Abstract

As basic study to evaluate the treatability of oil-contaminated environment with bacteria, isolation and characterization of crude oil-degrading bacterium were carried out. A bacterial strain SH-14 capable of degrading crude oil was isolated from contaminated soils by enrichment culture technique and identified as Acinetobacter sp. by morphological, cultural and biochemical characteristics, and so named Acinetobacter sp. SH-14. The optimal medium composition and cultural conditions for the growth and emulsification of crude oil by Acinetobacter sp. SH-14 used were crude oil of 2.0%, $KNO_3$ of 0.2%, $K_2HPO_4$ of 0.05%, and $MgSO_4\;{\cdot}\;7H_2O$ of 1.0%, along with initial pH 7.0 at $30^{\circ}C$. Acinetobacter sp. SH-14 showed to be resistant to chloramphenicol and utilized various hydrocarbons such as dodecane, hexadecane, isooctane, cyclo-hexane etc., as a sole carbon source. Acinetobacter sp. SH-14 harbored a single plasmid. By agarose gel electrophoresis and curing experiment it was found that the genes for crude oil components degradation were encoded on the plasmid.

주제어

#Acrinettobacter sp   #SH-14 crude oil   #hydrocarbon   #bioddegradationl emulsification  

저자의 다른 논문

참고문헌 (27)

1. Biodegradation of petroleum in seawater at low temperatures , Atlas, R.M.;R. Bartha , Can. J. Microbiol. / v.18,pp.1851-1855, 1972
   
2. Effects of oil on bacterial activity in marine and freshwater sediments , Baker, J.H.;R.P. Griffiths;M.J. Klug(ed.);C.A. Reddy(ed.) , Current Perspective in Microbial Ecology / v.,pp.546-551, 1984
   
3. Oil pollution of the marine environment , Carlberg, S.R.;E. Olausson(ed.);I. Cato(ed.) , Chemistry and biogeochemistry of estuaries / v.,pp.367-402, 1980
   
4. Genetic regulation of octane dissimilation plasmid in Pseudomonas , Chakarabarty, A.M.;G. Chon;I.C. Gunsalus , Proc. Nat. Acad. Sci. USA / v.70,pp.1137-1140, 1973
   
5. Petroleum: properties and analyses in biotic and abiotic systems , Clark, R.C.;D.W. Brown;D.C. Malins(ed.) , Effects of petroleum on arctic and subartic marine environments and organisms / v.,pp.1-89, 1977
   
6. Microbial protein from hydrocarbons , Coty, V.F.;R.I. Leavitt;E.D. Murray(ed.) , Developments in industrial microbiology / v.12,pp.61-70, 1971
   
7. Survival of lux-lac-marked biosurfactant-producing Pseudomonas aeruginosa UG2L in soil monitored by nonselective plating and PCR , Flemming, C.A.;K.T. Leung;H. Lee;J.T. Trevors;C.W. Greer , Appl. Environ. Microbiol. / v.60,pp.1606-1613, 1994
   
8. Degradation of polycyclic aromatic hydrocarbons and aromatic heterocycles by a Pseudomonas species , Foght, J.M.;D.W.S. Westlake , Can. J. Microbiol. / v.34,pp.1135-1141, 1988
   
9. Hydrocarbons as substrates for microorganisms , Foster, J.W.;Antonie van Lee-wenhoek , J. Microbiol. Serol. / v.28,pp.241-274, 1962
   
10. Cloning and heterologous expression of a gene encoding an alkane-induced extracellular protein involved in alkane assimilation from Pseudomonas aeruginosa , Hardegger, M.;A.K. Koch;U.A. Ochsner;A. Fiechter;J. Reiser , Appl. Environ. Microbiol. / v.60,pp.3679-3687, 1994
    
11. Sequential growth of bacteria on crude oil , Horowitz, A.;D. Gutnick;E. Rogenberg , Appl. Microbiol. / v.30,pp.10-19, 1975
    
12. Microbial utilization of crude oil , Jobson, A.;F.D. Cook;D.W.S. Westlake , Appl. Microbiol. / v.23,pp.1082-1089, 1972
    
