$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Isozyme Polymorphism 및 Random Amplified Polymorphic DNA(RAPD) Pattern에 의한 표고 버섯 품종간 비교

Differentiation of Lentinus edodes Isolates in Korea by Isozyme Polymorphisms and Random Amplified Polymorphic DNA (RAPD) Analysis

초록

Isozyme PAGE에 의한 isozyme polymorphism 및 random amplified polymorphic DNA(RAPD) pattern의 비교를 통하여 한국에서 수집된 63개의 표고 버섯(Lentinus edodes)품종의 유연관계를 조사하여 보았다. NTSYS PC program을 통하여 비교에 사용되어진 3가지 isozyme 즉 esterase, peroxidase, acid phophatase 등은 각각 10, 7, 3개의 isozyme polymorphism type으로 나뉘어질 수 있었고, 이러한 3개의 isozyme type들은 group-average method를 이용하여 최종적으로 6개의 집단으로 구분되어질 수 있었다. 또한 RAPD를 통한 표고 품종간 유연관계 비교는 사용되어진 총 20개의 random primer 들중 3개의 primer(OPA 03, OPA 18, OPA 19)가 품종간 polymorphism을 보였고 사용되어진 전체 random primer의 PCR product들의 집단 분석을 통해 최종적으로 5개의 집단으로 구분할 수 있었다. 사용되어진 두 가지 방법 즉, isozyme polymorphism및 RAPD pattern에 의해 구분되어진 63개 표고 버섯 품종의 subgroup level에서의 절대적인 유사성은 관찰할 수 없었다. Yii-Mattila(1996) 등에 의하면 품종간 비교시 isozyme analyses에서와는 달리 RAPD analyses에서는 많은 수의 strain-specific band들이 얻어질 수 있고 이것이 두 방법간의 유사성을 관찰하지 못하게 하는 원인이라고 보고하였다.

Abstract

Sixty-three isolates of Lentinus edodes obtained from Korea were used to assess the genetic similarity by isozyme polymorphisms and random amplified polymorphic DNA patterns. The activities of esterase, peroxidase and acid phosphatase displayed 10, 7 and 3 distinct isozyme patterns, respectively. By combining the isozyme patterns obtained with the 3 enzymes, every isolate showed its own distinct electrophoretic phenotypes. A distance matrix calculated between all pairs of 63 electrophoretic phenotypes based on the presence or abscence of isozyme bands were analyzed by the group-average method. Results of the cluster analysis assinged the 63 phenotypes into six major groups. In the analysis of random amplified polymorphic DNA patterns, all isolates of Lentinus edodes were devided into five RAPD groups.

저자의 다른 논문

참고문헌 (46)

