초록
1990년부터 1996년에 걸쳐 전국 35개 골프장에서 한지형잔디인 creeping bentgrass, Kentucky bluegrass 그리고 난지형잔디인 zoysiagyass의 잎마름 증상으로부터 Pythium spp. 125균주를 분리 하였고, 이를 동정한 결과 P. aphanidermatum, p. arrhenomanes, P. catenulatum, p. gramlnicola, p. myriotylum, P. oligandrum, P. periplocum, P. rostratum, P. torulosum, P. ultimum Trow var. ultimum 그리고 P. vanterpoolii로 모두 11개 종으로 동정되었다. 본 실험에서 사용한 semi-solid 상태의 'sucrose-asparagine bentgrass leaf culture' 방법을 이용할 경우 Pythium spp.의 동정에 필요한 포자낭, 장란기, 장정기 그리고 난포자의 형태적 특징의 관찰이 용이하였다. 동정된 Pythium spp.는 국내에서는 잔디류에 대하여 처음 보고되는 것이고, 이 중에서 P. arrhenomunes, P. cutenulatum, P. graminioota, P. oligandrum, P. periplocum, P. rostratum, P. torulosum 그리고 P. vanterpoolii는 국내에서 처음 보고되는 미기록종이다. P. myriotylum, P. rostratum, P. torulosum, P. vanterpoolii의 경우는 PCA 배지상에서 특이한 균사생장형을 나타내므로 이를 균 동정에 이용할 수 있었다.
Abstract
Eleven species of Pythium were identified from 125 isolates collected from leaf blight lesions on creeping bentgrass (Agrostis palustris Huds.), Kentucky bluegrass (Poa pratensis L.), and zoysiagrasses (Zoysia japonica Steud., and Z. matrella (L.) Merr.) at 35 golf courses in Korea in $1990{\sim}1996$. The identified species included P. aphanidermatum, P. arrhenomanes, P. catenulatum, P. graminicola, P. myriotylum, P. oligandrum, P. periplocum, P. rostratum, P. torulosum, P. ultimum var. ultimum, and P. vanterpoolii. Mycological characteristics of sporangia, oogonia, antheridia, and oospores observed on the sucrose-asparagine bentgrass leaf culture medium were described for each species. Of the species, P. arrhenomanes, P. catenulatum, P. gmminicola, P. oligandrum, P. periplocum, P. rostratum, P. torulosum and P. vanterpoolii were reported for the first time in Korea. P. myriotylum, P. rostratum, P. torulosum and P. vanterpoolii showed characteristic colony patterns on the potato-carrot agar medium, which can be used as criteria for species identification of Pythium.
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