[논문]Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation

Song, Yong-Jin; Kwon, Seong-Chun

Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation 원문보기

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.8 no.6, 2004년, pp.313 - 317

Song, Yong-Jin (Department of Otolaryngology, Head & Neck Surgery, Kangnung Asan Hospital,Department of Physiology, College of Medicine, Kwandong University) , Kwon, Seong-Chun (Department of Physiology, College of Medicine, Kwandong University)

Abstract ▼ AI-Helper

The present study were designed to characterize the action mechanisms of acetylcholine (ACh)-induced endothelium-dependent relaxation in arteries precontracted with high $K^+$(70 mM). For this, we simultaneously measured both muscle tension and cytosolic free $Ca^{2+}$ concentration $([Ca^{2+}]_i)$, using fura-2, in endothelium-intact, rabbit carotid arterial strips. In the artery with endothelium, high $K^+$ increased both $[Ca^{2+}]_i$ and muscle tension whereas ACh $(10{\mu}M)$ significantly relaxed the muscle and increased $[Ca^{2+}]_i$. In the presence of $N^G$-nitro-L-arginine (L-NAME, 0.1 mM), ACh increased $[Ca^{2+}]_i$ without relaxing the muscle. In the artery without endothelium, high $K^+$ increased both $[Ca^{2+}]_i$ and muscle tension although ACh was ineffective. 4-DAMP (10 nM) or atropine $(0.1{\mu}M)$ abolished ACh-induced increase in $[Ca^{2+}]_i$ and relaxation. The increase of $[Ca^{2+}]_i$ and vasorelaxation by ACh was siginificantly reduced by either $3{\mu}M$ gadolinium, $10{\mu}M$ lanthanum, or by $10{\mu}M$ SKF 96365. These results suggest that in rabbit carotid artery, ACh-evoked relaxation of 70 mM $K^+$-induced contractions appears to be mediated by the release of NO. ACh-evoked vasorelaxation is mediated via the $M_3$ subtype, and activation of the $M_3$ subtype is suggested to stimulate nonselective cation channels, leading to increase of $[Ca^{2+}]_i$ in endothelial cells.

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참고문헌 (18)

Adams DJ. Ionic channels in vascular endothelial cells. Trends in Pharmacol Sci 4: 18－26, 1994
Busse R, Mulsch A. Calcium-dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin. FEBS Lett 265: 133－136, 1990

상세보기
Caldwell RA, Clemo HF, Baumgarten CM. 1998. Using gadolinium to identify stretch-activated channels: techanical considerations. Am J Physiol 275: C619－C621, 1998

상세보기
Chiba S, Tsukada M. Possible involvement of muscarinic M1 and M3 receptor subtypes mediating vasodilation in isolated, perfused canine lingual arteries. Clinical & Exp Pharmacol & Physiol 23: 839－843, 1996

상세보기
Furchgott RF, Zawadzki JV. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288: 373－376, 1980

상세보기
Kamouchi M, Mamin A, Droogmans G, Nilius B. Nonselective cation channels in endothelial cells derived from human umbilical vein. J Membrane Biol 169: 29－38, 1999

상세보기
Komori K, Suzuki H. Heterogenous distribution of muscarinic receptors in the rabbit saphenous artery. Br J Pharmacol 92: 657－664, 1987

상세보기
Koyama T, Kimura C, park SJ, Oike M, Ito Y. Functional implications of Ca2 $^+$ mobilizing properties for nitric oxide production in aortic endothelium. Life Sci 72: 511－520, 2002

상세보기
Lopez-Jaramillo P, Gonzalez MC, Palmar RMJ, Moncada S. The crucial role of physiological Ca2+ concentrations in the production of endothelial nitric oxide and the control of vascular tone. Br J Pharmacol 101: 489－493, 1990

상세보기
Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43: 109－ 142, 1991

상세보기
Miller VM, Vanhoutte PM. Endothelium-dependent vascular responsiveness: evolutionary aspects. In Endothelial Regulation of Vascular Tone (Ryan US, Rubanyi GM. eds) pp. 3－20, Marcel Dekker, Inc., New York, 1992
Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43: 109－ 142, 1991

상세보기
Nilius B, Viana F, Droogmans G. Ion channels in vascular endothelium. Ann Rev Physiol 59: 145－170, 1997

상세보기
Nilius B, Viana F, Kamouchi M, Fasolato C, Eggermont J, Droogmans G. Ca $^{2+}$ signaling in endothelial cells: role of ion channels. Korean J Physiol Pharmacol 2: 133－145, 1998

원문보기 상세보기
Sato K, Ozaki H, Karaki H. Differential effects of carbachol on cytosolic calcium levels in vascular endothelium and smooth muscle. J Pharmacol Exp Ther 255: 114－119, 1990

상세보기
Tsuchida H, Seki S, Tanaka S, Okazaki K, Namiki A. Halothane attenuates the endothelial Ca $^{2+}$ increase and vasorelaxation of vascular smooth muscle in the rat aorta. Br J Anaesth 84: 215－ 220, 2000

상세보기
Viana F, De Smedt H, Droogmans G, Nilius B. Calcium signaling through nucleotide receptor P2Y2 in cultured human vascular endothelium. Cell Calcium 24: 117－127, 1998

상세보기
Wu CC, Chen SJ, Yen MH. 1997. Loss of acetylcholine-induced relaxation by M3-receptor activation in mesenteric arteries of spontaneously hypertensive rats. J Cardiovasc Pharmacol 30: 245－252, 1997

상세보기

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Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation 원문보기

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Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation 원문보기

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