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논문 상세정보

동충하초(冬蟲夏草)의 단백다당류 생성 특성

Properties in Formation of Protein-Binding Polysaccharide in Cordyceps militaris

초록

국내에서 재배되고 있는 번데기동충하초의 배양조건과 영양원이 균사체의 생육과 단백다당류 생성에 미치는 영향을 조사한 결과는 다음과 같다. 탄소원에 따른 단백다당류의 생성량은 균체와 배양액에서 glucose, sucrose, 전분의 순으로 30mg/100$m\ell$ 이상의 생성량을 보였다. 또한 탄소원에 따른 단백다당류를 구성하는 단당류의 조성은 크게 달라졌다. 탄소원과 질소원에 따른 단백다당류의 생성은 glucose와 peptone에서 가장 높았으며 탄소원과 질소원의 비율(C/N비)은 glucose 3%에 peptone 0.5%의 함량이 균체의 증식이나 단백다당류 생성에 가장 좋았다. 단백다당류의 생성량은 균사체 내에서는 진탕 배양에 의해 현저하게 증가하였으며 배지의 산도는 pH 4.5에서 최적을 보였으며 최적배양온도는 $25^{\circ}C$이었다. DPPH 법으로 측정한 단백다당류의 항산화능을 측정한 결과, 번데기를 이용한 고체배양보다 액체배양에서 더 높은 전자공여능을 나타내었다.

Abstract

The effects of liquid culture conditions and nutrient sources on the formation of protein-binding polysaccharide (PS) in Cordyceps militaris were examined. The formation amount of PS was increased in proportion to the growth rate of mycelium, in case of higher aeration or lower acidity. The optimum growth temperature of the mycelia was 25$^{\circ}C$ for the formation of PS. The optimum carbon source and nitrogen source were glucose and peptone, respectively. The ratio of C/N was optimal with 3% glucose to 0.5 % peptone. The sugar composition in the PS was greatly changed according to the carbon sources. The mycelium of Cordyceps militaris by liquid culture showed a higher electron donating ability than that by solid culture.

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이 논문을 인용한 문헌 (2)

  1. Kang, Bok-Hee ; Lee, Sang-Han ; Hur, Sang-Sun ; Shin, Yong-Kyu ; Lee, Dong-Sun ; Chang, Hung-Bae ; Song, Bong-Jun ; Lee, Jin-Man 2010. "The Cultural Characteristics of Paecilomyces tenuipes" 한국식품저장유통학회지 = Korean journal of food preservation, 17(3): 365~369 
  2. Park, Byung-Sung 2011. "Effect of Feeding Cordyceps with Fly Pupa on Growth Performance in Broiler Chickens" 생명과학회지 = Journal of life science, 21(11): 1541~1548 

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