Shin, Han-Jae
(KT&G Central Research Institute)
,
Kim, Young-Sook
(KT&G Central Research Institute)
,
Kwak, Yi-Seong
(KT&G Central Research Institute)
,
Song, Yong-Bum
(KT&G Central Research Institute)
,
Kyung, Jong-Soo
(KT&G Central Research Institute)
,
Wee, Jae-Joon
(KT&G Central Research Institute)
,
Park, Jong-Dae
(KT&G Central Research Institute)
We have recently reported that red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C. A. Meyer), showed immunomodulatory antitumor activities, mainly mediated by nitric oxide (NO) production by macrophage. In this study, we examined the effect of RGAP on antican...
We have recently reported that red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C. A. Meyer), showed immunomodulatory antitumor activities, mainly mediated by nitric oxide (NO) production by macrophage. In this study, we examined the effect of RGAP on anticancer activity using lung carcinoma 3LL, sarcoma 180 and adenocarcinoma JC tumor cells transplanted into mice as well as antimetastatic activity using B16-F10 melanoma. When RGAP (300 mg/kg) were treated to mice implanted with one of the three kinds of tumor cells, the tumor weight significantly decreased compared with control mice. Tumor inhibition ratios of RGAP (300 mg/kg) in mice transplanted with lung carcinoma 3LL, sarcoma 180 and adenocarcinoma JC cells were 26.8%, 29.3% and 31.6%, respectively. Hundred mg/kg of RGAP did not cause a significant decrease in tumor weight compared with control group. When RGAP was administered i.p. with the dose of 100 and 300 mg/kg in B16-F10 melanoma-bearing mice, lung metastasis were reduced significantly in mice. Corrected phagocytic index was also remarkably increased by RGAP. These results suggest that stimulation of phagocytic activity of macrophages may be a mechanism for in vivo anticancer and antimetastasis activities of RGAP.
We have recently reported that red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C. A. Meyer), showed immunomodulatory antitumor activities, mainly mediated by nitric oxide (NO) production by macrophage. In this study, we examined the effect of RGAP on anticancer activity using lung carcinoma 3LL, sarcoma 180 and adenocarcinoma JC tumor cells transplanted into mice as well as antimetastatic activity using B16-F10 melanoma. When RGAP (300 mg/kg) were treated to mice implanted with one of the three kinds of tumor cells, the tumor weight significantly decreased compared with control mice. Tumor inhibition ratios of RGAP (300 mg/kg) in mice transplanted with lung carcinoma 3LL, sarcoma 180 and adenocarcinoma JC cells were 26.8%, 29.3% and 31.6%, respectively. Hundred mg/kg of RGAP did not cause a significant decrease in tumor weight compared with control group. When RGAP was administered i.p. with the dose of 100 and 300 mg/kg in B16-F10 melanoma-bearing mice, lung metastasis were reduced significantly in mice. Corrected phagocytic index was also remarkably increased by RGAP. These results suggest that stimulation of phagocytic activity of macrophages may be a mechanism for in vivo anticancer and antimetastasis activities of RGAP.
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제안 방법
injection of 100 and 300 mg/kg RGAP was given to mice at the next day after subcutaneous inoculation of lung carcinoma 3LL, sarcoma 180 and adenocarcinoma JC tumor cells, respectively. After 20 to 30 days, the tumorinhibitory effect of RGAP was evaluated by measuring tumor weight both of control group and RGAP-treated mice group. As shown in Table 1, 2, 3 when RGAP (300 mg/kg) was treated to mice implanted with one of the three kinds of tumor cells, the tumor weight was significantly decreased as compared with that of control mice (p<0.
Inhibition effect of RGAP on lung metastasis in B16-F10 melanoma tumor-bearing mice. Five C57BL/6 mice per group were inoculated i.v. with B16-F10 melanoma (2xl05) and administered i.p. with RGAP for seven consecutive days after tumor inoculation. On day 15 after tumor inoculation, mice were killed and lung metastases were observed.
, 2002). In this study, for complete confirmation as to antitumor activity of RGAP we examined the effect of RGAP on the growth of sarcoma-180, 3LL lung carcinoma and JC mammary adenocarcinoma transplanted subcutaneously into mice and on the lung metastasis produced by B16-F10 melanoma.
2 ml of tumor cells (*) 2x10 in PBS. The mice were randomly assigned to control and RGAP treated groups (100 or 300 mg/kg) at one day after the implantation. On day 30, the mice were sacrificed and tumor weights were measured.
2 ml/mouse. The mice were randomly divided into control and RGAP treated groups (100 or 300 mg/kg) at one day after the implantation. On day 22, the mice were sacrificed and tumor weights were measured.
, 1996). We therefore, tried to investigate whether or not RGAP could inhibit lung metastasis produced by the i.v. inoculation of B16-F10 melanoma cells. As shown in Fig.
대상 데이터
Specific pathogen-free female C57BL/6, BA LB/c, BDF1 and ICR mice were purchased from Daehan Laboratory Animal Research Center Co. (Eumsong, Korea) and acclimated for at least 1 week. All animals were maintained on gamma-irradiated Jeil Lab Chow (Daejon, Korea) and ultraviolet-irradiated tap water ad libitum.
데이터처리
Data obtained from the pharmacological experiments were expressed as mean± S.D. Differences between the control and test groups in this experiment were measured for statistical significance by Student's t-test or analysis of variance (ANOVA).
이론/모형
Phagocytic function was determined by the method of Biozzi et al. (1954). One day after 7 consecutive RGAP administration once daily, a suspension of carbon particle (Rotring Drowing Ink, Germany) in 1% gelatin solution (1:5) was injected to ICR mice via tail vain (0.
성능/효과
In conclusion, this study demonstrated that administration of RGAP inhibited growth of several tumor cells in vivo and prevented lung metastasis of B16-F10 melanoma in mice and that the antitumor action of RGAP was associated with the activation of non-specific phagocytosis as well as augmented TNF-α and NO production of macrophages.
The concentrates was dialyzed against water to completely remove small molecules less than 10 KDa to obtain non-dialyzable fraction consisting of acidic polysaccharide (yield : about 5%). RGAP was confirmed to be the acidic polysaccharide fraction composed of about 56.9% uronic acid, 28.3% neutrai sugar and less than 1% protein. The analysis of component sugars in RGAP by GC revealed that the polysaccharides in RGAP contained about 51.
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