$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

생물학적 특성과 DNA분석을 이용한 한국내 Armillaria속균의 분류

Taxonomic Study of Korean Armillaria Species Based on Biological Characteristics and DNA Analyses

초록

1985년부터 1993년까지 장원도 일대에서 채집한 20균주와 외부에서 분양 받은 6균주를 대상으로 교배실험, PCR-RFLP 분석, 그리고 RAPD분석을 통해 상호 유연 관계를 알고자 하였다. 교배실험에서는 A. gallica 6균주, A. ostoyae 5균주, A. tabescens 2균주만이 친화성을 나타내었다. A8(KNU-250)균주는 A. gallica와 A. ostoyae에 모두 친화성을 나타내어 배양적 특징과 유사한 결과가 나타났다. rDNA의 IGS영역의 PCR-RFLP분석에서 A. mellea와 A. ostoyae 각 균주들 간의 유사도는 1.000으로 100%의 상동성을 보여주었다. A. tabescens는 $0.919{\sim}0.974$로 높은 유사성을 나타냈다. A. gallica는 $0.619{\sim}1.000$의 유사도로 나타났다. A19와 A22균주는 고립된 한 집단으로 나타났으며 유사도는 1.000으로 100% 상동성을 보였고, 다른 집단과의 유사도가 $0.051{\sim}0.108$로 10% 미만의 상동성을 나타내고 있다. RAPD분석을 통한 A. mellea의 유사도는 $0.983{\sim}1.000$으로 상동성이 높게 나타났으며, A. ostoyae도 $0.994{\sim}1.000$으로 높은 상동성을 보여 주었다. A. tabescens의 균주간의 유사도는 $0.594{\sim}0.953$으로 나타났으며, A. gallica는 $0.280{\sim}0.733$의 유사도를 나타내 균주간의 변이가 나타난 것으로 생각된다. 특히, A19, A22균주가 다른 집단과 거리가 먼 한 집단으로 나타났는데 그들 간의 유사도는 0.921로 92%의 상동성을 보였고, 다른 4개 집단과의 유사도가 $0.012{\sim}0.069$로 7% 미만의 상동성을 나타내고 있다. 본 실험에서 분류된 Armillaria속균은 5종으로 4종은 각각 이미 알려진 종으로 A. mellea, A. ostoyae, A. tabescens, A. gallica로 동정되었고 남은 1종은 미기록종이라고 생각된다.

Abstract

From 1985 to 1993, we collected 20 isolates throughout Kangwon and obtained 6 isolates from other sources. A. mellea formed rhizomorph actively, and some of A. osroyae were poor in the formation of rhizomorph and some without formation of rhizomorph. A. tabescens was active in the growth of aerial mycelium and poor in the development of rhizomorph. In A. gallica, the mycelium development among the isolates were variable greatly, and especially in isolate A8(KNU-250), the mycelial development was similar to that of A. osroyae, but A8(KNU-250) showed the feature of A. gallica to change medium into brown color. In PCR-RFLP analysis of the IGS region in rDNA, the homology between each isolate in the A. mellea and A. ostoyae showed 100% homology. A. tabescens showed $0.919{\sim}0.974$ homology, and A. gallica showed $0.619{\sim}1.000$ homology. A19 and A12 showed 100% homology as the same group, but compared with other subgroups they showed less than 10% homology as $0.051{\sim}0.108$ value. In RAPD analyses, the isolates of A. mellea showed high homology among themselves as $0.983{\sim}1.000$, and A. ostoyae also showed high similarity. The homology between isolates of A. tabescens showed $0.594{\sim}0.953$ value because A. gallica showed $0.280{\sim}0.733$ value, and the variations between isolates were greater than other species. Especially, A19 and A22 were identified as new novel group which were remoted from other groups, and the homology between these two isolates showed 0.921 value, and the genetic similarity between these groups and other 4 groups showed less than 7% as $0.012{\sim}0.069$ value. Of 5 species identified in this study, 4 species were identified as A. mellea, A. ostoyae, A. tabescens, and A. gallica that were already reported ones and 1 species was suggested as a new specie in Korea.

참고문헌 (33)

