加味大羌活湯은 風寒濕으로 인한 歷節風에 사용하는 大羌活湯에 鎭痛, 解毒의 효능이 있는 白屈菜, 淸熱解毒, 凉散風熱하는 효능을 지닌 金銀花, 淸熱解毒, 消腫散結의 효능을 지닌 蒲公英의 3가지 약재를 가미한 처방으로 임상적으로 활용되는 경험방이다. 이번 실험에서는 加味大羌活湯이 자이모산으로 유도된 급성 관절염 마우스 및 랫드 모델에 미치는 소염효과를 측정하고, 콜라겐 주입으로 유도된 만성 ...
加味大羌活湯은 風寒濕으로 인한 歷節風에 사용하는 大羌活湯에 鎭痛, 解毒의 효능이 있는 白屈菜, 淸熱解毒, 凉散風熱하는 효능을 지닌 金銀花, 淸熱解毒, 消腫散結의 효능을 지닌 蒲公英의 3가지 약재를 가미한 처방으로 임상적으로 활용되는 경험방이다. 이번 실험에서는 加味大羌活湯이 자이모산으로 유도된 급성 관절염 마우스 및 랫드 모델에 미치는 소염효과를 측정하고, 콜라겐 주입으로 유도된 만성 류마티스 관절염 마우스 모델에서 관절염 지수, 관절의 손상 및 염증상태에 미치는 효과를 보기 위해 혈청 내에서 면역글로블린, 자가항체 농도, 염증성 사이토카인 농도 등을 측정하고, 조직염색 및 방사선조사법(micro-CT법) 등을 이용하여 조직학적 변화 등을 관찰하였다. 그 결과 加味大羌活湯은 급성 염증성 관절염모델에서 고농도의 GDGHT를 투여한 경우 유의하게 백혈구 총수 및 TNF-α 분비를 억제하였고 족부 부종 억제능을 나타내었으며, type Ⅱ collagen 유발 만성 류마티스 관절염 마우스에서 관절염의 염증 소견을 유의하게 개선시키는 것으로 나타났으며, 특히 항염증 효능이 효과적인 것을 확인하였다. 따라서 향후 지속적인 추가연구를 통하여 加味大羌活湯을 임상에서 류마티스 관절염에 치료에 사용될 수 있을 것으로 사료된다.
加味大羌活湯은 風寒濕으로 인한 歷節風에 사용하는 大羌活湯에 鎭痛, 解毒의 효능이 있는 白屈菜, 淸熱解毒, 凉散風熱하는 효능을 지닌 金銀花, 淸熱解毒, 消腫散結의 효능을 지닌 蒲公英의 3가지 약재를 가미한 처방으로 임상적으로 활용되는 경험방이다. 이번 실험에서는 加味大羌活湯이 자이모산으로 유도된 급성 관절염 마우스 및 랫드 모델에 미치는 소염효과를 측정하고, 콜라겐 주입으로 유도된 만성 류마티스 관절염 마우스 모델에서 관절염 지수, 관절의 손상 및 염증상태에 미치는 효과를 보기 위해 혈청 내에서 면역글로블린, 자가항체 농도, 염증성 사이토카인 농도 등을 측정하고, 조직염색 및 방사선조사법(micro-CT법) 등을 이용하여 조직학적 변화 등을 관찰하였다. 그 결과 加味大羌活湯은 급성 염증성 관절염모델에서 고농도의 GDGHT를 투여한 경우 유의하게 백혈구 총수 및 TNF-α 분비를 억제하였고 족부 부종 억제능을 나타내었으며, type Ⅱ collagen 유발 만성 류마티스 관절염 마우스에서 관절염의 염증 소견을 유의하게 개선시키는 것으로 나타났으며, 특히 항염증 효능이 효과적인 것을 확인하였다. 따라서 향후 지속적인 추가연구를 통하여 加味大羌活湯을 임상에서 류마티스 관절염에 치료에 사용될 수 있을 것으로 사료된다.
Objectives: This study was carried out to investigate the anti-inflammatory and curative effects of Gami-daeganghwal-tang (Jiaweidaqianghuo-tang, hereinafter called to GDGHT) on the acute and chronic rheumatoid arthritis model. Methods: The acute anti-inflammatory effect of GDGHT was evaluated in zy...
