[논문]Identification of Genetic Markers for Korean Native Cattle (Hanwoo) by RAPD Analysis

Yeo Jung Sou; Lee Ji Sun; Lee Chang Hee; Jung Young Ja; Nam Doo Hyun

doi:10.1007/bf02932348

Identification of Genetic Markers for Korean Native Cattle (Hanwoo) by RAPD Analysis 원문보기

Biotechnology and bioprocess engineering : Bbe, v.5 no.1, 2000년, pp.23 - 26

Yeo Jung Sou (Department of Animal Sciences, College of Natural Resources, Yeungnam University) , Lee Ji Sun (Division of Pharmacy, College of Pharmacy, Yeungnam University) , Lee Chang Hee (Division of Pharmacy, College of Pharmacy, Yeungnam University) , Jung Young Ja (Korea Food and Drug Administration) , Nam Doo Hyun (Division of Pharmacy, College of Pharmacy, Yeungnam University)

Abstract ▼ AI-Helper

In order to develop the specific genetic marker for Korean native cattle (Hanwoo), randomly amplified polymorphic DNA (RAPD) analysis of 6 different cattle breeds was attempted by using 38 decamer primers. In comparison of RAPD patterns, two distinctive DNA bands specific for Hanwoo were detected. One was 296 bp of DNA fragment found to be specific only for female Hanwoo when primer GTCCACACGG was employed. In individual analysis of this RAPD marker was observed only in female individuals with the possibility of $85.3\%$. The other was 521 bp of RAPD marker amplified using TCGGCGATAG and AGCCAGCGAA primers, which showed $83.0\%$ of genetic frequency in 85 male and 68 female individuals tested. Nucleotide sequencing of these genetic markers revealed that 296 bp marker has a short micro satellite-like sequence, ACCACCACAC, and a tandem repeat sequence of microsatellite GAAAAATG in the determined sequence. Two distinctive tandem repeats of microsatellite sequences, MC and GAAGA, were also appeared in 521 bp DNA marker. In BLAST search, any gene having high homology with these markers was not found.

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문제 정의

In this study, identification of new genetic markers more specific in Korean native cattle (Hanwoo) by analyzing the DNA polymorphisms by RAPD method, which are not detected in the other imported breeds in Korea. The identified genetic markers will be applied in the identification of Hanwoo and further the evaluation of their specific genetic traits.
Acknowledgement The work was kindly supported by the Agricultural Research Promotion Center as Hi-Tech agricultural and forestry research grant (1998). The authors give their thanks to Dr. Young Boon Yang in Cheju National University for his kind supply of the blood samples for foreign imported cattles.

제안 방법

In RAPD analysis, primers with ten nucleotides and a (G + C) content of at least 50% are generally employed, because primers having high (A+T) content may cause DNA-primer hybrid melted during polymerization at 72℃ [14]. In this study, 38 different decamer primers have been employed for the analysis of DNA polymorphism of Hanwoo and other cattle breeds (Angus, Brahman, Charolais, Holstein, and Simmental) by RAPD procedure (Table 1). Those primers were subjected as a single primer or in the combination with other primers.

대상 데이터

The blood samples of Hanwoo were kindly supplied from Korean Federation of Animal Improvement (Seoul, Korea), and those of Holstein were taken in Animal Farm of Yeungnam University (Kyongsan, Korea). White blood cells collected from 10 mL of blood samples were lysed by SDS and proteinase K, and the genomic DNAs were extracted by phenol-chloroform method [13].

성능/효과

In order to confirm whether genetic markers found by RAPD analysis are the specific genetic markers only for Hanwoo, the genomic DNAs of 153 individuals consisting of 85 male and 68 female were subjected to RAPD amplification. As seen in Table 2, 85.
It can be concluded that the genetic markers identified by RAPD analysis of DNA polymorphism in this study are more specific and more reproducible for Hanwoo. Furthermore, these results suggest that RAPD analysis procedure is a powerful tool for identification of genetic markers specific to Hanwoo, compared with other cattle breeds.

참고문헌 (16)

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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