초록

양식어류 질병 세균에 대한 31종의 천연물을 $70\%$ ethanol로 추출하여 항균효과를 조사하였다. 그 중에서 망태버섯 ethanol 추출물이 비교적 높은 활성을 나타내었으며, 망태버섯을 극성이 낮은 dichlorornethane, ethyl acetate, n-buthanol, water의 순서로 분획하여 항균효과를 본 결과 ethyl acetate 추출물에서 모든 균주에대한 clear zone을 형성하여 항균효과가 보였다. 또한, 망태버섯로부터의 ethyl acetate 추출물을 Si-chromatography를 이용하여 fraction별 7개로 분획하여 항균효과를 조사한 결과 Fraction 2 (Hx :EtAOc, 3: 1)에서는 L. anguillarum에 대해 가장 항균효과가 뛰어났으며, Fraction 5 (DCM:MeOH, 5: 1)에서 5. iniae가 가장 높았다. 그 중에서 세 균주에 대해서 공통적으로 항균활성이 높은 것은 Fraction 5로 나타났다.

Abstract

Ethanol extract obtained from bark, roots, stem, leaves and seeds of 30 species of plants and mushroom, Dicyophora indusiata were examined antimicrobial effect on fish pathogenic bacteria, Listoneria anguillamm, Lactococcus garvieae, Streptococcus iniae by disk method. Ethanol extract of D. indusiata was found to be the most active against all fish pathogenic bacteria in test system. Ethyl acetate fraction from ethanol extract of D. indusiata showed the strongest compared with those from other solvent fractions such as dichloromethane, n-buthanol and water. The antimicrobial effect of ethyl acetate fraction was particularly evident against S. iniae.

주제어

#Antimicrobial effect   #Natural plant Dicyophora indusiata   #Fish pathogenic bacteria  

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