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In order to produce a harmful protein more efficiently, this expression cassette, dubbed pCol-MICT, is directed by the colicin promoter, and was constructed by the insertion of a $rrnBT_1T_2$ fragment of pEXP7, and a MxelnteinCBD fragment of pTXB3, into pSH375. To test whether harmful proteins, including proteolytic enzymes, could be effectively produced by this cassette, the carboxypeptidase (CPase) Taq gene was inserted into the pCol-MICT cassette to yield pCol-CPase Taq-MICT. E coli W3l 10 tells harboring pCol-CPase Taq-MICT produced a large quantity of this enzyme, as much as 47.2 mg of purified from per liter of culture, when cultured in the presence of mitomycin C ($0.4{\mu}g/mL$). This indicates that the colicin promoter-controlled E, coli expression cassette was able to produce almost 8 times of protein than the conventional tar promoter-based system, and that this cassette may be useful in the Synthesis of other harmful proteins.

참고문헌 (14)

  1. Hong, J.-K., M.-S. Park, F M. Raushel, and Y-H. Khang (2003) Expression and characterization of Escherichia coli prolidase with organophosphorus compounds. Biotechnol. Bioprocess Eng. 8: 126-129 
  2. Lee, S.-H., H. Taguchi, E. Yoshimura, E. Minagawa, S. Kaminogawa, T. Ohta, and H. Matsuzawa (1994) Carboxypeptidase Taq, a thermostable zinc enzyme, from Therrnus aquaticus YT-1: Molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli. Biosci. Biotech. Biochem. 58: 1490-1495 
  3. Ebina, Y., Y. Takahara, K. Shirabe, M. Yamada, T. Nakazawa, and A. Nakazawa (1983) Plasmid-encoded regulation of colicin El gene expression. J. Bacteriol. 156: 487-492 
  4. Parker, R. C. (1986) Mitomycin C-induced bidirectional transcription from the colicin E1 promoter region in plasmid ColE1. Biochirn. Biophys. Acta 868: 39-44 
  5. Petit, M. A, W. Bedale, J. Osipiuk, C. Lu, M. Rajagopalan, P. McInerney, M. F Goodman, H. Echols (1994) Sequential folding of UmuC by the Hsp70 and Hsp60 chaperone complexes of Escherichia coli. J. Bioi. Chem. 269: 23824-23829 
  6. Sanger, F., S. Nicklen, and A. R. Coulson (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467 
  7. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
  8. Masaki, H., A Akutsu, T. Uozumi, and T. Ohta (1991) Identification of a unique specificity determinant of the colicin E3 immunity protein. Gene 107: 133-138 
  9. Rosen, H. (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. Biophys. 67: 10-15 
  10. Masaki, H. and T. Ohta (1985) Colicin E3 and its immunity genes. J. Mol. Biol. 182: 217-227 
  11. Waleh, N. S., I. Sohel, J. B. Lazar, D. V. Hudson, P. Sze, P. A. Underhill, and P. H. Johnson (1992) Expression of synthetic genes encoding fused proteins under tight control of modified regulatory regions of the colicin operon. Gene 117: 7-14 
  12. Cavard, D., S. P. Howard, R. Lloubes, and C. Lazdunski (1989) High-level expression of the colicin A lysis protein. Mol. Gen. Genet. 217: 511-519 
  13. Waleh, N. S. and P. H. Johnson (1985) Structural and functional organization of the colicin E1 operon. Proc. Natl. Acad. Sci. USA 82: 8389-8393 
  14. Van den Elzen, P. J., M. J. Maat, H. H. B., Walters, E. Veltkamp, and H. J. Nijkamp (1982) The nucleotide sequence of the bacteriocin promoters of plasmids CIo DF13 and Col E1: Role of lexA repressor and cAmp in the regulation of promoter activity. Nucleic Acids Res. 10: 1913-1928 

이 논문을 인용한 문헌 (3)

  1. 2005. "" Biotechnology and bioprocess engineering, 10(3): 225~229 
  2. 2008. "" Biotechnology and bioprocess engineering, 13(4): 470~475 
  3. Xu, Ming-Lu ; Wang, Lan ; Xu, Gui-Fang ; Wang, Myeong-Hyeon 2011. "Antidiabetes and Angiotensin Converting Enzyme Inhibitory Activity of Sonchus asper (L) Hill Extract" 생약학회지, 42(1): 61~67 


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