The purpose of this study was to evaluate and analyze the content and activities of antioxidant substances for the characterization of antioxidant physiologically active substances of extracts from Pinus densiflora Siebold & Zucc. The bark extracts of P. densiflora showed the highest antioxidative a...
The purpose of this study was to evaluate and analyze the content and activities of antioxidant substances for the characterization of antioxidant physiologically active substances of extracts from Pinus densiflora Siebold & Zucc. The bark extracts of P. densiflora showed the highest antioxidative activities in the bark ethanol extracts from Pinus species (P. densiflora, P. rigida, P. koraiensis, and P. thnbergii) and different by-products (bark, xylem, cones, and needles) from P. densiflora siebold & Zucc. The bark ethanol extract of P. densiflora was fractioned by solvents (hexane, chloroform, ethyl acetate, butyl alcohol, and distilled water) and the butyl alcohol fraction was the highest antioxidative activity. In the bark extracts of P. densiflora, total polyphenol content (TPC) of ethanol extract was 352 mg/g-extract and total flavonoid content (TFC) of ethyl acetate fraction was 39 mg/g-extract. Radical scavenging activity (half maximal effective concentration; EC50) was evaluated using DPPH (1,1-diphenyl-2- picrylhydrazyl) assay. EC50 of bark ethanol extract and ethyl acetate fraction from P. densiflora were 20, 31 ㎍-extract/mL, respectively and showed similar value with the ascorbic acid (20 ㎍·mL-1) and butylated hydroxyanisole (BHA=25 ㎍·mL-1), and were better than butylated hydroxytoluene (BHT=60 ㎍·mL-1). ECi sensitivity to DPPH radicals (DPPH•) of pine extracts may be affected more by TPC than TFC, the bark ethyl acetate and butyl alcohol fractions of P. densiflora showed low level ECi sensitivity to DPPH• and high radical scavenging activities on low concentration of extracts. The second-order rate constant (k2) on initial reaction to DPPH were evaluated that ascorbic acid (purified synthetic antioxidant control) was the highest value with 277.58 L/g-antioxidant min-1, but bark ethanol extract and butyl alcohol fraction in pine extracts were the highest k2 values with 29.64, 9.53 L/g-antioxidant min-1, respectively and showed higher k2 values than BHA and BHT (1.33, 0.45 L/g-antioxidant min-1). These results suggest that bark of P. densiflora Siebold & Zucc. may be useful phytoresources for the natural antioxidant physiologically active substance.
The purpose of this study was to evaluate and analyze the content and activities of antioxidant substances for the characterization of antioxidant physiologically active substances of extracts from Pinus densiflora Siebold & Zucc. The bark extracts of P. densiflora showed the highest antioxidative activities in the bark ethanol extracts from Pinus species (P. densiflora, P. rigida, P. koraiensis, and P. thnbergii) and different by-products (bark, xylem, cones, and needles) from P. densiflora siebold & Zucc. The bark ethanol extract of P. densiflora was fractioned by solvents (hexane, chloroform, ethyl acetate, butyl alcohol, and distilled water) and the butyl alcohol fraction was the highest antioxidative activity. In the bark extracts of P. densiflora, total polyphenol content (TPC) of ethanol extract was 352 mg/g-extract and total flavonoid content (TFC) of ethyl acetate fraction was 39 mg/g-extract. Radical scavenging activity (half maximal effective concentration; EC50) was evaluated using DPPH (1,1-diphenyl-2- picrylhydrazyl) assay. EC50 of bark ethanol extract and ethyl acetate fraction from P. densiflora were 20, 31 ㎍-extract/mL, respectively and showed similar value with the ascorbic acid (20 ㎍·mL-1) and butylated hydroxyanisole (BHA=25 ㎍·mL-1), and were better than butylated hydroxytoluene (BHT=60 ㎍·mL-1). ECi sensitivity to DPPH radicals (DPPH•) of pine extracts may be affected more by TPC than TFC, the bark ethyl acetate and butyl alcohol fractions of P. densiflora showed low level ECi sensitivity to DPPH• and high radical scavenging activities on low concentration of extracts. The second-order rate constant (k2) on initial reaction to DPPH were evaluated that ascorbic acid (purified synthetic antioxidant control) was the highest value with 277.58 L/g-antioxidant min-1, but bark ethanol extract and butyl alcohol fraction in pine extracts were the highest k2 values with 29.64, 9.53 L/g-antioxidant min-1, respectively and showed higher k2 values than BHA and BHT (1.33, 0.45 L/g-antioxidant min-1). These results suggest that bark of P. densiflora Siebold & Zucc. may be useful phytoresources for the natural antioxidant physiologically active substance.
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