  1. Biological species of Armillaria in North America: Redesignation of groups IV and VIII and enumeration of voucher strains for other groups , Anderson, J.B. , Mycologia / v.78,pp.837-839, 1986
  2. Restriction fragment length polymorphisms in biological species of Ann ill aria mellea , Anderson, J.B.;Petsche, D.M.;Smith, M.L. , Mycologia / v.79,pp.69-76, 1987
  3. Variation in ribosomal DNA among biological species of Annillaria, a genus of rootinfecting fungi , Anderson, J.B.;Bailey, S.S.;Pukkila, P.J. , Evolution / v.43,pp.1652-1662, 1989
  4. Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic DNA(RAPD) , Bidochka, M.J.;McDonald, M.A.;St. Leger, R.J.;Roberts, D.W. , Curr. Genet. / v.25,pp.107-113, 1994
  5. Serological differentiation of three species of Armillaria and Lentinula edodes by enzyme-linked immunosorbent assay using immunized chickens as a source of antibodies , Burdsall, H.H.;Banik, M. Jr.;Cook, M.E. , Mycologia / v.82,pp.415-423, 1990
  6. Rapid extraction of fungal DNA for PCR amplification , Cenis, J.L. , Nuc. Acids Res. / v.20,pp.2380, 1992
  7. Location and direction of transcription of the 5S rRNA gene in Armillaria , Duchesne, L.C.;Anderson, J.B. , Mycological Research / v.94,pp.266-269, 1990
  8. The polymerase chain reaction and its application to filamentous fungi , Foster, L.M.;Kozak, K.R.;Loftus, M.G.;Stevens, J.J.;Ross, I.K. , Mycol. Res. / v.97,pp.769-781, 1993
  9. Annillaria species in the northern temperate hemisphere , Guillaumin, J.J.;Mohammed, C.;Berthelay, S. , Proc. 7th Int. Conf. Root Butt Rots. / v.,pp.27-43, 1988
  10. Random amplified polymorphic DNA markers: A system for identifying and differentiating isolates of Colletotrichum graminicola , Guthrie, P.A.I.;Magill, C.W.;Frederikson,;Odvody, G.N. , Phytopathology / v.82,pp.832-835, 1992
  11. Molecular systematics , Hillis, D.M.;Moritz, C. , / v.,pp., 1990
  12. The use of L-DOPA to enhance visualization of the black line between species of the Armillaria mellea complex , Hopkin, A.A.;Mallett, K.I.;Blenis, P.V. , Can. J. Bot. / v.67,pp.15-17, 1989
  13. Restriction fragment length polymorphisms of mitochondrial DNA among Phytophthora capsici isolates from Pepper(Capsicum annuum) , Hwang, B.K.;De Cock, A.W.A.M.;Bahnweg, G.;Prell, H.H.;Heitefuss, R. , System. Appl. Microbiol. / v.14,pp.111-116, 1991
  14. Breeding units in the forest pathogens Armillaria and Heterobasidion;Evolutionary biology of the fungi , Korhonen, K.;Rayner, A.D.(et al.)(eds.) , / v.,pp.301-310, 1987
  15. Gastrodia elata and its symbiotic association with Armillaria mellea , Kusano, S. , Jour. Coll. Agric. / v.IV,pp.1-77, 1911
  16. Fundamentals of molecular evolution , Li, W.H.;Graur, D. , / v.,pp., 1991
  17. Nature of the black line produced between different biological species of Armillaria melle a complex , Mallet, K.I.;Hiratsuka, Y. , Can. J. Bot. / v.64,pp.2588-2590, 1986
  18. Identification of Kenyan Armillaria isolates by cultural morphology, intersterility tests and analysis of isozyme profiles , Mwangi, L.M.;Lin, D.;Hubbes, M. , Eur. J. For. Path. / v.19,pp.399-406, 1989
  19. Effect of concentration of $MgCl_2$ on Random Amplified DNA Polymorphism , Park, Y.H.;Kohel, R.J. , BioTechniques / v.16,pp.652-655, 1994
  20. NTSYS-pc, numerical taxonomy and multivariate analysis system , Rohlf, F.J. , / v.,pp., 1990
  21. Armillaria root disease. U.S.D.A For. Servo Agr. Handbook No.691 , Shaw, C.G. III;Kile, G.A. , / v.,pp., 1991
  22. The Agaricales in modern taxonomy , Singer, R. , / v.,pp.912, 1975
  23. Restriction fragment length polymorphisms in mitochondrial DNAs of Armillaria: identification of North American biological species , Smith, M.L.;Anderson, J.B. , Mycol. Res. / v.93,pp.247-256, 1989
  24. Studies on strain characteristics and identification of species using mating of Armillariella spp. , Sung, J.M;Cha, J.Y.;Yi, C.K.;Harrington, T.C. , Korean J. Plant Pathol. / v.6,pp.175-185, 1990
  25. Identification and culture of Armillaria mellea complex associated with root rot disease of Korean pine , Sung, J.M.;Cha, J.Y.;Kim, Y.S. , Korean J. Plant Pathol. / v.5,pp.317-327, 1989
  26. Biology and taxonomic concepts of Armillaria in Pinus forests in Korea , Sung, J.M.;Park, Y.J.;Kim, H.J.;Harrington, T.C. , Korean J. Plant Pathol. / v.7,pp.6-16, 1991
  27. Sex and diploidy in Armillaria mellea , Ullrich, R.C.;Anderson, J.B. , Experimental Mycology / v.2,pp.119-129, 1978
  28. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics;PCR protocols, a guide to methods and aplications , White, T.J.;Bruns, S.Lee;Taylor, J.;Innis, M.A.(ed.);Geland, D.H(ed.);Sninsky, J.J.(ed.);White, T.J.(ed.) , / v.,pp.315-322, 1990
  29. Genetic analysis with RAPD markers;More gene manipulations in fungi , Williams, J.G.K.;Kubelik, A,R,;Rafalski, J.A.;Tingey, S.V.;Bennett, J.W.(ed.);Lasure, L.L.(ed.) , / v.,pp.431-439, 1991
  30. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers , Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.;Tingey, S.V. , Nuc. Acids Res. / v.22,pp.6531-6535, 1990
  31. Association of random amplified polymorphic DNA markers with stromatal type in Hypoxylon truncatum sensu Miller , Yoon, C.S.;Glawe, D.A , Mycologia / v.85,pp.369-380, 1993
  32. A method for rapid small-scale preparation of fungal DNA , Yoon, C.S.;Glawe, D.A.;Shaw, P.D. , Mycologia / v.83,pp.835-838, 1991
  33. Optimization of the PCR program for RAPD analysis , Yu, K.;Pauls, K.P. , Nuc. Acids Res. / v.20,pp.2606, 1992

이 논문을 인용한 문헌 (1)

  1. Yoo, Young Bok ; Oh, Jin A ; Oh, Youn Lee ; Moon, Jiwon ; Shin, Pyung Gyun ; Jang, Kab Yeul ; Kong, Won-Sik 2013. "Investigation on ecological habitats in Armillaria gallica mushrooms" 한국버섯학회지 = Journal of mushroom science and production, 11(1): 36~40 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일