1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided i...
1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided into 2 groups, with thirteen mice each one, as ad libitum group and as dietary restriction group (60% of ad libitum feeding amount). After feeding for a given period, the mice were sacrificed to get the following results: among the experimental groups, there wereno significant differences in xanthine oxidase (XOD) activity in their kidney tissues. The contents of cytochrome $P_{450}$ decreased in ad libitum group and dietary restriction group by age. The activity of NADPH-cytochrome $P_{450}$ reductase showed a trend similar to cytochrome $P_{450}$. Superoxide radical content increased with age. At the 4th, 8th and 12 months of the experimental period, the activity in the dietary restriction group was less than that of ad libitum group by as much as 17% 14% and 14% respectively. For hydrogen peroxide, the contents were increased in the ad libitum group with age, while no correlation between content and age was observed in the dietary restriction group. In the 8th and 12th months of the experimental period, the were in the dietary restriction group less than that of ad libitum group counterpart as much as 17% and 20o/c, respectively. For the cellular membrane stability of the kidney, no significant correlation with age was observed in either the dietary restriction group or the ad libitum group. However at the 12th month of the experiment, however, the stability in the dietary restriction group was 11 % higher than that in the ad libitum group. In conclusion, with these results obtained from the SAMP8 mouse model, we demonstrate that dietary restriction has the effects of anti-oxidation and anti-senescence in the kidney.
1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided into 2 groups, with thirteen mice each one, as ad libitum group and as dietary restriction group (60% of ad libitum feeding amount). After feeding for a given period, the mice were sacrificed to get the following results: among the experimental groups, there wereno significant differences in xanthine oxidase (XOD) activity in their kidney tissues. The contents of cytochrome $P_{450}$ decreased in ad libitum group and dietary restriction group by age. The activity of NADPH-cytochrome $P_{450}$ reductase showed a trend similar to cytochrome $P_{450}$. Superoxide radical content increased with age. At the 4th, 8th and 12 months of the experimental period, the activity in the dietary restriction group was less than that of ad libitum group by as much as 17% 14% and 14% respectively. For hydrogen peroxide, the contents were increased in the ad libitum group with age, while no correlation between content and age was observed in the dietary restriction group. In the 8th and 12th months of the experimental period, the were in the dietary restriction group less than that of ad libitum group counterpart as much as 17% and 20o/c, respectively. For the cellular membrane stability of the kidney, no significant correlation with age was observed in either the dietary restriction group or the ad libitum group. However at the 12th month of the experiment, however, the stability in the dietary restriction group was 11 % higher than that in the ad libitum group. In conclusion, with these results obtained from the SAMP8 mouse model, we demonstrate that dietary restriction has the effects of anti-oxidation and anti-senescence in the kidney.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
This study investigated the activities of free radical production system and phases of free radical production to examine the effect of calorie-controlled diet on aging in kidney tissues. SAMP8, an anim시 widely used in aging studies, were divided into two groups as AL (ad libitum) and DR (dietary restriction [fed with 60% of AL]) and we examined the effect of calorie restriction at 4th, 8th, 12th month and at death.
이론/모형
Mitochondria, a compartment from the kidney tissue and membrane fluidity, were measured according to the method of Heron et al. using 1, 6-diphenyl-1, 3, 5-hexatrine (DPH) as fluorescent probe. Using fluorescence spectro meter, the fluidity was measured at 360 nm of excitation wave length and 430 nm of emission wave length by adding and mixing 50 mM phosphate buffered solution (pH 7.
성능/효과
and reproduction of cells. Considering the effect of DR on the stability of cell membrane obtained from the kidney tissues fraction, using 1, 6-diphenyl-l, 3, 5-hexatriene (DPH) as a probe (Fig. 6), there were no significant changes in all ages of both AL and DR groups (p<0.05), but it was indicated that it was higher in DR group than in AL group; especially, it increased about 11% in the 12 month, without significant differences.
The results of activities of NADPH-cytochrome P450 reductase observation, that consists of each FAD and FMN molecule and functions as a catalyst to transport electron from NADPH to cytochrome P450 in the kidney (Fig. 3), showed that the activities were similar to these of cytochrome P450 and they were significantly reduced by age increase in the AL group. However, there were no significant differences by age in the DR group.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.