During the course of our screening for bioactive metabolites from marine sponges, EZZ, the inseparable 1:1 mixture of (8E,13Z,20Z)-strobilinin and (7E,13Z,20Z)-felixinin has been found to deliver significant cytotoxicity against some cancer cell lines. In this study, the antioxidant activity of EZZ ...
During the course of our screening for bioactive metabolites from marine sponges, EZZ, the inseparable 1:1 mixture of (8E,13Z,20Z)-strobilinin and (7E,13Z,20Z)-felixinin has been found to deliver significant cytotoxicity against some cancer cell lines. In this study, the antioxidant activity of EZZ was first time evaluated by a series of antioxidant assays. It was found that EZZ was weak in scavenging the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), but it was comparable to ascorbic acid in scavenging ABTS and superoxide radicals. In addition, EZZ could protect DNA from hydroxyl radical-induced strand cleavage. The findings of the present study suggest that EZZ possess certain antioxidant activity, which might help to prevent occurrence of cancer by alleviating the oxidative stress in cells.
During the course of our screening for bioactive metabolites from marine sponges, EZZ, the inseparable 1:1 mixture of (8E,13Z,20Z)-strobilinin and (7E,13Z,20Z)-felixinin has been found to deliver significant cytotoxicity against some cancer cell lines. In this study, the antioxidant activity of EZZ was first time evaluated by a series of antioxidant assays. It was found that EZZ was weak in scavenging the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), but it was comparable to ascorbic acid in scavenging ABTS and superoxide radicals. In addition, EZZ could protect DNA from hydroxyl radical-induced strand cleavage. The findings of the present study suggest that EZZ possess certain antioxidant activity, which might help to prevent occurrence of cancer by alleviating the oxidative stress in cells.
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가설 설정
The mechanism of EZZ based cytotoxicity and cell cycle arrest has been studied (data not shown). In this study, we evaluated the antioxidant activity of EZZ. 1, 1 -diphenyl-2-picrylhydrazyl (DPPH) and Trolox equivalent antioxidant capacity (TEAC) assay for the free radical scavenging activity, nitrobluetetrazolium (NBT) assay for the superoxide radical scavenging activity, and the inhibitory effect of hydroxyl radical-induced DNA damage were used.
참고문헌 (18)
Block, KI., Antioxidants and cancer therapy: Furthering the debate. Integr Cancer Ther. 3, 342-348 (2004)
Choi, K.T., Hong, J.K., Lee, CO., Kim, D.K., Sim, C.J., Im, KS., and lung, J.H., Cytotoxic furanosesterterpenes from a marine sponge Psammocinia W J. Nat. Prod. 67, 1186-1189 (2004a)
Choi, H.J., Kim, N.D., Jung, J.H., and Choi, YH., Regulation of Bcl-2 family and cyclooxygenases by Furanoterpenoids isolated from a marine sponge Sarcotragus sp. in human lung cancer A549 cells. J. Life Sci. 14,445-452 (2004b)
Halliwell, B. and Gutteridge, M.C, Formation of a thiobarbituricacid- reactive substance from deoxyribose in the presence of iron salts: The role of superoxide and hydroxyl radicals. FEBS Lett. 128, 347-352 (1981)
Hochstein, P and Atallah, A.S., The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer. Mutat. Res. 202, 363-375 (1988)
Keum, Y.S., Park, K.K., Lee, J.M., Chun, KS., Park, J.H., Lee, S.K, Kwon, H., and Surh, YJ., Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150, 41-48 (2000)
Miller, N.J., Castelluccio, C, Tijburg, L., and Rice-Evans, CA., The antioxidant properties of thioflavines and their gallate esters-radical scavengers or metal chelator. FEBS Letts. 392, 40-44 (1996)
Miller, N.J., Rice-Evans, CA., Davies, M.J., Gopinathan, V, and Milner, A, A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. 84,407-412 (1993)
Re, R., Pellegrini, N., Proteggente, A, Pannala, A, Yang, M., and Rice Evans, C, Antioxidant activity applying an improved ABTS radical cation decolorized assay. Free Radic. Bioi. Med. 26, 1231-1237 (1999)
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