Objectives: This study was carried out to investigate the anti-inflammatory and curative effects of Gami-daeganghwal-tang (Jiaweidaqianghuo-tang, hereinafter called to GDGHT) on the acute and chronic rheumatoid arthritis model. Methods: The acute anti-inflammatory effect of GDGHT was evaluated in zymosan induced paw edema model and leukocyte migration into air pouch model. GDGHT(50, 100, 200 mg/kg) was orally pretreated at 1 hour before zymosan injection. On the other hand, the rheumatoid arthritis was established by collagen II injection into DBA/1J male mice. Mice were divided into five groups as non-treated normal group, collagen II-immunized arthritic control group(CIA), and groups medicated with GDGHT 100 and 200 mg/kg and MTX for 21 days after 2nd collagen II immunization. The clinical signs such as body weight, paw edema thickness, and arthritic index score were measured every weeks to observe the incidence of arthritis after immunization. Finally, the histopathological examination was performed on the ankle joint, and the serum levels of immunoglobulins, TNF-α, IL-1β and anti-collagen II IgG was analysed. Results: In acute inflammation model, the highest dose of GDGHT(200 mg/kg) significantly reduced zymosan induced paw edema and leukocyte migration accompanying with TNF-α elevation. In the chronic rheumatoid arthritis model, the arthritis index score and paw edema thickness in CIA mice was attenuated significantly by GDGHT administration in a dose-dependent manner. The serum levels of IgG2a, IgG2b, and IgM in GDGHT-treated groups also were significantly decreased as compared with CIA control. Moreover, GDGHT decreased effectively the levels of anti-collagen Ⅱ and proinflammatory cytokines TNF-α and IL-1β, in serum of mice. Histochemical staining and micro-CT observation revealed that the degree of arthritis showing the joint damage in CIA mice was inhibited in GDGHT-treated groups. Conclusion: These results in this study showed that GDGHT had anti-inflammatory effects on the collagen-induced arthritis in mice through the inhibition of anti-collagen IgG and proinflammatory cytokines. Thus it is suggested that GDGHT should be used as an effective drug for rheumatoid arthritis and another inflammatory disease. Therefore it is needed to be surveyed continuously in looking for the anti-arthritic mechanism of GDGHT in the future.
Objectives: This study was carried out to investigate the anti-inflammatory and curative effects of Gami-daeganghwal-tang (Jiaweidaqianghuo-tang, hereinafter called to GDGHT) on the acute and chronic rheumatoid arthritis model. Methods: The acute anti-inflammatory effect of GDGHT was evaluated in zymosan induced paw edema model and leukocyte migration into air pouch model. GDGHT(50, 100, 200 mg/kg) was orally pretreated at 1 hour before zymosan injection. On the other hand, the rheumatoid arthritis was established by collagen II injection into DBA/1J male mice. Mice were divided into five groups as non-treated normal group, collagen II-immunized arthritic control group(CIA), and groups medicated with GDGHT 100 and 200 mg/kg and MTX for 21 days after 2nd collagen II immunization. The clinical signs such as body weight, paw edema thickness, and arthritic index score were measured every weeks to observe the incidence of arthritis after immunization. Finally, the histopathological examination was performed on the ankle joint, and the serum levels of immunoglobulins, TNF-α, IL-1β and anti-collagen II IgG was analysed. Results: In acute inflammation model, the highest dose of GDGHT(200 mg/kg) significantly reduced zymosan induced paw edema and leukocyte migration accompanying with TNF-α elevation. In the chronic rheumatoid arthritis model, the arthritis index score and paw edema thickness in CIA mice was attenuated significantly by GDGHT administration in a dose-dependent manner. The serum levels of IgG2a, IgG2b, and IgM in GDGHT-treated groups also were significantly decreased as compared with CIA control. Moreover, GDGHT decreased effectively the levels of anti-collagen Ⅱ and proinflammatory cytokines TNF-α and IL-1β, in serum of mice. Histochemical staining and micro-CT observation revealed that the degree of arthritis showing the joint damage in CIA mice was inhibited in GDGHT-treated groups. Conclusion: These results in this study showed that GDGHT had anti-inflammatory effects on the collagen-induced arthritis in mice through the inhibition of anti-collagen IgG and proinflammatory cytokines. Thus it is suggested that GDGHT should be used as an effective drug for rheumatoid arthritis and another inflammatory disease. Therefore it is needed to be surveyed continuously in looking for the anti-arthritic mechanism of GDGHT in the future.
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