• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Chiral Separation of Tryptophan Enantiomers by Liquid Chromatography with BSA-Silica Stationary Phase


The separation of tryptophan enantiomers was carried out with medium-pressure liquid chromatography using BSA (bovine serum albumin)-bonded silica as a chiral stationary phase. The influence of various experimental factors such as pH and ionic strength of mobile phase, separation temperature, and the presence of organic additives on the resolution was studied. In order to expand this system to preparative scale, the loadability of sample and the stability of stationary phase for repeated use were also examined. The separation of tryptophan enantiomers was successful with this system. The data indicated that a higher separation factor (a) was obtained at a higher pH and lower temperature and ionic strength in mobile phase. Addition of organic additives (acetonitrile and 2-propanol) in mobile phase contributed to reduce the retention time of L-tryptophan. About $30\%$ of the separation factor was reduced after 80 days of repeated use.

참고문헌 (20)

  1. Commercially available brush-type chiral selectors for direct resolution of enantiomers , Perrin, S. P.;W. H. Pirkle , ACS Symp. Ser. / v.471,pp.43-66, 1991
  2. Resolution of race-mic drugs on a new chiral column based on silica-immo-bilized cellobiohydrolase , Hermansson, J.;A. Grahn , J. Chromatogr. / v.A687,pp.45-59, 1994
  3. Synthesis and chromatographic properties of an HPLC stationary phase based upon human serum albumin , Domenici, E.;C. Bertucci;P. Salvadori;G. Felix;I. Cahabne;S. Motellier;I. Wainer , Chromatographia / v.29,pp.170-176, 1990
  4. High-performance liquid affinity chromatography , Larsson, P. O. , Methods Enzymol / v.104,pp.212-223, 1984
  5. Protein column-based chiral reversed-phase liquid chromatography , Allenmark, S. G. , ACS Symp. Ser. / v.471,pp.114-125, 1991
  6. A quantitative relationship between capacity factor and selector concentration in capillary electrophoresis and high performance liquid chromatography , Lloyd, D. K. , Electrophoresis / v.18,pp.958-964, 1997
  7. Enantioselectivety of complex formation in ligand-exchange chromatographic systems with chiral stationary phase and/or chiral mobile phase , Davankov, V. A.;A. Kurganov;T. M. Ponomareva , J. Chromatogr / v.452,pp.309-316, 1988
  8. Chromatographic optical resolution on polysaccharide carbamate phases , Okamoto, Y.;Y. Kaida;R. Aburatani;K. Hatada , ACS Symp. Ser. / v.471,pp.101-113, 1991
  9. A chiral separation: An overview , Ahuja, S. , ACS Symp. Ser. / v.471,pp.1-26, 1991
  10. Direct liquid chromatographic resolution of racemic drugs using α₁-acid glycoprotein as the chiral stationary phase , Hermansson, J. , J. Chromatogr. / v.269,pp.71-80, 1983
  11. Direct liquid chromatographic separation of enantiomers on immobilozed protein stationary phases , Allenmark, S.;B. Bomgren;H. Boren , J. Chromatogr. / v.264,pp.63-68, 1983
  12. Direct analytical and preparative resolution of enantiomers using albumin adsorbed to silica as a stationary phase , Erlandsson, P.;L. Hansson;R. Isaksson , J. Chromatogr. / v.370,pp.470-483, 1986
  13. Retention data from affinity high-performance liquid chromatography in view of chemometrices , Kaliszan, R. , J. Chromatogr. / v.B715,pp.229-244, 1998
  14. Protein chiral selectors in free-solution capillary electrophoresis and packed-capillary electrochromatography , Lloyd, D. K.;S. Li;P. Ryan , J. Chromatogr. / v.A694,pp.285-296, 1995
  15. Chromatography on chiral stationary phase , Allenmark, S.;V. Schrig , J. Mater. Chem. / v.7,pp.1955-1963, 1997
  16. Enantiomeric impurities in chiral catalysts, auxiliaries and synthons used in enantioselective synthesis , Armstrong, D. W.;J. T. Lee;L. W. Chang , Tetrahedron: Asymmetry / v.9,pp.2043-2064, 1998
  17. Development of dihydrazide-activated silica supports for high-performance affinity chromatography , Ruhn, P. F.;S. Garver;D. S. Hage , J. Chromatogr. / v.A669,pp.9-19, 1994
  18. Preparative chromatographic separation of enantiomers , Francotte, E.;A. Junker-Buchheit , J. Chromatogr. / v.576,pp.1-45, 1992
  19. Separation procedures used to reveal and follow drug-protein binding , Sebille, B.;R. Zini;C. V. Madjar;N. Thuaud;J. P. Tillement , J. Chromatogr. / v.531,pp.51-77, 1990
  20. Chiral separations in capillary electro-phoresis using proteins as stereoselective binding agents , Hage, D. , Electrophoresis / v.18,pp.2311-2321, 1997

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일