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키토산올리고당이 카드뮴을 투여한 생쥐의 간독성에 미치는 효과

Effects of Chitosanoligosaccharide on the Hepatotoxicity in Cadmium-treated Mice

한국전자현미경학회지 = Korean journal of electron microscopy, v.33 no.1, 2003년, pp.59 - 78  

윤중식 (조선대학교 자연과학대학 생물과학부) ,  노영복 (조선대학교 자연과학대학 생물과학부)

초록
AI-Helper 아이콘AI-Helper

본 연구는 카드뮴으로 유발된 생쥐 간중독에 대한 키토산올리고당의 효과를 알아보기 위하여 시도되었다. Mouse를 대상으로 카드뮴 (5.0 mg/kg; i.p.) 단독투여군(group Cd), 카드뮴과 키토산올리고당 (0.5% solution) 동시투여군 (group Cd+Chi) 그리고 키토산올리고당 1주일 전처치군 (group Chi7+Cd)으로 구분한 후 간손상 억제효과를 알아보기 위해 혈청중 효소활성도와 glutathione peroxidase (GSH-Px)의 활성도 그리고 glutathione reductase (GR)의 활성도를 비교측정하였다. 또한 간조직의 형태학적 손상을 확인하기 위해 조직학적 관찰을 실시하였다. 혈청 효소활성결과, AST, ALT 그리고 LDH의 활성도는 키토산올리고당을 처치한 Cd+Chi군과 Chi7+Cd군이 Cd군보다 감소되었다. 그러나 TP와 Alb의 활성도는 키토산올리고당을 처치한 Cd+Chi군과 Chi7+Cd군이 Cd군보다 증가되었다. GSH-Px활성은 Cd군에 비해 Cd+Chi군과 Chi7+Cd군이 현저하게 증가되었다. GR활성은 Cd군에 비해 Cd+Chi군과 Chi7+Cd군이 현저하게 감소하였다. 광학현미경적 관찰 결과, Cd군은 카드뮴 독성에 의한 간세포의 괴사가 관찰되었다. Cd+Chi군과 Chi7+Cd군은 정상군과 유사한 소견을 보였다. 이상과 같은 결과로, 키토산올리고당이 생쥐 간조직에 미치는 카드뮴의 독성을 감소시킴을 알 수 있었다.

Abstract AI-Helper 아이콘AI-Helper

This research was conducted to determine the effects of chitosanoligosaccharide on liver poisoning induced by cadmium (Cd). Three groups of mice were used in this research. The first group was only injected with cadmium (5.0 mg/kg; i.p.) (group Cd) and the second one with cadmium and chitosanoligosa...

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