[논문]In vitro Inhibitory Activities of Essential Oils from Oenanthe javanica DC against Candida and Streptococcus species

Shin, Seung-Won

In vitro Inhibitory Activities of Essential Oils from Oenanthe javanica DC against Candida and Streptococcus species 원문보기

Natural product sciences, v.10 no.6, 2004년, pp.325 - 329

Shin, Seung-Won (Duksung Women's University, College of Pharmacy)

Abstract ▼ AI-Helper

The composition of essential oil from O. javanica was analyzed by gas chromatography-mass spectrometry. Using the broth dilution method and disk diffusion test, anti-microbial activities of the oil fraction and its main components were evaluated against various antibiotic-susceptible and resistant strains of pathogenic microorganisms. As a result of GC-MS analysis, 57 compounds, including ${\alpha}-terpinolene$ (28.1%), dl-limonene (16.0%), ${\gamma}-terpinene$ (10.3%), ${\beta}-pinene$ (9.7%) and ${\alpha}-pinene$ (6.0%) were identified in the essential oil fraction. The essential oil fraction of O. javanica and its main components exhibited significant inhibitory activities, particularly against Candida albicans (antibiotic-susceptible strains) and Streptococcus pneumoniae (antibiotic- susceptible and resistant strains). The main components of the O. javanica oil fraction displayed different patterns of activity against the three tested Candida species as exemplified by the differential minimum inhibiting concentration (MIC) values. The disk diffusion test showed that the activities were dose dependent.

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제안 방법

Essential oil fractions were analyzed by GC-FID and GC-MSD (Agilent 5973 network mass selective detector, 280℃) and the data obtained was compared with standards. Also, a Wiley 275 database search was performed on the data obtained from the analysis of the essential oils on a Hewlett-Packard 6890 GC system and a 5973 MSD apparatus equipped with a fused silica capillary column (HP-5 MS 5% phenyl methyl siloxane, 30m × 250㎛ × 0.25㎛, respectively. The injector was adjusted to 250℃ and the oven temperature was set as follows: initial temperature: 50℃(isothermal, 5 min), increased to 2℃/min to 180℃, 5 min at 180℃, and 180- 220℃ (20℃/min).
, 1981; May et al, 1995). In this study, we analyzed essential oils from O. javanica and screened them for anti-microbial activity against selected pathogenic gram-positive and gramnegative bacteria, and fungi. In particular, growth-inhibition of Streptococcus pneumoniae and three common pathogenic Candida species was evaluated using the broth dilution method and disk diffusion test.
javanica, resulting in more than a 30 mm growth inhibitory zone on agar culture plates in the disk diffusion test (Table 2). On the basis of these results, experiments were conducted using a ten-fold serially diluted oil suspension with culture medium containing Tween-80, with a view to determining the activity of this oil againstvarious strains of C. albicans and S. pneumoniae. S.

대상 데이터

The tested strains tested were obtained from the Culture Collection of Antibiotic Resistant Microbes (CCARM) and the Korean Culture Center of Micro-oganisms (KCCM).

이론/모형

MIC values of the oils were determined by the broth microdilution method. A range of two-fold dilutions (160- 0.
Among the samples tested, limonene and a-pinene showed relatively stronger activity, with MIC vahies from 4 mg/ml to 16 mg/ml, compared to the other compounds tested. These results were confirmed by employing the disk diffusion test on Sabouraud agar plates using ketoconazole as control. The width of the inhibitory zone of fungal growth by the samples are listed in Table 5.

성능/효과

In conclusion, the essential oil of O. javanica may be used as an effective agent against Candida and S. pneumoniae infections, and particularly against antibioticresistant strains of these organisms. Although its activity is much weaker than those of synthetic antibiotics, it is an attractive and effective agent against these pathogenic microorganisms, particularly in clinical situations where the unpleasant side-effects and toxicity of synthetic antibiotics limit their therapeutic use, such as in immunosuppressed patients (Eggimann et al, 2003; Shin, 2004).
As shown in Table 4, the 0. javanica oil fraction and its main components, β-pinene, α-pinene, α-terpinolene, 𝛶-terpinene, and limonene, displayed distinct patterns of activity against the three tested Candida species as shown by their differential MIC values (ranging from 4 mg/ml to 64 mg/ml). Among the samples tested, limonene and a-pinene showed relatively stronger activity, with MIC vahies from 4 mg/ml to 16 mg/ml, compared to the other compounds tested.

후속연구

Although its activity is much weaker than those of synthetic antibiotics, it is an attractive and effective agent against these pathogenic microorganisms, particularly in clinical situations where the unpleasant side-effects and toxicity of synthetic antibiotics limit their therapeutic use, such as in immunosuppressed patients (Eggimann et al, 2003; Shin, 2004). Further studies will be required to analyze the effects of the essential oil of O. javanica on drug-resistant Candida species.

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원문보기 상세보기
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