초록

여름느타리버섯의 담자포자에 자외선을 조사하여 5'-fluoro-orotic acid(FOA) 선발배지에서 pyrimidine 영양요구성 균주를 직접 선발하였다. 24개의 5'-FOA 저항성 균주중 13개의 pyrimidine 영양요구성 균주를 선발할 수 있었으며, 이들의 mating type group과 성장속도를 측정하여 형질전환을 위한 최적의 DNA수용체를 선발할 수 있었다.

Abstract

Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5'-fluoro-orotic acid (5'-FOA) whereas pyrimidine auxotrophs grew normally. After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5'-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.

주제어

#5'-Fluoro-orotic acid   #Pyrimidine auxotrophic mutant   #U   #V mutagenesis   #Pleurotus sajor-caju   #Basidiomycotina  

저자의 다른 논문

참고문헌 (11)

1. Transformation of Aspergillus nidulans by the orotidine 5'-monophosphate decarboxylase gene of Neurospora crassa , Ballance, D.J.;Buxton, F.P.;Tuner, G , Biochem. Biophysic. Res. Commun. / v.112,pp.284-289, 1983
   
2. A positive selection for mutants lacking orotidine-5'-phosphate decaboxyase activity in yeast: 5-fluoro-orotic acid resistance , Boeke, J.D.;Croute, F.;Finik, G.R. , Mol. Gen. Genet. / v.197,pp.345-346, 1984
   
3. Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus , Campuzano, V.;Valle, del P.;Vicente, de J.I.;Eslava, A.P.;Alvarez, M.I. , Curr. Genet. / v.24,pp.515-519, 1993
   
4. Selection of Pleurotus sajor-caju suitable species for cultivation under summer climatic conditions in Korea , Go, S.J.;Byun, M.O.;You, C.H.;Park, Y.H. , Korean J. Mycol. / v.12,pp.53-58, 1984
   
5. Transformation of Aspergillus niger using the homologous orotidine-5'-monophosphate-decarboxylase gene , Goosen, T.;Bloemheuvel, G.;Gysler, C.;Debie, D.A.;Broek, H.J. Vander;Klaas swart. , Curr. Genet. / v.11,pp.499-503, 1987
   
6. Isolation of auxotrophic mutants from basidiospores of Pleurotus cornucopiae , Lee, Y.H.;Park, Y.H.;Yoo, Y.B.;Min, K.H. , Korean J. Mycol. / v.14,pp.185-188, 1986
   
7. Homologous and heterologous gene transfer systems in basidiomycetes , Noel, T.;Labarere, J. , Mycoscience / v.36,pp.127-133, 1995
   
8. Recovery of recombinant plasmids from Pleurotus ostreatus transformants , Peng, M.;Singh, N.K.;Lemke, P.A. , Curr. Genet / v.22,pp.53-59, 1992
   
9. Genetic analysis of the life cycle of Agaricus bisporus , Raper, C.A,;Raper, J.R.;Miller, R.E. , Mycologia / v.64,pp.1088-1117, 1972
   
10. Sexuality of an edible mushroom Pleurotus sajor-caju , Roxon, J.E.;Jong, S.C. , Mycologia / v.69,pp.203-205, 1977
    
11. Isolation of auxotrophic mutants from basidiospores of Lentinus edodes , Yoo, Y.B.;You, C.H.;Park, Y.H. , Korean J. Mycol. / v.13,pp.185-189, 1985
    

이 논문을 인용한 문헌 (0)

1. 이 논문을 인용한 문헌 없음