The Separation of two amino acids, phenylalanine and tryptophan, was carried out using laboratory simulated moving bed (SMB) chromatography. The SMB process consisted of four zones, with each zone having 2 columns. The triangle theory was used to obtain the operating conditions for the SMB. The mass...
The Separation of two amino acids, phenylalanine and tryptophan, was carried out using laboratory simulated moving bed (SMB) chromatography. The SMB process consisted of four zones, with each zone having 2 columns. The triangle theory was used to obtain the operating conditions for the SMB. The mass transfer coefficients of the two amino acids were obtained from the best-fit values by comparing simulated and experimental pulse data. The competitive adsorption isotherms of the two amino acids were obtained by single and binary frontal analyses, taking into consideration the competition between the two components. A competitive Langmuir isotherm, obtained from single-component frontal chromatography, was used in the first run, and the isotherm from binary frontal chromatography in the second, with the flow rate of zone 1 modified to improve the purity. Compared to the first and second runs, the competitive Langmuir isotherm from the binary frontal chromatography Showed good agreement with the experimental results. Also, adjusting the flow rate in zone 1 increased the purity of the products. The purities of the phenylalanine in the raffinate and the tryptophan in the extract were 99.84 and $99.99\%$, respectively.
The Separation of two amino acids, phenylalanine and tryptophan, was carried out using laboratory simulated moving bed (SMB) chromatography. The SMB process consisted of four zones, with each zone having 2 columns. The triangle theory was used to obtain the operating conditions for the SMB. The mass transfer coefficients of the two amino acids were obtained from the best-fit values by comparing simulated and experimental pulse data. The competitive adsorption isotherms of the two amino acids were obtained by single and binary frontal analyses, taking into consideration the competition between the two components. A competitive Langmuir isotherm, obtained from single-component frontal chromatography, was used in the first run, and the isotherm from binary frontal chromatography in the second, with the flow rate of zone 1 modified to improve the purity. Compared to the first and second runs, the competitive Langmuir isotherm from the binary frontal chromatography Showed good agreement with the experimental results. Also, adjusting the flow rate in zone 1 increased the purity of the products. The purities of the phenylalanine in the raffinate and the tryptophan in the extract were 99.84 and $99.99\%$, respectively.
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문제 정의
The objective of this study was to obtain the optimal operating conditions for phenylalanine and tryptophan separation in an SMB run. Competitive Langmuir isotherms, from single - component frontal and binary frontal analyses, were compared in term of the SMB separation, and the results of the experimental and simulation works compared.
제안 방법
In this work, a binary frontal analysis was performed over a wide concentration range, with mixtures of phenylalanine and tryptophan. The results were as follows;
The intraparticle and total porosities were obtained from Blue Dextran and NaCl pulse tests, respectively, with the interparticle porosity calculated from the total porosity. The single-component isotherms of the two amino acids were determined by multiple frontal analyses of each component, The isotherms were obtained by stepwise changing of the amino acids concentrations.
The competitive Langmuir isotherms from a multi-component frontal analysis gave a more adequate isotherm, as proved experimentally. To improve the purity and yield, the zone flow rates were modified. The experimental results showed better separation of the phenylalanine and tryptophan when the competition between these components was considered, with subsequent changes in the zone I flow rate.
대상 데이터
A laboratory four-zone SMB was used for the experiments, which was composed of 8 columns (21.7 × 2.5 cm ID), with each zone having two columns. Two FPLC pumps controlled the feed and desorbent streams, and two FMI (Fluid Metering, NY, USA) pumps controlled the extract and recycle streams.
Two FPLC pumps controlled the feed and desorbent streams, and two FMI (Fluid Metering, NY, USA) pumps controlled the extract and recycle streams. Eight rotary valves (VICI, Switzerland), with 1 inlet and 8 outlet ports, were used. Each column had one rotary valve connected to the four streams.
The HPLC grade acetonitrile was purchased from Fisher Scientific (NJ, USA), and was used with deionized water as the mobile phase in the HPLC assay. The PVP resin (poly-4-vinylpyridine cross-linked, Reillex HP polymer) was purchased from Reilly Industries (Indianapolis, IN, USA). The resin was washed with 1 M NaOH, followed by 1 M HCI, and then by 50% NaOH, for 3-4 bed volumes.
데이터처리
The mass transfer coefficient was obtained by comparing the simulated with the experimental pulses of the amino acids. The axial dispersion and diffusivity were estimated from the breakthrough curves of the frontal chromatography, using the same method as for the mass transfer coefficient.
이론/모형
Single component Langmuir isotherms of phenylalanine (a) and tryptophan (b). The circles were calculated from multi ple frontal analysis and solid line fitted for the Langmuir iso therm model.
The system parameters for simulation of the SMB operation are listed in Table 1. The simulation was performed using Aspen chromatography, with the operating conditions obtained using the Triangle theory. The mass transfer coefficient was obtained by comparing the simulated with the experimental pulses of the amino acids.
성능/효과
to obtain the isotherms of the two amino acids, as shown in Fig. 3. The experimental and simulated results of the multiple frontal analyses for phenylalanine and tryptophan showed good agreement. The Langmuir isotherm was used to fit the function of at a given c, as presented in Fig.
80%, respectively. The experimental results showed purities for the phenylalanine in the raffinate and the tryptophan in the extract of 94.58 and 99.99%, respectively. The yields of the phenylalanine in the raffinate and the tryptophan in the extract were 99.
To improve the purity and yield, the zone flow rates were modified. The experimental results showed better separation of the phenylalanine and tryptophan when the competition between these components was considered, with subsequent changes in the zone I flow rate. Compared to the first and second runs, the competitive Langmuir isotherm from the binary frontal chromatography showed good agreement with the experimental results.
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