초록

단감이 경제과수로 점차 부각되면서 재배면적이 증가하는 반면 단일 품종으로 편중됨에 따라 주요 재배지를 중심으로 그간 문제시되지 않았던 병해가 발생하여 이로 인해 단감의 품질과 농가소득에 많은 손해를 초래하고 있다. 이중 중요 병해는 Pestalotiopsis theae에 의한 단감나무 둥근갈색무늬병을 들 수 있고, 아직까지 국내에서는 Pestalotiopsis theae에 대한 구체적인 연구가 수행되지 않고 있는 실정이며, 본 연구는 이들 병원균에 대한 기초정보를 밝히기 위하여 수행되었다. Random amplified polymorphic DNA(RAPD)를 사용하여 P. theae의 유전적 유연관계를 분석하였다. P-28(No. 14)과 SP-33 (No. 15)은 0.976으로 97%의 가장 높은 유사성을 나타났으며 SP-18 (No. 7)과 SP-21 (No. 9)은 0.430으로 가장 낮은 유사성을 보였다. 서로 비교한 균주들 간의 유사성은 60% 이하로부터 95% 이상인 것도 있었으며, 대다수의 균주들은 $50{\sim}80%$의 유사성을 나타내었다. SP-21 (No.9)은 고립된 한 집단으로 나타났으며 비교한 모든 균주와 60% 이하의 낮은 유사성을 나타내었다.

Abstract

In this study, we evaluated the genetic relationships of fourty seven Pestalotiopsis theae isolates collected from diseased sweet persimon in various places in southern part of Korea using RAPD (Randomly Amplified Polymorphic DNAs) method. As a result of the amplification, eight primers showed total of 86 bands ranging from 0.3 Kb to 3.2 Kb. Among those 86 bands, 84 polymorphic bands were used for bionominal matrix code (0, 1), and UPGMA dendrogram analysis. Similarities among the compared isolates ranged from below 60% to more than 95%. Most of the compared isolates showed $50{\sim}80%$ similarities. The number of isolate pairs which showed more than 80% similarity were 248. The number of isolate pairs which showed $50{\sim}80%$ similarity were 789, and the number of isolate pairs which showed below 50% similarity were 21. Isolate SP-21 (No.9) showed below 50% similarity with all the isolates compared. At 50% similarity level, all the isolates compared, except isolate SP-21 (No.9), were included in one big group. At 65% similarity level, all the isolates compared, except isolate SP-21 (No.9), were divided into three different groups. At 75% similarity level, all the isolates compared, except isolates SP-47 (No. 23) and SP-21 (No.9), were divided into six different groups.

주제어

#Pestalotiopsis theae   #RAPD method  

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참고문헌 (20)

1. 단감재배 신기술 , 김선봉 , / v.,pp.103, 1992
   
2. Studies on cultural characteristic of Pestalotiopsis theae causing leaf blight on oriental persimmon tree , Chang, T.H.;Lim, T.L.;Chung, B.K.;Kim, B.S. , Korean J. Plant Pathology / v.13,pp.232-238, 1997
   
3. Occurrence of leaf blight on sweet persimmon tree by Pestalotiopsis theae , Chang, T.H.;Lim, T.L.;Chung, B.K.;Kim, B.S.;Shim, H.K. , Korean J. Plant Pathology / v.12,pp.377-379, 1996
   
4. Evaluation of genetic diversity among the Fusarium oxysporum and their formae specialis from various sources , Choi, H.S.;Kim, K.S.;Shim, J.O.;Kim, B.S.;Lee, M.W.;Lee, Y.S. , J. Agric. Research / v.8,pp.29-36, 1997
   
5. RAPD analysis for the evaluation of genetic diversity among the Fusarium species from various sources , Choi, H.S.;Kim, K.S.;Kim, M.J.;Shim, J.O.;Kim, B.S.;Lee, M.W.;Lee, Y.S. , Korean J. Mycology / v.25,pp.202-208, 1997
   
6. Differentiation of Fusarium solani f. sp. cucurbitae race 1 and 2 by random amplification of polymorphic DNA , Crowhurst, R.N.;Hawthorne, B.T.;Rikkerink, E.H.A.;Templeton, M.D. , Curr. Genet. / v.20,pp.391-396, 1991
   
7. Monograph of the genus Pestalotia Part I , Guba, E.F. , Phytopathology / v.19,pp.191-233, 1929
   
8. Monograph of the genus Pestalotia Part II , Guba, E.F. , Mycologia / v.24,pp.355-397, 1932
   
9. Random amplified polymorphic DNA markers: A system for identifYing and differentiating isolates of Colletotrichum graminicola , Guthrie, P.A.I.;Magill, C.W.;Frederiksen, R.A.;Odvody, G.N. , Phytopathology / v.82,pp.823-835, 1992
   
10. Detection of Xanthomonas campestris pV. citri by the polymerase chain reaction method , Hartung, J.S.;Daniel, J.F.;Pruvost, O.P. , Appl. Environ. Microbiol. / v.59,pp.1143-1148, 1993
    
11. Randomly amplified polymorphic DNA (RAPD) profiling of insect parasitic fungi including Cordyceps species , Kim, K.S.;Choi, H.S.;Lee, M.W.;Shim, J.W.;Lee, Y.S. , J. Agric. Science / v.8,pp.23-28, 1997
    
12. Genetic diversity of leaf blight pathogen of sweet persimmon Pestalotiopsis species with random amplified polymorphic DNA (RAPD) , Kim, K.S.;Choi, H.S.;Lim, T.H.;Chang, T.H.;Chung, B.K.;Kim, M.J.;Lee, Y.S. , Korean J. Plant Pathology / v.13,pp.311-316, 1997
    
13. Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification , Ouekket, F.;Seifert, K.A. , Phytopathology / v.83,pp.1003-1007, 1993
    
14. Extraction of DNA from plant tissue , Rogers, S.O.;Bendich, A.J. , Plant Mol. Biol. Man. / v.6,pp.1-10, 1988
    
15. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. (Version 1. 80), Computer program distributed by Exeter Software , Rohlf, F.J. , / v.,pp., 1993
    
16. Cluster Analysis for Researchers , Romesburg, H.C. , / v.,pp., 1984
    
17. Characterization of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis , Smith, J.J.;Scott-Craig, J.S.;Leadbetter, J.R.;Bush, G.L.;Roberts, D.L.;Fulbright, D.W. , Mol. Phylogent. Evol. / v.3,pp.135-145, 1994
    
18. Numerical Taxonomy , Sneath, P.H.;Sokal, R.R. , / v.,pp., 1973
    
19. New and old species of Pestalotiopsis , Steyaert, R.L. , Transactions of British. Mycological Society / v.36,pp.81-89, 1953
    
20. DNA polymorphism amplified by arbitary primers are useful as genetic markers , Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.;Tingey, S.V. , Nucleic Acids Res. / v.18,pp.6531-6535, 1990
    

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