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논문 상세정보

키토산올리고당이 카드뮴을 투여한 생쥐의 간독성에 미치는 효과

Effects of Chitosanoligosaccharide on the Hepatotoxicity in Cadmium-treated Mice

초록

본 연구는 카드뮴으로 유발된 생쥐 간중독에 대한 키토산올리고당의 효과를 알아보기 위하여 시도되었다. Mouse를 대상으로 카드뮴 (5.0 mg/kg; i.p.) 단독투여군(group Cd), 카드뮴과 키토산올리고당 (0.5% solution) 동시투여군 (group Cd+Chi) 그리고 키토산올리고당 1주일 전처치군 (group Chi7+Cd)으로 구분한 후 간손상 억제효과를 알아보기 위해 혈청중 효소활성도와 glutathione peroxidase (GSH-Px)의 활성도 그리고 glutathione reductase (GR)의 활성도를 비교측정하였다. 또한 간조직의 형태학적 손상을 확인하기 위해 조직학적 관찰을 실시하였다. 혈청 효소활성결과, AST, ALT 그리고 LDH의 활성도는 키토산올리고당을 처치한 Cd+Chi군과 Chi7+Cd군이 Cd군보다 감소되었다. 그러나 TP와 Alb의 활성도는 키토산올리고당을 처치한 Cd+Chi군과 Chi7+Cd군이 Cd군보다 증가되었다. GSH-Px활성은 Cd군에 비해 Cd+Chi군과 Chi7+Cd군이 현저하게 증가되었다. GR활성은 Cd군에 비해 Cd+Chi군과 Chi7+Cd군이 현저하게 감소하였다. 광학현미경적 관찰 결과, Cd군은 카드뮴 독성에 의한 간세포의 괴사가 관찰되었다. Cd+Chi군과 Chi7+Cd군은 정상군과 유사한 소견을 보였다. 이상과 같은 결과로, 키토산올리고당이 생쥐 간조직에 미치는 카드뮴의 독성을 감소시킴을 알 수 있었다.

Abstract

This research was conducted to determine the effects of chitosanoligosaccharide on liver poisoning induced by cadmium (Cd). Three groups of mice were used in this research. The first group was only injected with cadmium (5.0 mg/kg; i.p.) (group Cd) and the second one with cadmium and chitosanoligosaccharide (0.5% solution) at the same time (group Cd+Chi). The third one which had already been injeted with chitosanoligosaccharide (0.5% Solution) aweek before (group Ch7+Cd) was used. In order to investigate the inhibitory action of chitosanoligosaccharide on liver damage, enzyme activity in serum, glutathione peroxidase (GSHPx) activity and glutathione reductase (GR) activity were relatively measured. In addition, histological observations were made to determine the morphologic injury of liver tissues. As the result of enzyme activity in serum, the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in chitosanoligosaccharide-injected groups Cd+Chi and Chi7+Cd was lower than in group Cd. GSH-Px activity was sharply increased in groups Cd+Chi and Chi7+Cd compared to group Cd. GR activity was conspicuously decreased in groups Cd+Chi and Chi7+Cd compared to group Cd. As the result of light microscopic observation, liver cell necrosis caused by cadmium poisoning was obseved in liver cells. The finding of group Cd+Chi and Chi7+Cd was similar total on of normal groups. As the result of electron microscopic observation, mitochondria in group Cd showed a severe swelling phenomenon, RER fragment and ribosome dropout. However, in groups Cd+Chi and Chi7+Cd, mitochondria wiht high electron density were distributed and RER forming a typical lamellae with ribosome was observed. From these results, cadmium toxicity on rat liver tissues could be lessened by chitosanoligosaccharide.

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