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논문 상세정보

Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite, which invades a wide range of hosts including humans. The exact mechanisms involved in its invasion are not fully understood. This study focused on the roles of $Ca^{2+}$ in host cell invasion and in T. gondii replication. We examined the invasion and replication of T. gondii pretreated with several calcium modulators, the conoid extrusion of tachyzoites. Calmodulin localization in T. gondii were observed using the immunogold method, and $Ca^{2+}$ levels in tachyzoites by confocal microscopy. In light microscopic observation, tachyzoites co-treated with A23187 and EGTA showed that host cell invasion and intracellular replication were decreased. The invasion of tachyzoites was slightly inhibited by the $Ca^{2+}$ channel blockers, bepridil and verapamil, and by the calmodulin antagonist, calmidazolium. We observed that calcium saline containing A23187 induced the extrusion of tachyzoite conoid. By immunoelectron microscopy, gold particles bound to anti-calmodulin or anti-actin mAb, were found to be localized on the anterior portion of tachyzoites. Remarkably reduced intracellular $Ca^{2+}$ was observed in tachyzoites treated with BAPTA/AM by confocal microscopy. These results suggest that host cell invasion and the intracellular replication of T. gondii tachyzoites are inhibited by the calcium ionophore, A23187, and by the extracellular calcium chelator, EGTA.

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이 논문을 인용한 문헌 (5)

  1. Jiang, Jing-Hua ; Kim, Hwa-Kyoung ; Kim, Chun-Mei ; Jo, Hyang-Jeong ; Kim, Hun-Soo ; Yun, Ki-Jung ; Park, Hyun 2007. "Anti-Toxoplasma Activity of Traditional Medicine Against Toxoplasma. gondii in vitro" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 21(2): 511~513 
  2. Jiang, Jing-Hua ; Kim, Hwa-Kyoung ; Kim, Hye-Sook ; Kamata, Masaki ; Wataya, Yusuke ; Park, Hyun 2007. "1, 5 - bis (4 - methoxyphenyls) - 6, 7 - Dioxa-bicyclo [3.2.2] nonane's Anti-toxoplasmosis Effect that was Separated in Artemisin annula" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 21(1): 82~85 
  3. Kim, Hye-Kung ; Jiang, Jing-Hua ; Lee, Dong-Hwan ; Kim, Hye-Sook ; Park, Hyun 2008. "Anti-Toxoplasmosis Effect of Citrus Unshiu Markovich against Toxoplasma Gondii" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 22(1): 96~99 
  4. Hong, Sunhwa ; Lee, Hyun-A ; Chung, Yung-Ho ; Kim, Tae-Wan ; Kim, Okjin 2014. "Anti-Toxoplasmosis effects of herbal extracts against Toxoplasma Gondii" 韓國家畜衛生學會誌 = Korean journal of veterinary service, 37(2): 101~104 
  5. Hong, Sunhwa ; Lee, Hyun-A ; Lee, Yun-Seong ; Kim, Dong-Woo ; Jeong, Jae-Hyeok ; Kim, Tae-Wan ; Kim, Okjin 2014. "Anti-Toxoplasmosis Effect of the Halophyte Suaeda maritime" 韓國資源植物學會誌 = Korean journal of plant resources, 27(5): 415~420 

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