[논문]옻나무 껍질에서 분리한 항산화물질의 성분

Kim, Jung-Bae

옻나무 껍질에서 분리한 항산화물질의 성분
Identification of Antioxidative Component from Stem Bark of Rhus verniciflua 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.16 no.1, 2003년, pp.60 - 65

Kim, Jung-Bae (Dept. of Food and Nutrition, Sangji Youngseo College)

초록
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옻나무(Rhus verniciflua)는 한국에서 위장병, 변비, 어혈, 구충 등에 효과가 있다고 알려져 있어 민간요법과 보양식품으로 옻닭으로 식용하고 있으나 독성(allergy, 옻 오름)이 심각하여 주의를 요하는 식품이다. 옻의 독성 제거방법을 사용하여 옻나무 껍질을 물로 추출한 후 항산화 작용을 갖는 수용성 물질을 분리하여 물리, 화학적 특성과 기기 분석을 통하여 (UV, IR, EI (HR)-MS, $^1$H-NMR, $^{13}$C-NMR, elemental analyzer) 구조 분석한 결과 gallic acid (3,4,5-hydroxylbenzoic acid) 임을 확인하였다.

Abstract ▼ AI-Helper

An antioxidant compound was obtained from the water extract of the stem bark from Rhus verniciflua, which has been used in traditional folk remedies. The compound was purified by HPLC, using DEAE, CN and ODS columns. The chemical structure of the compound was identified as gallic acid (3,4,5-hydroxylbenzoic acid) by spectral data including UV, IR, EI (HR)-MS, $^1$H-NMR, $^<$TEX>13/C-NMR and elemental analyzer. This compound was found show cytotoxicity against HeLa cell ( IC$\_$50/ : 8.5$\mu\textrm{g}$/$m\ell$).

주제어

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문제 정의

In previous study¹⁷⁾, we isolated an antioxidant substance from the stem bark of Rhus verniciflua. This paper describes the structural elucidation, which an active compound was isolated and identified.

대상 데이터

After removing a compound by washing with fresh medium, the viable number was determination by MTT method. C₇H₆O₅, C₁₀H₄O₂N₁, C₅H₄O₄N₃ and C₈H₂O₁N₄. Based on the El positive mass spectrum(high resolution) in combination with ¹H and ¹³C-NMR spectral date, the molecular weight and formula of the isolated compound were found to be 170 and C₇H₆O₅, respectively.
Naturally grown Rhus verniciflua (RV) was obtained from Wonju, Kwangwon province, Korea, in June 1999. To detoxicate from preparation of RV carried out according to Kim's method^{18, 19)}.

이론/모형

The elemental composition of the HR-EI mass spectrum at m/z 170 was estimated the empirical formula asCell was incubated with a purified compound at various concentrations for 48 hr. After removing a compound by washing with fresh medium, the viable number was determination by MTT method. C₇H₆O₅, C₁₀H₄O₂N₁, C₅H₄O₄N₃ and C₈H₂O₁N₄.
The surviving cell number was determined by the MTT method²⁰⁾. In the case of HL-60RG cells, cytotoxicity was determined by the trypan blue dye exclusion method.
1ml of medium containing purified compound at appropriate concentra tions was added and the mixture was discarded and the remaining sample was removed by through washing with fresh medium. The surviving cell number was determined by the MTT method²⁰⁾. In the case of HL-60RG cells, cytotoxicity was determined by the trypan blue dye exclusion method.

성능/효과

옻의 독성 제거 방법을 사용하여 옻나무 껍 질을 물로 추출한 후 항산화 작용을 갖는 수용성 물질을 분리하여 물리, 화학적 특성과 기기 분석을 통하여 (UV,IR, El (HR)-MS, ¹H-NMR, ¹³C-NMR, elemental analy zer) 구조 분석 한 결과 gallic acid (3, 4, 5-hydroxylbenzoicacid) 임을 확인하였다.

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