• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

이유자돈 전신소모성증후군 이환 자돈에서의 바이러스성 원인체 검색 및 porcine circovirus 2 분리동정

Detection of viral pathogens and isolation of porcine circovirus 2 from postweaning multisystemic wasting syndrome-affected piglets


To detect viral agents and isolate porcine circovirus 2 (PCV2), 60 samples of lung and lymph node were collected from 5 to 12 week-old pigs that had showed clinical signs of postweaning multisystemic wasting syndrome (PMWS). Polymerase chain reactions (PCRs) were conducted to identify the viral pathogens including PCV1, PCV2, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) that have been considered to be the causal agents of PMWS. Among 60 samples, PCV 2 was detected from 57 samples but no PCV 1 was detected. PRRSV and/or PPV were also detected from 27 (47.4%) samples and 1 (1.8%) sample of these 57 PCV 2-positive samples, respectively. Tissue homogenates were inoculated onto PCV-free PK-15 cell monolayers. Seven isolates were confirmed as PCV 2 by multiplex PCR, indirect immunofluorescence assay, and transmissible electron microscopy. These date suggest that PRRSV is a major cofactors causing PMWS in pigs that were infected with PCV2 in Korea.

참고문헌 (42)

  1. Allan, G. M. and Ellis, J. A. Porcine circoviruses: a review. J. Vet. Diagn. Invest. 2000, 12, 3-14 
  2. Ellis, J., Hassard, L., Clark, W., Harding, J., Allan, G., Willson, P., Strokappe, J., Martin, K., McNeilly, F., Meehan, B., Todd, D. and Haines, D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can. Vet. J. 1998, 39, 41-51 
  3. Krakowka, S., Ellis, J. A., Meehan, B., Kennedy, S., McNeilly, F. and Allan, G. Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet. Pathol. 2000, 37, 254-263 
  4. LeCann, P., Albina, E., Madec, F., Cariolet, R. and Jestin, A. Piglet wasting disease. Vet. Rec. 1997, 141, 660 
  5. Lukert, P., de Boer, G. F., Dale, J. L.. The Circoviridae. In: Murphy, F. A., Fauquet, C. M. and Bishop, D. H. L. Virus Toxonomy. 6th Rep. Int. Committee Taxon. viruses. pp. 166-168, Vienna: Springer-Verlag, 1995 
  6. Mankertz, A., Domingo, M., Folch, J. M., LeCann, P., Jestin, A., Segales, J., Chmielewicz, B., Plana-Duran, J. and Soike, D. Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Res. 2000, 66, 65-77 
  7. Sambrook, J. F., Russell, D. W. and Irwin, N. Molecular Cloning: A laboratory Manual. 3rd ed, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2000 
  8. Sorden, S. D., Harms, P. A., Nawagitgul, P., Cavanaugh, D. and Paul, P. S. Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embeded tissue. J. Vet. Diagn. Invest. 1999, 11. 528-530 
  9. Tischer, I., Bode, L., Peters, D., Pociuli, S. and Germann B. Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Arch. Virol. 1995, 140, 737-743 
  10. Tischer, I., Rasch, R. and Tochtermann, G. Characterization of papovavirus and picornavirus-like particles in permanent piglet kidney cell lines. Zentralbl. Bakteriol. Orig. A. 1974, 226, 153-167 
  11. McNeilly, F., McNair, I., Mackie, D. P., Meehan, B. M., Kennedy, S., Moffett, D., Ellis, J., Krakowka, S. and Allan, G. M. Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2. Arch. Virol. 2001, 146, 909-922 
  12. Nayar, G. P., Hamel, A. and Lin, L. Detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Can. Vet. J. 1997, 38, 385-386 
  13. Balasch, M., Segales, J., Rosell, C., Domingo, M., Mankertz, A., Urniza, A. and Plana-Duran, J. Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome. J. Comp. Pathol. 1999, 121, 139-148 
  14. Choi, C. and Chae, C. In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J. Comp. Pathol. 1999, 21, 65-270 
  15. Hamel, A. L., Lin, L. L. and Nayar, G. P. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J. Virol. 1998, 72, 5262-5267 
  16. Tischer, I., Bode, L., Apodaca, J., Timm, H., Peters, D., Rasch, R., Pociuli, S. and Gerike, E. Presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle. Arch. Virol. 1995, 140, 1427-1439 
  17. Harding, J. Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. Proc. Am. Assoc. Swine Pract. 1997, 28, 503 
  18. Hines, R. K. and Lukert, D. Porcine circovirus: A serological survey of swine in the United States. Swine Health Prod. 1995, 3, 71-73 
  19. Lukert, P. D. Disease of swine. pp. 119-131, 8th ed. Iowa State University Press, Ames, Iowa, 1999 
  20. Allan, G. M., Kennedy, S., McNeilly, F., Foster, J. C., Ellis, J. A., Krakowka, S. J., Meehan, B. M. and Adair, B. M. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J. Comp. Pathol. 1999, 121, 1-11 
