Kang, Jung-Sook
(Departments of Oral Biochemisrty and Molecular, College of Dentisty and Research Institute for Oral Biotechnology)
,
Kang, In-Goo
(Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan University)
,
Yun, Il
(Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan University)
We determined the microviscosity of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex and liposomes of total lipids (SPMTL) and phospholipids (SPMPL) extracted from SPMV. Changes in the microviscosity induced by the range and rate of lateral diffusion were measured by...
We determined the microviscosity of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex and liposomes of total lipids (SPMTL) and phospholipids (SPMPL) extracted from SPMV. Changes in the microviscosity induced by the range and rate of lateral diffusion were measured by the intramolecular excimerization of 1, 3-di(1-pyrenyl)propane (Py-3-Py). The microviscosity values of the direct probe environment in SPMV, SPMTL and SPMPL were 38.17, 31.11 and 27.64 cP, respectively, at$37^{\circ}C$and the activation energies $(E_a)$ of the excimer formation of Py-3-Py in SPMV, SPMTL and SPMPL were 8.236, 7.448 amd 7.025 kcal/mol, respectively. Probe location was measured by polarity and polarizability parameters of the probe Py-3-Py and probe analogues, pyrene, 1-pyrenenonanol and 1-pyrenemethyl-3${\beta}$-hydroxy-22, 23-bisnor-5-cholenate (PMC), incorporated into membranes or solubilized in reference solvents. There existed a good linear relationship between the first absorption peak of the $^1_a$ band and the polarizability parameter $(n^{2}-1)/(2n^{2}+1)$.The calculated refractive index values for SPMV, SPMTL and SPMPL were close to 1.50, which is higher than that of liquid paraffin (n=l.475). The probe location was also determined by using a polarity parameter $(f-1/2f^{I})$. Here f=$({\varepsilon}-1)/(2{\varepsilon}+1)$ is the dielectric constant function and $f^I=(n^2-1)/(2n^2+1)$ is the refractive index function. A correlation existed between the monomer fluorescence intensity ratio and the solvent polarity parameter. The probes incorporated in SPMV, SPMTL, and SPMPL report a polarity value close to that of 1-hexanol $({\varepsilon}=13.29)$. In conclusion, Py-3-Py is located completely inside the membrane, not in the very hydrophobic core, but displaced toward the polar head groups of phospholipid molecules, e.g., central methylene region of aliphatic chains of phospholipid molecules.
We determined the microviscosity of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex and liposomes of total lipids (SPMTL) and phospholipids (SPMPL) extracted from SPMV. Changes in the microviscosity induced by the range and rate of lateral diffusion were measured by the intramolecular excimerization of 1, 3-di(1-pyrenyl)propane (Py-3-Py). The microviscosity values of the direct probe environment in SPMV, SPMTL and SPMPL were 38.17, 31.11 and 27.64 cP, respectively, at$37^{\circ}C$and the activation energies $(E_a)$ of the excimer formation of Py-3-Py in SPMV, SPMTL and SPMPL were 8.236, 7.448 amd 7.025 kcal/mol, respectively. Probe location was measured by polarity and polarizability parameters of the probe Py-3-Py and probe analogues, pyrene, 1-pyrenenonanol and 1-pyrenemethyl-3${\beta}$-hydroxy-22, 23-bisnor-5-cholenate (PMC), incorporated into membranes or solubilized in reference solvents. There existed a good linear relationship between the first absorption peak of the $^1_a$ band and the polarizability parameter $(n^{2}-1)/(2n^{2}+1)$.The calculated refractive index values for SPMV, SPMTL and SPMPL were close to 1.50, which is higher than that of liquid paraffin (n=l.475). The probe location was also determined by using a polarity parameter $(f-1/2f^{I})$. Here f=$({\varepsilon}-1)/(2{\varepsilon}+1)$ is the dielectric constant function and $f^I=(n^2-1)/(2n^2+1)$ is the refractive index function. A correlation existed between the monomer fluorescence intensity ratio and the solvent polarity parameter. The probes incorporated in SPMV, SPMTL, and SPMPL report a polarity value close to that of 1-hexanol $({\varepsilon}=13.29)$. In conclusion, Py-3-Py is located completely inside the membrane, not in the very hydrophobic core, but displaced toward the polar head groups of phospholipid molecules, e.g., central methylene region of aliphatic chains of phospholipid molecules.
