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논문 상세정보

Abstract

As basic study to evaluate the treatability of oil-contaminated environment with bacteria, isolation and characterization of crude oil-degrading bacterium were carried out. A bacterial strain SH-14 capable of degrading crude oil was isolated from contaminated soils by enrichment culture technique and identified as Acinetobacter sp. by morphological, cultural and biochemical characteristics, and so named Acinetobacter sp. SH-14. The optimal medium composition and cultural conditions for the growth and emulsification of crude oil by Acinetobacter sp. SH-14 used were crude oil of 2.0%, $KNO_3$ of 0.2%, $K_2HPO_4$ of 0.05%, and $MgSO_4\;{\cdot}\;7H_2O$ of 1.0%, along with initial pH 7.0 at $30^{\circ}C$. Acinetobacter sp. SH-14 showed to be resistant to chloramphenicol and utilized various hydrocarbons such as dodecane, hexadecane, isooctane, cyclo-hexane etc., as a sole carbon source. Acinetobacter sp. SH-14 harbored a single plasmid. By agarose gel electrophoresis and curing experiment it was found that the genes for crude oil components degradation were encoded on the plasmid.

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이 논문을 인용한 문헌 (4)

  1. Lee, Chang-Ho ; Kim, Hee-Sik ; Suh, Hyun-Hyo ; Choi, Soung-Hun ; Oh, Hee-Mock ; Yoon, Byung-Dae 1997. "" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology, 25(5): 520~526 
  2. 1999. "Characterization of Crude Oil Degradation by Klebsiella sp. KCL-1 Isolated from Sea Water" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology, 27(6): 452~457 
  3. 2000. "Characterization of Crude Oil Degradation by Klebsiella sp. KCL-2 Isolated from Sea Water" 생명과학회지 = Journal of life science, 10(3): 300~306 
  4. 2002. "Characteristics of Biosurfactants produced by Bacillus sp. LSC11" 생명과학회지 = Journal of life science, 12(6): 745~751 

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