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제안 방법
- The objective of the present study was to subdivide the lysine requirements into growth and maintenance, and to compare the estimates to previous estimates of total lysine requirements.
이론/모형
- All parameters were determined at the point at which the residual sum of squares was minimized by the Nonlinear Least Squares method (SAS, 1985). The equation covering the plateau portion was not included in the equation estimating lysine requirement because, genetic potential, energy or some nutrients other than amino acids were limiting response in that region of the response curve (figure 1).
- After removing intestinal contents, carcasses including internal organs and feathers were stored at -20 ℃ until body composition (total body water and N content) was determined. Carcass samples were freeze dried (Ilsin Engineering, Korea), ground and analyzed by AOAC (1990) methods. At the beginning of the experiment, body composition of 6 chicks (8 days old) was determined for calculation of body nitrogen.
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