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In many organisms, trehalose has been Down as an energy source and a protectant against various environmental stresses such as desiccation, freezing, heat and osmotic pressure. Previously, we have isolated and characterized the genes encoding trehalose-6-phosphate synthase (ZrTPS1) and trehalose-6-phosphate phosphatase (ZrTPS2) from one of the most osmotolerant yeasts, Zygosaccharomyces rouxii. We have also generated transgenic plants by co-introduction of ZrTPS2 and ZrTPS2 into potato plant (ZrTPS2-2A-ZrTPS1 plant) in an attempt to metabolically engineer trehalose in the transgenic plant using the foot-and-mouth disease virus(FMDV) 2A system and to generate drought resistant crop plants. In this research, we assayed previously generated the ZrTPS2-2A-ZrTPS1 plant biofunctionally by drought treatment, and measured the amount of trehalose in the ZrTPS2-2A-ZrTPS1 transgenic plants. The ZrTPS2-2A-ZrTPS1 transgenic plant showed strong drought resistance in spite of little or no accumulation of transgenic in he transgenic plant compared with control plant.

참고문헌 (16)

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  2. Thevelein, J. M (1984) Regulation of trehalose mobili-zation in fungi, Microbiot. Rev. 48, 42-59 
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  5. Heutherio, E. C, Araujo, P. S. and Panek, A. D. (1993)Role of the trehalose carrier in dehydration resistance ofSaccharormyces cerevisiae, Biochim. Biophys. Acta 1156, 263-266 
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  9. De virgilio, C, Piper, P., Boller, T. and Wiemken, A.(1991) Acquisition of thermotolerance in Sacchccromyces cerevisiae without heat shock protein hsp104 and in the absence of protein synthesis, FEBS Lett. 288, 86-90 
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  12. Kwon, H. B., Yeo, E. T., Han, S. E., Bae, S. C, Kim, D.Y. and Byun, M. O. (2003) Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPSl) and trehalose-6-phosphate phosphatase (TPS2) from Zyg-osaccharomyces rouxii, FEMS Yeast Research 1557, 1-8 
  13. Yeo, E. T., Kwon, H. B., Lee, J. T., Ryu, J. C and Byun, M. O. (2000) Genetic engineering of drought resistant potato plants by introduction of the treha-lose-6-phosphate synthase (TPSl) gene from Saccharom-yces cerevisiae, Molecules and Cells 10, 263-268 
  14. Romero, C, Belles, J. M, Vaya, J. L, Serrano, M and trehalose-6-phosphate synthase gene in transgenic tobacco plats: pleiotropic phenotype indude drought resis-tance, Planta 201, 293-297 
  15. Pilon-Smits, E. A. H., Terry, N., Sears, T., Kim, H.,Zayed, A., Hwang, S., van Dun, K., Voogd, E, Verw-oerd, T. C., Krutwagen, R. W. (1998) Trehalose produ-ong transgenic tobacco plants showed improved growth performance under drought stress, J. Plant Physiol. 152,525-532 
  16. Goddijn, O. J. M, Verwoerd, T. C., Voogd, E., Krutwagen, R. T. H., Graff, P. T. H,, Poels, J, van Dun, K.,Ponstein, A. S., Damm, B. and Pen, J. (1997) Inhibition of trehalose activity enhances trehalose accumulation in transgenic plants, Ptant Physiol. 113, 181-190 

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