  1. Linkage relationships of allozyme encoding loci in Lentinula edodes , Bowden, C.G.;Royse, D.J.;May, B. , Genome / v.34,pp.652-657, 1991
  2. A rapid and sensitive method for the quantitation of microgram quantities of protein , Bradford, M.M. , Analyt. Biochem. / v.72,pp.248, 1976
  3. The world production of cultivated edible mushroom in 1991 , Chang, S.T. , Mushroom J. Tropics / v.7,pp.117-120, 1992
  4. Virulence and isozyme patterns of Diaporthe phaseolorum var. sojae isolates from different geographic areas , Chin, M.S.;Kim, S.H.;Park, W.M. , RDA J. Agri. Science / v.35,pp.324-331, 1993
  5. Nutritional value;The biology and cultivation of edible mushroom , Crisan, ELI V.;Sands, A.;Chang, S.T.(ed.);Hayes, W.A.(ed.) , / v.,pp.137-168, 1978
  6. Isozyme variation and genetic relatedness in binucleate Rhizoctonia species , Damaj, M.;Jabaji-Hare, S.H.;Charesst, P.M. , Phytophatology / v.83,pp.864-871, 1993
  7. Inter- and intraspecific variation between population of Globodera rostochiensis and G. pallida revealed by random amplified polymorphic DNA , Folkertsma, R.T.;Rouppe van der Voort, J.N.A.M.;van Gent-Pelzer, M.P.E.;de Groot, K.E.;van den Bos, W.J.;Schots, A.;Bakker, J.;Gommers, F.J. , Phytopathology / v.84,pp.807-811, 1994
  8. Variation of isozyme patterns in the natural population of Lentinus edodes , Fukuda, M.;Tokimoto, K. , Proc. Jpn. Acad. / v.67,pp.43-47, 1991
  9. Electrophoretic pattern of soluble fungal proteins and their possible use as taxanomic creteria in genus Fusarium , Glynn, A.N.;Reid, J. , Can. J. Bot. / v.47,pp.1823-1831, 1969
  10. Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporium f. sp. pisi. , Grajai-Martin, M.J.;Simon, C.J.;Muehlbauer, F.J. , Phytophatology / v.83,pp.807-811, 1993
  11. Random amplified polymorphic DNA markers: A system for identifying and differentiating isolates of Colletotrichum graminicola , Guthrie, P.A.I.;Magill, C.W.;Frederiksen, R.A.;Odvody, G.N. , Phytopathology / v.82,pp.832-835, 1992
  12. Characterization of three Tremella species by isozyme analysis , Hanson, L.C.;Wells, K. , Mycologia / v.83,pp.446-454, 1991
  13. Use of random amplified polymorphic DNA makers for the detection of genetic variation in Magnaporthe poae , Huff, D.R.;Bunting, T.E.;Plumley, K.A. , Phytopathology / v.84,pp.1312-1316, 1994
  14. Vegetative compatibility, isozyme polymorphisms and pathogenicity of isolates of Fusarium oxysporum f. sp. fragariae , Hyun, J.W.;Park, W.M. , Kor. J. Plant Pathol. / v.12,pp.33-40, 1996
  15. Differntiation of Fusarium oxysporum f. sp. fragariae. isolates by random amplified polymorphic DNA (RAPD) analysis , Hyun, J.W.;Park, W.M. , Kor. J. Plant Pathol / v.12,pp.41-46, 1996
  16. Cultivation of Lentinus edodes;The biology of cultivation of edible mushrooms , Ito, T.;Chang, S.T.(ed.);Hayes, W.A.(ed.) , / v.,pp.461-473, 1978
  17. A comparative study on the protein and some isoenzyme patterns between races of the soybean cyst nematode (Heterodera glycines Ichinohe) , Lee, Y.B.;Park, W.M.;Paik, S.B. , Kor. J. Plant Pathol. / v.4,pp.49-53, 1988
  18. Use of RAPD technique for identification of Fusarium oxysporum f. sp. dianthi from carnation , Manulis, S.;Kogan, N.;Reuven, M.;Ben-Yephet, Y. , Phytopathology / v.84,pp.98-101, 1994
  19. Randomly amplified polymorphic DNA (RAPD) and isoenzyme analysis of Trypanosoma rangeli strains , Mario, S.;Emanuel, D.N.;Carlos, J.C.;Edmundo, C.G.;Carla, L.P.M.;Silvane, M.F.M.;Andrew, J.G.S.;Alvaro, J.R. , J. Euk. Microbiol. / v.41,pp.261-267, 1994
  20. Interspecific allozyme variation within the fungal genus Pleurotus , May, B.;Royse, D.J. , Trans. Br. Mycol. Soc. / v.90,pp.29-36, 1988
  21. Genetic variation in powdery mildew of barley: Development of RAPD, SCAR, and VNTR marker , McDermott, J.M.;Brandle, D.;Dutly, F.;Haemmerli, U.A.;Keller, S.;Muller, K.E.;Wolfe, M.S. , Phytopathology / v.84,pp.1316-1321, 1994
  22. Lentinuse and future , Miles, P.G.;Chang, S.T. , Mushroom News Letter for the Tropics / v.6,pp.2-3, 1985
  23. Kinoko no zosyoku to ikushu , Mutoh, S.;Nishi, S.(ed.) , / v.,pp.173-307, 1992
