[논문]Characterization of Sulfur Oxidation by an Autotrophic Sulfur Oxidizer, Thiobacillus sp. ASWW-2

Lee Eun Yaung; Cho Kyung-Suk; Ryu Hee Wook

doi:10.1007/bf02932353

Characterization of Sulfur Oxidation by an Autotrophic Sulfur Oxidizer, Thiobacillus sp. ASWW-2 원문보기

Biotechnology and bioprocess engineering : Bbe, v.5 no.1, 2000년, pp.48 - 52

Lee Eun Yaung (Department of Environmental Science and Engineering, Ewha Womans University) , Cho Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University) , Ryu Hee Wook (Department of Chemical and Environmental Engineering, Soong Sil University)

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An autotrophic sulfur oxidizer, Thiobacillus sp. ASWW-2, was isolated from activated sludge, and its sulfur oxidation activity was characterized. Thiobacillus sp. ASWW-2 could oxidize elemental sulfur on the broad range from pH 2 to 8. When 5-50 g/L of elemental sulfur was supplemented as a substrate, the growth and sulfur oxidation activity of Thiobacillus sp. ASWW-2 was not inhibited. The specific sulfur oxidation rate of strain ASWW-2 decreased gradually until sulfate was accumulated in medium up to 10 g/L. In the range of sulfate concentration from 10 g/L to 50 g/L, the sulfur oxidation rate could keep over $2.0g-S/g-DCW{\cdot}d$. It indicated that Thiobacillus sp. ASWW-2 has tolerance to high concentration of sulfate.

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The flasks were charged with 100 mL of the basal mineral medium. To investigate the effect of initial pH on sulfur oxidation rate, 10 g/L of S° was supplemented in the medium as a substrate, and the initial pH of the medium was adjusted to 1, 2, 3, 4, 5, 6, 7, 8 with 2N NaOH or 2N HCl. Also, the effects of substrate concentration (5-50 g/L of S° and 1.

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). IC-Pak^TM anion column (4.6 mm Φ × 50 mm L, Waters, U.S.A.) and conductivity detector (Waters 432, U.S.A.) were used.

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