본 연구에서는 3 종의 성장인자(EGF, bFGF, VEGF)를 이용하여 사람 섬유아세포에서의 세포 증식률과 콜라겐 합성 효과를 평가하고자 하였다. 사람섬유아세포인 CCD-18Co 세포에서 세포 증식률을 확인하기 위하여 ...
본 연구에서는 3 종의 성장인자(EGF, bFGF, VEGF)를 이용하여 사람 섬유아세포에서의 세포 증식률과 콜라겐 합성 효과를 평가하고자 하였다. 사람섬유아세포인 CCD-18Co 세포에서 세포 증식률을 확인하기 위하여 MTT assay 방법을 이용하였고, 단독 적용했을 때보다 3 종 성장인자(EGF 20 ng/ml, bFGF 20 ng/ml, VEGF 20 ng/ml)를 함께 복합 적용하였을 때 세포 증식률이 유의적으로 증가하는 것을 확인할 수 있었다. 또한 콜라겐 합성 효능을 알아보기 위하여 Sircol collagen assay kit를 사용하여 가용성 콜라겐을 측정한 결과 세포 증식률과 마찬가지로 단독 적용했을 때 보다 3 종 성장인자(EGF 20 ng/ml, bFGF 20 ng/ml, VEGF 20 ng/ml)를 복합 적용하였을 때 콜라겐 생성량이 대조군과 비교하여 약 6배 증가한다는 것을 확인할 수 있었다. 그러므로 EGF, bFGF 및 VEGF는 섬유아세포의 증식과 콜라겐 합성을 촉진하는 효능이 있음을 확인하였으며, 의약품, 기능성화장품 및 생리활성물질로 활용이 가능할 것으로 생각한다.
본 연구에서는 3 종의 성장인자(EGF, bFGF, VEGF)를 이용하여 사람 섬유아세포에서의 세포 증식률과 콜라겐 합성 효과를 평가하고자 하였다. 사람섬유아세포인 CCD-18Co 세포에서 세포 증식률을 확인하기 위하여 MTT assay 방법을 이용하였고, 단독 적용했을 때보다 3 종 성장인자(EGF 20 ng/ml, bFGF 20 ng/ml, VEGF 20 ng/ml)를 함께 복합 적용하였을 때 세포 증식률이 유의적으로 증가하는 것을 확인할 수 있었다. 또한 콜라겐 합성 효능을 알아보기 위하여 Sircol collagen assay kit를 사용하여 가용성 콜라겐을 측정한 결과 세포 증식률과 마찬가지로 단독 적용했을 때 보다 3 종 성장인자(EGF 20 ng/ml, bFGF 20 ng/ml, VEGF 20 ng/ml)를 복합 적용하였을 때 콜라겐 생성량이 대조군과 비교하여 약 6배 증가한다는 것을 확인할 수 있었다. 그러므로 EGF, bFGF 및 VEGF는 섬유아세포의 증식과 콜라겐 합성을 촉진하는 효능이 있음을 확인하였으며, 의약품, 기능성화장품 및 생리활성물질로 활용이 가능할 것으로 생각한다.
Growth factors have been found in almost all of the tissues and are known to stimulate cellular growth, proliferation, migration and differentiation. In this study, their functions on cellular proliferation and collagen synthesis in human fibroblasts were investigated especially using epithelial...
Growth factors have been found in almost all of the tissues and are known to stimulate cellular growth, proliferation, migration and differentiation. In this study, their functions on cellular proliferation and collagen synthesis in human fibroblasts were investigated especially using epithelial growth factor (EGF), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), which are typical growth factors. Human fibroblast cells were treated with growth factors (EGF, bFGF, VEGF) alone or together at concentration of 2~100 ng/ml for 48 hours. When the fibroblasts were treated with 20 ng/ml of EGF, 20 ng/ml of bFGF or 20 ng/ml of VEGF, respectively, they showed high proliferation rates. Further, the fibroblasts showed the highest proliferation rate when the triple growth factors were treated simultaneously. Collagen production of the fibroblasts was considerably increased when two growth factors were treated compared with that of single treatment. Triple treatment of growth factors showed the highest rate on the collagen synthesis on human fibroblasts. Based on the results concerning increased proliferation and collagen synthesis of human fibroblasts by combined treatment of EGF, bFGF and VEGF, these growth factors can be applied as valuable ingredients for cellular proliferation and cosmetic field including bioactive compounds.
Growth factors have been found in almost all of the tissues and are known to stimulate cellular growth, proliferation, migration and differentiation. In this study, their functions on cellular proliferation and collagen synthesis in human fibroblasts were investigated especially using epithelial growth factor (EGF), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), which are typical growth factors. Human fibroblast cells were treated with growth factors (EGF, bFGF, VEGF) alone or together at concentration of 2~100 ng/ml for 48 hours. When the fibroblasts were treated with 20 ng/ml of EGF, 20 ng/ml of bFGF or 20 ng/ml of VEGF, respectively, they showed high proliferation rates. Further, the fibroblasts showed the highest proliferation rate when the triple growth factors were treated simultaneously. Collagen production of the fibroblasts was considerably increased when two growth factors were treated compared with that of single treatment. Triple treatment of growth factors showed the highest rate on the collagen synthesis on human fibroblasts. Based on the results concerning increased proliferation and collagen synthesis of human fibroblasts by combined treatment of EGF, bFGF and VEGF, these growth factors can be applied as valuable ingredients for cellular proliferation and cosmetic field including bioactive compounds.
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