  21. Morozov, I., Sirinarumitr, T., Sorden, S. D., Halbur, P. G., Morgan, M. K., Yoon, K. J. and Paul, P. S. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J. Clin. Microbiol. 1998, 36, 2535-2541 
  22. Ellis, J. A., Bratanich, A., Clark, E. G., Allan, G., Meehan, B., Haines, D. M., Harding, J., West, K. H., Krakowka, S., Konoby, C., Hassard, L., Martin, K. and McNeilly, F. Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J. Vet. Diagn. Invest. 2000, 12, 21-27 
  23. Choi, C., Chae, C. and Clark, E. G. Porcine postweaning multi-systemic wasting syndrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain reaction. J. Vet. Diagn. Invest. 2000, 12, 151-153 
  24. Dulac, G. C. and Afshar, A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 1989, 53, 431-433 
  25. Park, C. K., Joo, H. D., Shin, J. H., Kim, J. H., Sohn, H. J., Kim, K. S. and Kang, Y. B. Annual research report of National Veterinary Research and Qurantine Service (NVRQS) in 1999. NVRQS, Korea. 2000 
  26. Lyoo, Y. S., Park, C. K. and Lee, C. H. RT-PCR and nested PCR amplification of the PRRSV genes from boar semen for the rapid and sensitive differential diagnosis. Korean J. Vet. Res. 1998, 38, 77-83 
  27. Stevenson, G. W., Kiupel, M., Mittal, S. K. and Kanitz, C. L. Ultrastructure of porcine circovirus in persistently infected PK-15 cells. Vet. Pathol. 1999, 36, 368-378 
  28. Allan, G. M., McNeilly, F., Kennedy, S., Daft, B., Clark, E. G., Ellis, J. A., Haines, D. M., Meehan, B. M. and Adair B. M. Isolation of porcine circoviruslike viruses from pigs with a wasting disease in the USA and Europe. J. Vet. Diagn. Invest. 1998, 10, 3-10 
  29. Walker, I. W., Konoby, C. A., Jewhurst, V. A., McNair, I., McNeilly, F., Meehan, B. M., Cottrell, T. S., Ellis, J. A. and Allan, G. M. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J. Vet. Diagn. Invest. 2000, 12, 400-405 
  30. Madec, F., Eveno, E., Morvan, P., Hamon, L., Blanchard, P., Cariolet, R., Amenna, N., Morvan, H., Truong, C., Mahe, D., Albina, E. and Jestin, A. Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: clinical observations from follow-up studies on affected farms. Livest. Prod. Sci. 2000, 63, 223-233 
  31. Onuki, A., Abe, K., Togashi, K., Kawashima, K., Taneichi, A. and Tsunemitsu, H. Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. J. Vet. Med. Sci. 1999, 61, 1119-1123 
  32. Tischer, I., Mields, W., Wolff, D., Vagt, M. and Griem, W. Studies on epidemiology and pathogenicity of porcine circovirus. Arch. Virol. 1986, 91, 271-276 
  33. Tischer, I., Peters, D., Rasch, R. and Pociuli, S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch. Virol. 1987, 96, 39-57 
  34. Clark, E. G. Post-weaning multisystemic wasting syndrome. Proc. Am. Assoc. Swine Pract. 1997, 28, 499-501 
  35. Meehan, B. M., Creelan, J. L., McNulty, M. S. and Todd, D. Sequence of porcine circovirus DNA: affinities with plant circoviruses. J. Gen. Virol. 1997, 78, 221-227 
  36. Allan, G. M., McNeilly, F., Cassidy, J. P., Reilly, G. A., Adair, B., Ellis, W. A. and McNulty, M. S. Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet. Microbiol. 1995, 44, 49-64 
  37. Larochelle, R., Antaya, M., Morin, M. and Magar, R. Typing of porcine circovirus in clinical specimens by multiplex PCR. J. Virol. Methods. 1999, 80, 69-75 
  38. Lyoo, K. S., Park, Y. H. and Park, B. K. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea. J. Vet. Sci. 2001, 2, 201-207 
  39. Hines, R. K. and Lukert, D. Porcine circovirus as a cause of congenital tremors in newborn pigs. Proc. Am. Assoc. Swine Pract. 1994, 25, 344-345 
  40. Ouardani, M., Wilson, L., Jette, R., Montpetit, C. and Dea, S. Multiplex PCR for detection and typing of porcine circoviruses. J. Clin. Microbiol. 1999, 37, 3917-3924 
  41. Ellis, J., Krakowka, S., Lairmore, M., Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S. and McNeilly, F. Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J. Vet. Diagn. Invest. 1999, 11, 3-14 
  42. Lyoo, Y. S., Kim, J. H. and Park, C. K. Identification of porcine circoviruses with genetic variation from lymph nodes collected in pigs with PMWS. Korean J. Vet. Res. 1999, 39, 353-358 

이 논문을 인용한 문헌 (2)

  1. Kim, Eun-Gyeong ; Hwang, Bo-Won ; Lee, Jong-Min ; Son, Byeong-Guk ; Park, Ho-Jung ; Kim, Tho-Kyoung 2009. "Rapid detection and quantification of porcine circovirus type 2 (PCV 2) DNA in Real-time PCR" 韓國家畜衛生學會誌 = Korean journal of veterinary service, 32(4): 299~306 
  2. Chu, Keum-Suk 2011. "Application of a nested-polymerase chain reaction assay to differentiate the genotypes of porcine circovirus 2" 韓國家畜衛生學會誌 = Korean journal of veterinary service, 34(1): 13~18 


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일