Doxorubicin, one of the clinically most useful anticancer agents, is used alone or in combination with other drugs against a wide variety of tumors, recently. But cancer cells developed resistance to this agent in many ways. This resistance is an important limiting factor of doxorubicin for anticancer drug. We newly established doxorubicin-resistant HCT15/CL02 subline from parental HCT15 human adenocarcinoma colon cancer cells. HCT15/CL02 revealed resistance to doxorubicin about 85-fold of its parental cells, and it also revealed cross-resistance to actinomycin D, etoposide and vinblastine but not to displatin and tamoxifen. And verapamil, a reversal agent of multidrug-resistance (MDR) by P-glycoprotein, elevated the cytotoxicity of doxorubicin against both HCT15 and GCT15/CL02 cells. But the relative resistant rate was not reduced. Verapamil had no effects on the tosicity of cisplatin to the both cell lines. These results indicate that HCT15/CL02 cells have some functionally complex mechanisms for MDR.
2002. "Isolation and Properties of Cytotoxic Polyene Antibiotics Produced by Myxococcus stipitatus JW117." 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 30(2): 157~161
2002. "Isolation and Properties of Cytotoxic Antibiotics Produced by Myxococcus stipitatus JW150" 약학회지 = Yakhak hoeji, 46(2): 108~112
2004. "Isolation of Antibiotics Effective to Multidrug-Resistant Cancer Cells from Sorangium cellulosum(Myxobacteria)." 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 32(1): 47~51
Ahn Jong-Woong ; Choi Sang-Un ; Seo Youngwan ; Rho Jung-Rae 2005. "Chivosazole F, An Efficient Inhibitor of Multidrug-Resistant Cancer Cells Isolated from Sorangium Cellulosum [Myxobacteria]" 한국생물공학회지 = Korean journal of biotechnology and bioengineering, 20(5): 371~375
Cho, Jung-Ah ; Lee, Jae-Tae ; Yoo, Jung-Ah ; Seo, Ji-Hyoung ; Bae, Jin-Ho ; Jeong, Shin-Young ; Ahn, Byeong-Cheol ; Sohn, Sang-Gyun ; Ha, Jeoung-Hee ; Lee, Kyu-Bo 2005. "Comparative Uptake of Tc-99m Sestamibi and Tc-99m Tetrofosmin in Cancer Cells and Tissue Expressing P-Glycoprotein or Multidrug Resistance Associated Protein" 大韓核醫學會誌 = Korean journal of nuclear medicine, 39(1): 34~43
2009. "" Journal of the Korean Society for Applied Biological Chemistry, 52(4): 375~379