent cereal-derived α–glucosidase inhibitor in diabetic db/db mice. The in vitro inhibitory activity of GWE and GBE on intestinal α–glucosidase, sucrase, and maltase was relatively weak compared to Acarbose used as a positive control. However, sugar loading test with starch, sucrose, and maltose showed that significantly reduced postprandial increases in blood glucose, indicating that they may act as an intestinal α–glucosidase, sucrase, and maltase inhibitor. These results suggests that GWE and GBE could be used in the development of nutraceutical foods to control the blood glucose levels of diabetic patients by inhibiting intestinal α–glucosidase with reduced side effects. In a feeding test of three weeks, naked barley (NB) and whole wheat (WHW) were selected and germinated with 0.5% of TC and 1% of COS at 25 ℃ for 24 h. 10% of GBE and 8% of GWE were mixed with other components (supplied by Erom, a company that produces the functional Saengshik in Korea) to make a functional Raw Food. In order to measure the blood glucose lowering effect and side effect reducing potential of the mixed Raw Food in vivo rat and db/db mice model. Rats and db/db mouse were divided into 4 groups (n=10), respectively and fed with different high starch diet by the making methods of AIN-93G rodent for 3 weeks. Group 1 was made as a negative control group and group 2 as a positive group that contained Acarbose at the concentration of 0.4g/kg. Group 3 and group 4 were test groups that contained the mixed sample (Raw Food) at the concentrations of 150g/kg (Sample-Low) and 300g/kg (Sample-High), respectively. The body weight, food intake, blood glucose level and Glycosylated hemoglobin (HbA1c) level were measured. In addition, blood insulin, triglyceride (TG), total cholesterol (TC), High-density lipoprotein cholesterol (HDL-C), GPT, GOT and side-effects reducing potential in rats and db/db mouse were determined. Sample-low and sample-high groups showed 5% and 17% decrease of blood glucose level compared to the control group in db/db mouse. However, there were no significant changes in a rat model. Moreover, Sample-high group had a 13% decrease of the glycosylated hemoglobin (HbA1c) level compared to control in db/db mouse model. The treatment also resulted in a significant reduction of TG, TC, GOT and GPT activities of sample-low and sample-high groups when compared to control group. These results indicated that the Raw Food which contains GBE and GWE can not only be used to control the blood glucose level for diabetes but also be very beneficial to human’s health. In the 3-week feeding tests, the Acarbose-supplemented high-starch diet group (400 mg/kg diet weight) showed several symptoms of side-effects such as enlarged caecal volume and weight and reduced intestinal transport capacity. However, the sample-high group had fewer side-effects compared with the Acarbose group. These results suggest that the mixed Raw Food with lowering blood glucose effects that elicits less pronounced side effects has the potential to contribute to a useful dietary strategy for diabetes. Moreover, these results provide the basis for further human clinical studies.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.