13. Microbial degradation of alkane components in crude oil , Kim, S.H.;C.S. Kim;I.S. Cho;S.Y. Choi;K.H. Min , Kor. J. Microbiol. / v.28,pp.71-75, 1990
    
14. Krieg, N.R.;J.G. Holt , Bergey's Manual of Systematic Bacteriology / v.1,pp., 1984
    
15. The mechanisms of microbial oxidation of petroleum hydrocarbons , Linden Van Liden., A.C.;G.J.E. Thijsse , Advan. Enzymol. / v.27,pp.469-546, 1965
    
16. Sources of petroleum hydrocarbon inputs to the marine environment , Payne, F.G.;M.T. Westaway , Water Sci. Technol. / v.14,pp.1159-1170, 1982
    
17. Role for emulsan in growth of Acinetobacter calcoaceticus RAG-1 on crude oil , Pines, O.;D. Gutnick , Appl. Environ. Microbiol. / v.51,pp.661-663, 1986
    
18. Predominant role of hydrocarbon solublization in the microbial uptake of hydrocarbons , Reddy, P.G.;H.D. Singh;M.G. Bhagat;J.N. Baruah , Biotech. Bioeng. / v.24,pp.1241-1269, 1982
    
19. Microbial degradation of crude oil: Factors affecting the dispersion in seawater by mixed and pure culture , Resfeld, A.;E. Rosenberg;D. Gutnick , Appl. Microbiol. / v.24,pp.363-368, 1992
    
20. Involvement of a plasmid in growth on and dispersion of crude oil by Acinetobacter calcoaceticus RA57 , Rusansky, S.;R. Avigad;S. Michaell;D.L. Gutnick , Appl. Environ. Microbiol. / v.53,pp.1918-1923, 1987
    
21. Sambrook, J.;E.F. Fritsch;T. Maniatis , Molecular Cloning-a laboratory manual / v.,pp., 1989
    
22. Genetics of hydrocarbon-utilizing microorganisms , Singer, J.T.;W.R. Finnerty;R.M. Atlas(ed.) , Petroleum Microbiology / v.,pp.299-354, 1984
    
23. Production of bacterial cell from hydrocarbons , Takahashi, J.;K. Koboyashi;Y. Kawabata;K. Yamada , Agr. Biol. Chem. / v.12,pp.836-842, 1963
    
24. Practical considerations in the surfactant-aided mobilization of contaminants in acquifers , Vigon, B.W.;A.J. Rubin , J. Water Pollut. Control Fed. / v.61,pp.1233-1239, 1989
    
25. Microbial petroleum degradation: application of computerized mass spectrometry , Walker, J.D.;R.R. Colwell;L. Petrakis , Can. J. Microbiol. / v.21,pp.1760-1767, 1975
    
26. Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidense for the existence of new TOL plasmids , Williams, P.A.;M.J. Worsey , J. Bacteriol. / v.343,pp.552-560, 1976
    
27. Characterizarion of a spontaneously occurring mutant of the TOL 20 plasmid in Pseudomonas putida MT20: possible regulatory implications , Worsey, M.J.;P.A. Williams , J. Bacteriol. / v.131,pp.1149-1158, 1977
    

이 논문을 인용한 문헌 (4)

1. Lee, Chang-Ho ; Kim, Hee-Sik ; Suh, Hyun-Hyo ; Choi, Soung-Hun ; Oh, Hee-Mock ; Yoon, Byung-Dae 1997. "" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology, 25(5): 520~526  원문보기 상세보기
2. 1999. "Characterization of Crude Oil Degradation by Klebsiella sp. KCL-1 Isolated from Sea Water" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology, 27(6): 452~457  원문보기 상세보기
3. 2000. "Characterization of Crude Oil Degradation by Klebsiella sp. KCL-2 Isolated from Sea Water" 생명과학회지 = Journal of life science, 10(3): 300~306  원문보기 상세보기
4. 2002. "Characteristics of Biosurfactants produced by Bacillus sp. LSC11" 생명과학회지 = Journal of life science, 12(6): 745~751  원문보기 상세보기