[논문]Screening of Microorganisms Producing Esterase for the Production of <tex>$(R)-\\beta-Acetylmercaptoisobutyric$</tex> Acid from Methyl <tex>$(R,S)-\\beta-Acetylmercaptoisobutyrate$</tex>

Gokul Boyapati; Lee Je-Hyuk; Song Ki-Bang; Panda T.; Rhee Sang-Ki; Kim Chul-Ho

doi:10.1007/bf02932355

Screening of Microorganisms Producing Esterase for the Production of $(R)-\\beta-Acetylmercaptoisobutyric$ Acid from Methyl $(R,S)-\\beta-Acetylmercaptoisobutyrate$ 원문보기

Biotechnology and bioprocess engineering : Bbe, v.5 no.1, 2000년, pp.57 - 60

Gokul Boyapati (Biotechnology Research Centre, Indian Institute of Technology) , Lee Je-Hyuk (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) , Song Ki-Bang (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) , Panda T. (Biotechnology Research Centre, Indian Institute of Technology) , Rhee Sang-Ki (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) , Kim Chul-Ho (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology)

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$(R)-\beta-acetylmercaptoisobutyric$ acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting $(R,S)-\beta-acetylmercaptoisobutyric$ acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain of Pseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to $70^{\circ}C$. This strain could produce RAM asymmetrically from (R,S)-ester.

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The strains from Korean Collection for Type Cultures (KCTC) and Agricultural Research Service (ARS Collection in United States Department of Agriculture), also referred to as the NRRL Culture Collection, were tested for the comparison of RAM-production with IS 1001. The identification of IS 1001 was been done by Korean Collection for "Type Cultures (KCTC). A comparison of amount of RAM produced by IS 1001 was made with other strains with α-alkylcarboxylic acid and alkyl esterhydrolyzing enzyme and is given in table 1.
The hydrolyzed products and RAM production were determined by gas chromatography. The analysis was done using SUPELCOWAX-10 column, 30 m × 0.53 mm, 0.50 µm film (Supelco, U.S.A.). Analysis conditions for the GC were; oven temperature: 100℃; injector and detector temperature: 170℃ and 180℃, respectively; the flow rate of the N₂ carrier gas: 20 m³/min.

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Science M. A. Ondetti 196 441 1977 10.1126/science.191908 Ondetti, M. A., B. Rubin, and D.W. Cushman (1977) Design of specific inhibitors of angiotensin-converting enzyme: New class of orally active antihypertensive agents.Science 196: 441-444.

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Samayama, T., H. Kinugasa, H. Nishimura, K. Takeyama, and K. Hosoki (1988)Japan Patent 63-18599.
Biochem. D. W. Cushman 16 5484 1977 10.1021/bi00644a014 Cushman, D. W., H. S. Cheung, E. F. Sabo, and M. A. Ondetti (1977) Design of potent competitive inhibitors of angiotensin-converting enzyme. Carboxyalkanoyl and mereapotoalkanoyl amino acids.Biochem. 16: 5484-5491. Koho, 80-38386.

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Iwao J., M. Oya, E. Kato, and T. Watanabe (1978) Japan Kokai Tokyo Koho, 78-151912.
Ohashi, N., S. Nagata, and S. Katsube (1981) Japan Kokai Tokyo Koho, 81-7756.
Biosci. Biotech. Biochem. S. Akihiro 57 17 1993 10.1271/bbb.57.17 Akihiro, S., K. Yoshimasa, N. Ohsuga, R. Numazawa, and H. Ohnishi (1993) Chemical racemization of methyl L-β-acetylthioisobutyrate.Biosci. Biotech. Biochem. 57: 17-19.

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JAOCS R. N. Patel 72 1247 1995 10.1007/BF02546196 Patel, R. N., B. Amit, and J. S. Laszlo (1995) Synthesis of four chiral pharmaceutical intermediates by biocatalysis.JAOCS 72: 1247-1264.

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Biosci. Biotech. Biochem. S. Akihiro 56 1252 1992 10.1271/bbb.56.1252 Akihiro, S., H. Akihiko, K. Etsuko, O. Naoto, N. Ryozo, W. Ichiro, and O. Hisoa (1992) Screening of microorganisms producing D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate.Biosci. Biotech. Biochem. 56: 1252-1256.

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J. Am. Chem. Soc. C.-S. Chen 103 3580 1931 10.1021/ja00402a059 Chen, C.-S., Y. Fujimoto, and C. J. Shi (1931) Bifunctional chiral synthons via microbiological methods. 1. Optically active 2,4-dimethylglutaric acid monomethyl esteres.J. Am. Chem. Soc. 103: 3580-3582.

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Agric. Biol. Chem. S. Iriuchijima 45 1389 1981 10.1271/bbb1961.45.1389 Iriuchijima, S. and A. Keiyu (1981) Asymmetric hydrolysis of (±)-α-substituted carboxylic acid erters with microorganisms.Agric. Biol. Chem. 45: 1389-1392.

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Biosci. Biotech. Biochem. S. Akihiro 57 782 1993 10.1271/bbb.57.782 Akihiro, S., O. Eiji, T. Hiroko, O. Naoto, N. Ryozo, M. Itsumi, H. Eiichi, and O. Hisao (1993) Process conditions for production of D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate with the cells ofPseudomonas putida MR-2068.Biosci. Biotech. Biochem. 57: 782-786.

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