초록

AI-Helper

zooglan생합성에 필수적인 gene cluster를 clone하기 위해 2종류의 변이주가 분리되었다. Zoogloea ramigera 115는 협막형 다당(주로 zooglan)을 생산한다. 115 균주로 접합시키고 생산물을 용이하게 분리하기 위하여 반복된 원심분리와 선별을 통해 협막을 만들지 않는 slime형 생산균주를 분리하였다. 세포외 다당 생산 능력이 결여된 변이주를 전통적인 transposon(Tn5) 기술을 사용하여 얻었고 달라진 colony 형태와 celluflour결합 성질에 의해 선별하였다. 이들 변이주들은 범용숙주범위 cosmid vector안에 건설된 Z.ramigera 115slime gene library 와 helper plasmid로의 3양친 접합에 의해 보상되었다.

Abstract ▼ AI-Helper

Two kinds of mutants were isolated to clone a cluster of genes essential for zooglan biosynthesis. Zoogloea ramigera 115 strains produce capsular polysaccharide. To achieve conjugation in strain 115 and to facilitate recovery of product, a capsule non-forming strain was isolated via successive cent...

Two kinds of mutants were isolated to clone a cluster of genes essential for zooglan biosynthesis. Zoogloea ramigera 115 strains produce capsular polysaccharide. To achieve conjugation in strain 115 and to facilitate recovery of product, a capsule non-forming strain was isolated via successive centrifugation and screening. The other kind of mutants devoid of or producing altered exopolysaccharides were obtained using classical transposon(Tn5) technique and screened for altered colony morphology and celluflour binding properties. Complementation of these mutants was achieved with Z. ramigera 115 slime gene library constructed in a broad host range cosmid vector and helper plasmid by triparental conjugation.