Microsatellite is recognized as a useful genetic marker for its polymorphism, ease of genotyping, high reproducibility and ubiquity. It is generally accepted that microsatellite markers can be utilized in various kinds of genetic analyses with their locations on all kinds of cellular genomes, which ...
Microsatellite is recognized as a useful genetic marker for its polymorphism, ease of genotyping, high reproducibility and ubiquity. It is generally accepted that microsatellite markers can be utilized in various kinds of genetic analyses with their locations on all kinds of cellular genomes, which are nuclear, mitochondrial, and chloroplast genomes. The genus Eranthis (Ranunculaceae), which comprises about 8 perennial species, has not yet been studied with this useful molecular tool, microsatellite. The aim of this study is to develop chloroplast microsatellite markers for the genus Eranthis using two Eranthis species, E. byunsanensis and E. pungdoensis, and to analyze the polymorphism of these markers. Among the 40 primers initially designed using web based software, 24 primers were selected as useful markers with their high success rates of amplification in 8 individuals of E. byunsanensis, E. pungdoensis and with polymorphism. Mononucleotide repeat, with a repeat unit containing only one base pair was the most frequently found type in all of the developed markers. The polymorphism of the developed markers assessed in 40 individuals was evaluated with the measures of allele numbers and haplotypes. Among the 24 markers, the loci with 6 alleles were most polymorhpic and the loci with 2 alleles were least polymorphic. The averaged number of alleles per locus scored 3.42. In 30 individuals of E. byunsanensis, the averaged number of alleles per locus was 3.29, and in 10 individuals of E. pungdoensis, 1.13. When analyzed with 40 individuals, the number of haplotypes was 18. Above mentioned polymorphisms indicate that these newly developed markers can be useful in population genetics, the research of genetic diversity, population structure, and phylogeography. Moreover, the developed markers can be useful not only for these two species, E. byunsanensis, E. pungdoensis, but also for other species that belong to the genus Eranthis. It seems clear that the results of this study can lead to more efficient usage and conservation of the species in the genus Eranthis.
Microsatellite is recognized as a useful genetic marker for its polymorphism, ease of genotyping, high reproducibility and ubiquity. It is generally accepted that microsatellite markers can be utilized in various kinds of genetic analyses with their locations on all kinds of cellular genomes, which are nuclear, mitochondrial, and chloroplast genomes. The genus Eranthis (Ranunculaceae), which comprises about 8 perennial species, has not yet been studied with this useful molecular tool, microsatellite. The aim of this study is to develop chloroplast microsatellite markers for the genus Eranthis using two Eranthis species, E. byunsanensis and E. pungdoensis, and to analyze the polymorphism of these markers. Among the 40 primers initially designed using web based software, 24 primers were selected as useful markers with their high success rates of amplification in 8 individuals of E. byunsanensis, E. pungdoensis and with polymorphism. Mononucleotide repeat, with a repeat unit containing only one base pair was the most frequently found type in all of the developed markers. The polymorphism of the developed markers assessed in 40 individuals was evaluated with the measures of allele numbers and haplotypes. Among the 24 markers, the loci with 6 alleles were most polymorhpic and the loci with 2 alleles were least polymorphic. The averaged number of alleles per locus scored 3.42. In 30 individuals of E. byunsanensis, the averaged number of alleles per locus was 3.29, and in 10 individuals of E. pungdoensis, 1.13. When analyzed with 40 individuals, the number of haplotypes was 18. Above mentioned polymorphisms indicate that these newly developed markers can be useful in population genetics, the research of genetic diversity, population structure, and phylogeography. Moreover, the developed markers can be useful not only for these two species, E. byunsanensis, E. pungdoensis, but also for other species that belong to the genus Eranthis. It seems clear that the results of this study can lead to more efficient usage and conservation of the species in the genus Eranthis.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.