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Abstract

The fungus, Emericellopsis minima W2, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from a waste water sample. Production of the PHB depolymerase from E. minima W2 (PhaZ/ sub Emi/) was significantly repressed in the presence of glucose. PhaZ/ sub Emi/ was purified by column chromatography on Octyl-Sepharose CL-4B and Sephadex G-100. The molecular mass of the PhaZ/ sub Emi/), which consisted of a single polypeptide chain, was estimated to be 48.0 kDa by SDS-PAGE and its pI vague was 4.4. The maximum activity of the PhaZ/ sub Emi/ was observed at pH 9.0 and 55$\^{C}$. It was significantly inactivated by 1mM dithiothreitol, 2mM diisopropyl fluorphosphate, 0.1mM Tween 80, and 0.1 mM Triton X-l00, but insensitive to phenylmethylsulfonyl fluoride and N-ethylmaleimide. The PhaZ/ sub Emi/ efficiently hydrolyzed PHB and its copolyester with 30 mol% 3-hydroxyvalerate, but did not act on poly(3-hydroxyoctanoate). It also hydrolyzed p-nitrophenylacetate and p-nitrophenylbutyrate but hardly affected the longer-chain forms. The main hydrolysis product of PHB was identified as a dimer of 3-hydroxybutyrate.

참고문헌 (19)

  1. Effect of levulinic acid on the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)by Ralstonia eutropha KHB-8862 , Chung, S.H.;G.G. Choi;H.W. Kim;Y.H. Rhee , J. Microbiol. / v.39,pp.79-82, 2001
  2. Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerase produced by Penicillium pinophilum , Han, J.S.;Y.J. Son;C.S. Chang;M.N. Kim , J. Microbiol. / v.36,pp.67-73, 1998
  3. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic , Madison, L.L.;G.W. Huisman , Microbiol. Mol. Biol. Rev / v.63,pp.21-53, 1999
  4. Secretion of poly(3-hydroxybutyrate) depolymerase by Alcaligenes faecalis T1 , Zhang, J.;T. Saito;A. Ichikawa;T. Fukui , Chem. Pharm. Bull. / v.40,pp.713-717, 1992
  5. Effects of amino acid addition on molar fraction of 3-hydroxyvalerate in copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate synthesized by Alcaligenes sp , Yoon, J.S.;J.Y. Kim;Y.H. Rhee , SH-69. J. Ferment. Bioeng. / v.80,pp.350-354, 1995
  6. Bacterial polyhydroxyalkanoates , Lee, S.Y. , Biotechnol. Bioeng. / v.49,pp.1-14, 1996
  7. Microbial degradation of polyesters , Jendrossek, D. , Adv. Biochem. Eng. Biotechnol. / v.71,pp.293-325, 2001
  8. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding , Bradford, M.M. , Anal. Biochem. / v.72,pp.248-254, 1976
  9. Characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Pseudomonas alcaligenes LB19 , Kim, D.Y.;J.S. Nam;Y.H. Rhee , Biomacromolecules / v.3,pp.291-296, 2000
  10. Biodegradation of polyhydroxyalkanoic acids , Jendrossek, D.;A. Schirmer;H.G. Schlegel , Appl. Microbiol. Biotechnol. / v.46,pp.451-463, 1996
  11. Evaluation of various carbon substrates for the biosynthesis of polyhydroxyal-kanoates bearing functional groups by Pseudomonas putida , Kim, D.Y.;Y.B. Kim;Y.H. Rhee , Int. J. Biol. Macromol. / v.28,pp.23-29, 2000
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 , Laemmli, U.K. , Nature / v.227,pp.680-685, 1976
  13. Purification and properties of poly(3-hydroxybutyrate) depolymerase from the fungus Paecilomyces lilacinus D218 , Oda, Y.;H. Osaka, T.;Urakami;K. Tonomura , Curr. Microbiol. / v.34,pp.230-232, 1997
  14. Biological and enzymatic mechanisms of polyester biodegradation by fungi , Scherer, T.M. , Ph.D. Thesis. / v.,pp., 1996
  15. Substrate specificities of poly(hydroxyalkanoate)-degrading bacteria and active site studies on the extracellular poly(3-hydroxyoctanoic acid) depolymerase of Pseudomonas fluorescens GK13 , Schirmer, A.;C. Matz;D. Jendrossek , Can. J. Microbiol. / v.41,pp.170-179, 1995
  16. Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(w-hydroxyal-kanoates) , Jaeger, K.E.;A. Steinbuchel;D. Jendrossek , Appl. Environ. Microbiol. / v.61,pp.3113-3118, 1995
  17. Fungal degradation of polyhydroxyalkanoates and a semiquantitative assay for screening their degradation by terrestrial fungi , Matavulj, M.;H.P. Molitoris , FEMS Microbiol. Rev. / v.103,pp.323-332, 1992
  18. Properties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122 , Kita, K.;K. Ishimaru;M. Teraoka;H. Yanase;N. Kato , Appl. Environ. Microbiol. / v.61,pp.1727-1730, 1995
  19. Extracellular poly(3-hydroxy-butyrate) depolymerase from Penicillium funiculosum: general characteristics and active site studies , Brucato, C.L.;S.S. Wong , Arch. Biochem. Biophys. / v.290,pp.497-502, 1991

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