  24. Analysis of esterase and malate dehydrogenase isozymes of Lentinus edodes by isoelectric focusing for the identification and descrimination of stocks , Ohmasa, M.;Furukawa, H. , Trans. Mycol. Soc. Jpn. / v.27,pp.79-90, 1986
  25. Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification , Ouellet, T.;Seifert, K.A. , Phytopathology / v.83,pp.1003-1007, 1993
  26. Esterase isozyme of mycelium of Pyricularia oryzae under various cultural conditions , Park, S.H.;Park, W.M.;Kim, S.H.;Lee, J.E. , Kor. J. Plant Pathol. / v.3,pp.168-173, 1987
  27. Rice protein patterns. Comparision by various PAGE-technique in slabs , Park, W.M.;Stegemann, H. , J. Agronomy & Crop Science / v.148,pp.446-454, 1979
  28. Differentiation of physiologic races of the rice blast fungus, Pyricularia oryzae Cav. by PAGE-electrophoresis , Park, W.M.;Lee, Y.S.;Wolf, G.;Heitefuss, R. , J. Phytopathol. / v.117,pp.113-121, 1986
  29. Characterization of isolates of Ganoderma lucidum by electrophoretic patterns of enzymes , Park, W.M.;Lee, Y.S.;Kim, S.H.;Park, Y.H. , Kor. J. Mycology / v.14,pp.93-99, 1986
  30. Differentiation of Colletotrichum spp. causing anthracnose on Capsicum annum L. by electrophoretic method , Park, W.M.;Park, S.H.;Lee, Y.S.;Ko, Y.H.;Cho, E.K. , Kor. J. Plant Pathol. / v.3,pp.85-92, 1987
  31. A classification of the genus Lentinus Fr. , Pegler, D.N. , Kavaka / v.35,pp.36-41, 1975
  32. NTSYS-pc, numerical taxonomy and multivariate analysis system , Rohlf, F.J. , / v.,pp., 1990
  33. Identification of shitake genotypes by multilocus enzyme electrophoresis: catalog of lines , Royse, D.J.;May, B. , Biochem. Genet. / v.25,pp.705-716, 1987
  34. Single and joint segregation of marker loci in the shitake mushroom, Lentinus edodes , Royse, D.J.;Spear, M.C.;Mays, B. , J. Gen. App. Microbiol. / v.29,pp.217-222, 1983
  35. Cell line authentication and genetic relatedness of lines of the shitake mushroom, Lentinus edodes , Royse, D.J.;Spear, M.C.;Mays, B. , J. Gen. App. Microbio. / v.29,pp.205-216, 1983
  36. Methods of isozyme eletrophoresis for Trichoderma and Gliocladium species , Stasz, T.E.;Weeden, N.F.;Harman, G.E. , Mycologia / v.80,pp.870-874, 1988
  37. Random primer dependent PCR differentiates aggressive from nonaggressive isolates of the oliseed rape pathogen Phoma lingam (Lepto-spaeria maculans) , Schafer, C.;Wostemeyer, J. , J. Phytopathol. / v.136,pp.124-136, 1992
  38. Influence of Shitake (Lentinus edodes) on human serum cholesterol , Suzuki, S.;Oshima, S. , Mushroom Science / v.9,pp.463-467, 1976
  39. Biological nature of Lentinus edodes;The biology of cultivation of edible mushrooms , Tokimoto, K.;Komatsu, M.;Chang, S.T.(ed.);Hayes, W.A.(ed.) , / v.,pp.445-459, 1978
  40. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers , Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.;Tingey, S.V. , Nucleic Acid Res. / v.19,pp.6531-6535, 1990
  41. Characterisation of Fusarium oxysporum isolated from carnation in Australia based on pathogenecity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay , Wright, G.F.K.;Guest, D.I.;Wimalajeewa, D.L.S.;van Heeswijk, R. , European J. Plant Pathol. / v.102,pp.451-457, 1996
  42. A selective inhibitor of myxoviruses from shitake (Lentinus edodes) , Yamamura, Y.;Cochrane, K.W. , Mushroom Science / v.9,pp.495-507, 1976
  43. Isozyme and RAPD-PCR analyses of Fusarium avenaceum strains from Filand , Yii-Mattila, T.;Paavanen, S.;Hannukkala, A.;Parikka, P.;Tahvonen, R.;Karjalainen, R. , Plant Pathology / v.45,pp.126-134, 1996
  44. A method for rapid small-scale preparation of fungal DNA , Yoon, C.S.;Glawe, D.A.;Show, P.D. , Mycologia / v.83,pp.835-838, 1991
  45. Taxonomic relationships within the fungal Pleurotus ostreatus complex from the continental United States and adjacent Canada , Zervakis, G.;Labarere, J. , Can. J. Bot. / v.71,pp.113-128, 1992
  46. Genetic variability and systematics of eleven Pleurotus species based on isozyme analysis , Zervakis, G.;Sourdis, J.;Balis, C. , Mycol. Res. / v.98,pp.329-341, 1992

이 논문을 인용한 문헌 (1)

  1. 2004. "Genetic Variation of Korean Lepista nuda" 생명과학회지 = Journal of life science, 14(1): 115~120 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일