Abstract

The expression of nestin and vimentin in the spinal nerve roots of rats with experimental autoimmune encephalomyelitis (EAE) was studied to ascertain whether Schwann cells in the peripheral nerves respond to acute central nervous system autoimmune injury. Immunohistochemistry demonstrated that nestin was constitutively expressed in the dorsal roots of spinal nerves in control rats; its expression was enhanced in the spinal nerve roots of rats with EAE. Vimentin expression was weak in control rat spinal nerve roots, and it was increased in the dorsal roots of rats with EAE. It is postulated that normal animals have multipotent progenitor cells that constitutively express nestin and vimentin in the spinal nerve roots. In response to an injury of the central nervous system, these multipotent Schwann cells are activated in the spinal nerve roots through the expression of the intermediate filament proteins vimentin and nestin.

주제어

#autoimmune encephalomyelitis   #Schwann cells   #nestin   #vimentin  

참고문헌 (14)

1. Pender, M. P. Recovery from acute experimental allergic encephalomyelitis in the Lewis rat. Early restoration of nerve conduction and repair by Schwann cells and oligodendrocytes. Brain 1989, 112, 393-416 
2. Wei, L. C., Shi, M., Chen, L. W., Cao, R., Zhang, P. and Chan, Y. S. Nestin-containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of postnatal developing and adult mice. Brain Res. Dev. Brain Res. 2002, 139, 9-17 
3. Eliasson, C., Sahlgren, C., Berthold, C. H., Stakeberg, J., Celis, J. E., Betsholtz, C., Eriksson, J. E. and Pekny, M. Intermediate filament protein partnership in astrocytes. J. Biol. Chem. 1999, 274, 23996-24006 
4. Moon, C., Kim, S., Wie, M., Kim, H., Cheong, J., Park, J., Jee, Y., Tanuma, N., Matsumoto, Y. and Shin, T. Increased expression of p53 and Bax in the spinal cords of rats with experimental autoimmune encephalomyelitis. Neurosci. Lett. 2000, 289, 41-44 
5. Calvo, J. L., Carbonell, A. L. and Boya, J. Coexpression of glial fibrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats. Brain Res. 1991, 566, 333-336 
6. Moon, C., Lee, Y. and Shin, T. Immunohistochemical localization of Bcl-2 in the spinal cords of rats with experimental autoimmune encephalomyelitis. J. Vet. Sci. 2002, 3, 279-283 
7. Cammer, W., Tansey, F. A. and Brosnan, C.F. Gliosis in the spinal cords of rats with experimental allergic encephalomyelitis: immunostaining of carbonic anhydrase and vimentin in reactive astrocytes. Glia 1989, 2, 223-230 
8. Park, M. S., Lee, J. M., Sim, K. B. and Shin, T. Transplantation of olfactory bulb promote locomotor recovery in dogs with spinal cord hemisection. Korean J. Lab. Anim. Sci. 2003, 19, 271-275 
9. Shin, T., Ahn, M., Jung, K., Heo, S., Kim, D., Jee, Y., Lim, Y. K. and Yeo, E. J. Activation of mitogenactivated protein kinases in experimental autoimmune encephalomyelitis. J. Neuroimmunol. 2003, 140, 118-125 
10. Shin, T., Lee, Y. and Sim, K. B. Embryonic intermediate filaments, nestin and vimentin, expression in the spinal cords of rats with experimental autoimmune encephalomyelitis. J. Vet. Sci. 2003, 4, 9-13 
11. Shin, T., Kojima, T., Tanuma, N., Ishihara, Y. and Matsumoto, Y. The subarachnoid space as a site for precursor T cell proliferation and effector T cell selection in experimental autoimmune encephalomyelitis. J. Neuroimmunol. 1995, 56, 171-178 
12. Dijkstra, C. D., Dopp, E. A., Joling, P. and Kraal, G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 1985, 54, 589-599 
13. Raine, C. S. The Dale E. McFarlin Memorial Lecture: the immunology of the multiple sclerosis lesion. Ann. Neurol. 1994, 36, S61-72 
14. Dal Canto, M. C. and Lipton, H. L. Schwann cell remyelination and recurrent demyelination in the central nervous system of mice infected with attenuated Theiler's virus. Am. J. Pathol. 1980, 98, 101-122 

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