초록

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반하의 조직배양을 통한 대량증식방법 확립의 일환으로 실시한 기내배양에서의 소괴경형성 및 식물체 분화에 영향을 미치는 배지 NAA와 Thidiazuron의 최적조건을 구명하고져 실시한 실험의 결과는 다음과 같다. 1, 식물생장조절물질로 NAA와 TDZ을 단독처리 할 때 TDZ $0.5\;{\mu}M$에서 shoot수가 45개로 가장 양호하였으며 root분화는 NAA 2.0 mg/l 에서 가장 양호하였다. 2. NAA 0.1 mg/l +TDZ $2.0\;{\mu}M$ 조합처리에서 shoot분화가 가장 양호하였으며 NAA 2.0 mg/l +TDZ $2.0\;{\mu}M$ 처리시에 가장 저조하였다. 3. 반하의 소괴경 형성은 MS배지에서는 TDZ $5.0\;{\mu}M$ 단독처리와 NAA 0.1 mg/l +TDZ $2.0\;{\mu}M$ 처리에서 소괴경 형성이 가장 양호하였다. B5배지에서는 TDZ $1.0\;{\mu}M$ 단독처리와 NAA 1.0 mg/l +TDZ $5.0\;{\mu}M$ 처리에서 소괴경 형성이 가장 양호하였으나 생체중은 NAA 0.1 mg/l 와 TDZ $5.0\;{\mu}M$의 단독처리에서 생체중이 가장 무거웠다. 4. MS, MG, B5배지조성에 따른 소괴경형성은 MG배지에서 30일배양후 가장 양호하였으며 생체중도 좋았다. 5. 분화된 식물체 뿌리분화에는 IAA보다 NAA가, MS 보다 1/2MS가 더 효과적인 결과를 보여 1/2MS배지에 NAA 2 mg/l 를 처리하였을 때 23.3개의 뿌리가 유도되어 가장 높은 결과를 보였다. 완전분화된 식물체를 vermiculite가 담긴 포트에 이식하여 온실에서 순화시킨 결과 80%정도의 생존율을 보였다.

Abstract ▼ AI-Helper

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combi...

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combination treatments of NAA and TDZ, as the level of thidiazuron increased, the rate of shoot regeneration was incresed while the increment of NAA concentration inhibited the rate of shoot regeneration. The supplement of $5\;{\mu}M$ thidiazuron produced the best number of micro-tubers per explant and the number of micro-tuber formed was 25 in MS medium and 29 in MG medium on 30 day culture, respectively. Microtuber formation was the best on MG medium with 1.0 mg/l NAA and $5\;{\mu}M$ thidiazuron. MG medium was superior to MS and B5 medium for the growth of tuberlets. Half strength of MS medium with NAA 2 mg/l was the most effective for root formation. Rooting ability on nursery soil of plantlets produced in in vjtro was good as a 80% after 3 weeks.