$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

Simple procedures have been devised for purifying recombinant human interleukin-2 (hIL-2), which was expressed in Escberichia coli using sequences of glucagon molecules and enterokinase cleavage site as an N-terminus fusion partner. The insoluble aggregates of recombinant fusion protein produced in E. coli cytoplasm were easily dissolved by simple alkaline pH shift $(8\rightarrow12\rightarrow8)$. Following enterokinase cleavage, the recombinant hIL-2 was finally purified by one-step reversed-phase HPLC with high purity. The ease and high efficiency of this simple purification process seem to mainly result from the role of used glucagon fusion partner, which could be applied to the production of other therapeutically important proteins.

참고문헌 (11)

  1. Interleukin-2 augments natural killer cell activity , Henney, C. S.;K. Kuribayashi;D. E. Kern;S. Gillis , Nature / v.291,pp.335-338, 1981
  2. Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2 , Farrar, W. L.;H. M. Johnson;J. J. Farrar , J. Immunol. / v.126,pp.1120-1125, 1981
  3. Human immunodeficiency virus (HIV) type-1 GP120-specific cell-mediated cytotoxicity (CMC) and natural killer (NK) activity in HIV-infected (HIV+) subjects: enhancement with interleukin-2 (IL-2), IL-12, and IL-15. , Lin, S. J.;R. L. Roberts;B. J. Ank;O. H. Nguyen;E. K. Thomas;E. R. Stiehm , Clin. Immunol. Immunopathol. / v.82,pp.163-173, 1997
  4. Characterization of human interleukin 2 derived from Escherichia coli , Liang, S. M.;B. Allet;K. Rose;M. Hirschi;C. M. Liang;D. R. Thatcher , Biochem. J. / v.229,pp.429-439, 1985
  5. Glucagon-induced self-association of recombinant proteins in Escherichia coli and affinity purification using a fragment of glucagon receptor , Kim, K.-Y.;J. Lee;V. Saraswat;Y. -H. Park , Manuscript submitted to Biotechnol. Bioeng / v.,pp., 0000
  6. Purification and renaturation of recombinant human interleukin-2 , Weir, M. P.;J. Sparks , Biochem. J. / v.245,pp.85-91, 1987
  7. Purification and characterization of recombinant human interleukin-2 produced in Escherichia coli , Kato, K.;T. Yamada;K. Kawahara;H. Onda;T. Asano;H. Sugino;A. Kakinuma , Biochem. Biophys. Res. Comm. / v.130,pp.692-699, 1985
  8. Preparation of peptides by use of glucagon and its variants as a fusion expression partner , Park, Y. -H.;J. Lee;D. -Y. Kim , Korea Patent Application / v.,pp.7641, 1999
  9. Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level , Saraswat, V.;D. -Y. Kim;J. Lee;Y. -H. Park , FEMS Microbiol. Lett. / v.179,pp.367-373, 1999
  10. Characterization of disulfide bonds in recombinant proteins: redeced human interleukin 2 in inclusion bodies and its oxidative refolding , Tsuji; T.;R. Nakagawa;N. Sugimoto;K. Fukuhara , Biochemistry / v.26,pp.129-134, 1987
  11. Disulfide scrambling of in terleukin-2: HPLC resolution of the three possible isomers , Browning, J. L.;R. J. Mattaliano;E. P. Chow;S. M. Liang;B. Allet;J. Rosa;J. E. Smart , Anal. Biochem. / v.155,pp.123-128, 1986

이 논문을 인용한 문헌 (1)

  1. 2000. "Study on Proteolysis of Glucagon .3-Interleukin-2 {G.3-IL-2} Using Enterokinase" 한국생물공학회지 = Korean journal of biotechnology and bioengineering, 15(3): 238~242 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일