Exposure to stress is known to precipitate or exacerbate many reproductive dysfunctions such as dysmenorrhea and infertility. Abnormalities of the reproductive system, as shown by reduced ovulation, fertilization and early embryonic development, are frequently seen in dysmenorrhea and infertility. I...
Exposure to stress is known to precipitate or exacerbate many reproductive dysfunctions such as dysmenorrhea and infertility. Abnormalities of the reproductive system, as shown by reduced ovulation, fertilization and early embryonic development, are frequently seen in dysmenorrhea and infertility. It has been generally accepted that Gamisoyosan (GSS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also GSS has been used traditionally to improve systemic circulation and biological energy production. Based on these, this study investigates whether GSS improved ovarian dysfunction caused by stress in mice. Mice were subjected to stress by electric shock on the foot for 30 min daily for a week and treated with GSS at 500 / body weight per day for one week. Thereafter, changes body weight, adrenal weight, ovulation rate, in vitro and in vivo fertilization, embryonic development and estradiol concentrations were measured. GSS markedly increased the body weight of mice with stress, but not normal mice. The administration of GSS caused a reduction in adrenal weight in stressed mice. GSS also had significant positive effects on ovulation rate, estradiol production, in vivo and in vitro fertilization rates and embryonic development. These results indicate that GSS can improve the reproductive dysfunctions caused by stress, and these may production biological energy.
Exposure to stress is known to precipitate or exacerbate many reproductive dysfunctions such as dysmenorrhea and infertility. Abnormalities of the reproductive system, as shown by reduced ovulation, fertilization and early embryonic development, are frequently seen in dysmenorrhea and infertility. It has been generally accepted that Gamisoyosan (GSS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also GSS has been used traditionally to improve systemic circulation and biological energy production. Based on these, this study investigates whether GSS improved ovarian dysfunction caused by stress in mice. Mice were subjected to stress by electric shock on the foot for 30 min daily for a week and treated with GSS at 500 / body weight per day for one week. Thereafter, changes body weight, adrenal weight, ovulation rate, in vitro and in vivo fertilization, embryonic development and estradiol concentrations were measured. GSS markedly increased the body weight of mice with stress, but not normal mice. The administration of GSS caused a reduction in adrenal weight in stressed mice. GSS also had significant positive effects on ovulation rate, estradiol production, in vivo and in vitro fertilization rates and embryonic development. These results indicate that GSS can improve the reproductive dysfunctions caused by stress, and these may production biological energy.
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문제 정의
Rensis and Scaramuzzi (2003) studied there is a reduction in LH secretion leading to reduced estrogen secretion, impaired detection of estrus, reduced oocyte quality, implantation failure and infertility. In terms of these suggestions, this study examined the effects of GSS on fertilization rate and embryonic development. GSS conspicuously improved the rates of in vitro and in vivo fertilization and embryonic development in stressed mice (Tables VI and VII).
가설 설정
In order to this, the present study investigates whether GSS will affect the ovulation rate, both the in vivo and in vitro fertilization rates and embryonic development in mice subjected to stress.
제안 방법
After treating mice with or without GSS for a weeks, in vivo fertilization and the rate of embryonic development to the blastocyst stage were examined (Table VI). In mice given stress, in vivo fertilization rates in GSS-treated mice were significantly higher compared with those of control mice, being 936%, pvO.
After treating mice with or without GSS for a we아<s, in vitro fertilization and the rate of embryonic development to the blastocyst stage were examined (Table VII). In mice exposed to stress, in v/Yro fertilization rates in GSS-treated mice were significantly higher compared with those of control mice, being 86.
The present study was designed to clarify the biological efficacy of GSS on the abnormalities of the female reproductive system using mice with stress induced by electric shock. In order to this, the present study investigates whether GSS will affect the ovulation rate, both the in vivo and in vitro fertilization rates and embryonic development in mice subjected to stress.
대상 데이터
Each component of GSS is listed in Table I. All materials were obtained from the College of Oriental Medicine, Wonkwang University. This prescription was prepared according to the method by Yu et al.
성능/효과
These resuIts suggest that GSS has a therapeutic effect on the abnormalities of the neuroendocrine system caused by acute stress. The findings that GSS did not affect the adrenal weight or the glucocorticoid feedback in the normal mice indicate that this drug does not positively decrease the adrenal weight or accelerate the feedback, suggesting that this drug is effective against abnormalities of the neuroendocrine system that occur specifically under stressfu conditions.
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