J. Biol. Chem. G. R. Barlett 234 466 1959 10.1016/S0021-9258(18)70226-3 Barlett, G. R.: Phosphorus assay in column chromatography.J. Biol. Chem., 234, 466-468 (1959).
Arch. Pharm. Res. I. K. Chung 16 191 1993 10.1007/BF02974481 Chung, I. K., Kang, J. S. and Yun, I.: Effects ofn-alkanols on the lateral diffusion of total phospholipid fration extracted from brain membranes.Arch. Pharm. Res., 16, 191-195 (1993).
J. Am. Chem. Soc. K. Kalyanasundaram 99 2039 1973 10.1021/ja00449a004 Kalyanasundaram, K. and Thomas, J. K.: Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their application in studies of micellar systems.J. Am. Chem. Soc., 99, 2039-2044 (1973).
Biochim. Biophys. Acta J. S. Kang 1281 157 1996 10.1016/0005-2736(95)00301-0 Kang, J. S., Choi, C. M. and Yun, I.: Effect of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mouse myeloma cell line Sp2/0-Ag14.Biochim. Biophys. Acta, 1281, 157-163 (1996).
Arch. Pharm. Res. J. S. Kang 15 196 1992 10.1007/BF02974053 Kang, J. S., Chung, Y. Z., Cho, G. J., Byun, W. T. and Yun, I.: Membrane-ordering effects of barbiturates on pure phospholipid model membranes.Arch. Pharm. Res., 15, 196-203 (1992).
Asia Pacific J. Pharmacol. J. S. Kang 9 67 1994 Kang, J. S. and Yun, I.: Effects of lindane on microviscosity of brain membranes.Asia Pacific J. Pharmacol., 9, 67-71 (1994).
Cienc. Biol. (Coimbra) V. M. C. Madeira 2 265 1976 Madeira, V. M. C. and Antunes-Maderira, M. C.: Lipid composition of biomembranes: a complete analysis of sarcoplasmic reticulum phospholipids.Cienc. Biol. (Coimbra), 2, 265-291 (1976).
Biophys. J. R. L. Melnick 34 499 1981 10.1016/S0006-3495(81)84864-3 Melnick, R. L., Haspel, H. C., Goldenberg, M., Greenbaum, L. M. and Weinstein, S.: Use of fluorescent probes that form intramolecular excimers to monitor structural changes in model and biological membranes.Biophys. J., 34, 499-515 (1981).
Bull. Chem. Soc. Japan A. Nakajima 46 2602 1973 10.1246/bcsj.46.2602 Nakajima, A.: Fluorescence lifetime of pyrene in different solvents.Bull. Chem. Soc. Japan, 46, 2602-2604 (1973).
J. Chem. Phys. J. R. Platt 17 484 1949 10.1063/1.1747293 Platt, J. R.: Classification of spectra of cata-condensed hydrocarbons.J. Chem. Phys., 17, 484-495 (1949).
Mol. Cells I. Yun 1 15 1990 10.1016/S1016-8478(23)13825-8 Yun, I. and Kang, J. S.: The general lipid composition and aminophospholipid asymmetry of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex.Mol. Cells, 1, 15-20 (1990).
Arch. Pharm. Res. I. Yun 13 325 1990 10.1007/BF02858167 Yun, I., Kim, Y. S., Yu, S. H., Chung, I. K., Kim, I. S., Baik, S. W., Cho, G. J., Chung, Y. Z., Kim, S. H. and Kang, J. S.: Comparison of several procedures for the preparation of synaptosomal plasma membrane vesicles.Arch. Pharm. Res., 13, 325-329 (1990).
J. Membrame Biol. I. Yun 138 221 1994 Yun, I., Lee, S. H. and Kang, J. S.: The effect of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured Mar 18.5 hybridoma cells.J. Membrame Biol., 138, 221-227 (1994).
Chem. Phys. Lett. K. A. Zachariasse 73 6 1980 10.1016/0009-2614(80)85190-6 Zachariasse, K. A., Kohnle, W. and Weller, A.: Intramolecular excimer fluorescence as a probe of fluidity changes and phase transitions in phosphatidylcholine bilayers.Chem. Phys. Lett., 73, 6-11 (1980).
※ AI-Helper는 부적절한 답변을 할 수 있습니다.