$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

In order to better utilize insertional mutagenesis and functional genomics in Chinese cabbage, we have developed an improved transformation system that more efficiently produces a large number of transgenic plants. Hypocotyl explants were inoculated with Agrobacterium tumefaciens LBA4404. This strain harbors tagging vector pRCV2, which contains a hygromycin-resistance gene, an ampicillin resistance gene, and a bacterial replication origin within the T-DNA. Transformation efficiency was highest when the explants were first co-cultivated for 3 d in a medium supplemented with 5 mg L-1 acetosyringone, then transferred to a 0.8% agar selection medium containing 10 mg L-1 hygromycin. In addition, maintaining a low pH in the co-cultivation medium was critical to enhancing transformation frequency. A total of 3369 transgenic plants were obtained, with efficiencies ranging from 2.89% to 5.00%. Southern blot analysis and T1 progeny tests from 120 transgenic plants confirmed that the transgenes were stably inherited to the next generation. We also conducted plasmid rescue and inverse PCR with some transformants, based on their phenotype, to demonstrate the applicability of T-DNA tagging in Chinese cabbage. The tagged sequences were then analyzed.

참고문헌 (20)

  1. Feldmann KA, Malmberg R, Dean C (1994) In Arabidopsis. E. Meyerowitz, C. Somerville, eds. Cold Spring Harbor Laboratory Press, pp 137-172 
  2. Mukhopadhyay A, Arumugam N, Nandakumar PBA, Pradhan AK, Gupta V, Pental D. (1992) Agrobacterium-mediated genetic transformation of oilseed Brassica campestris: transformation frequency is strongly influenced by the mode of shoot regeneration. Plant Cell Rep 11: 506-513 
  3. Cooley MB, Goldsbrough AP, Still DW, Yoder JI (1996) Site-selected insertional mutagenesis of tomato with maize Ac and Ds elements. Mol Gen Genet 252: 184-194 
  4. Spradling AC, Stern DM, Kiss I, Roote J, Laverty T, Rubin GM (1995) Gene disruptions using P transposable ele-ments: An integral component of the Drosophila genome project. Proc Natl Acad Sci USA 92: 10802-10803 
  5. Cho HS, Cao J, Ren JP, Earle ED (2001) Control of Lepi-dopteran insect pests in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) transformed with a syn-thetic Bacillus thuringiensis sry1C gene. Plant Cell Rep 20:1-7 
  6. Frey M, Stettner C, Gierl A (1998) A general method for gene isolation in tagging approaches: amplification of insertion mutagenized sites (AIMS). PIant J 13: 717-721 
  7. Liu YG, Whittier RF (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chro-mosome walking. Genomics 25: 674-681 
  8. Christey MC, Sinclair BK, Braun RH (1997) Regeneration of transgenic vegetable brassicas (Brassica oleracea and B. campestris) via Ri-mediated transformation. Plant Cell Rep 16: 587-593 
  9. Rohini VK, Rao KS (2000) Transformation of peanut (Ara-chis hypogaea L.): a non-tissue culture based approach for generating transgenic plants. Plant Sci 150: 41-49 
  10. Koes R, Souer E, van Houwelingen A, Mur L, Spelt C, Quattrocchio F, Wing J, Oppedijk B, Ahmed S, Maes T, Gerats T, Hoogeveen P, Meesters M, Kloos D (1995) Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants. Proc Natl Acad Sci USA 92: 8149-8153 
  11. Zhang FL, Takahata Y, Xu JB (1998) Medium and genotype factors influencing shoot regeneration from cotyledon-ary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 17: 780-786 
  12. Lee MK, Kim HS, Park YD (2004) Analysis of T-DNA inte-gration patterns in transgenic tobacco plants. J Plant Biol 47: 179-186 
  13. Takasaki T, Hatakeyama K, Ojima K, Watanabe M, Toriyama K, Hinata K (1997) Factors influencing Agro-bacterium-mediated transformation of Brassica rapa L. Breed Sci 47: 127-134 
  14. An G, Ebert PR, Mitra A, Ha SB (1988) Binary vectors, In SB Gelvin, RA Schilperoort, DPS Verma, eds, Plant Molecular Biology Mannual, A3. Academic Publishers, Dordrecht, pp 1-19 
  15. Zhang FL, Takahata Y, Watanabe M, Xu JB (2000) Agrobac-terium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinen-sis). Plant Cell Rep 19: 569-575 
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473-497 
  17. Krysan PJ, Young JC, Sussman MR (1999) T-DNA as an insertional mutagen in Arabidopsis. Plant CeII 11: 2283-2290 
  18. Jun SI, Kwon SY, Paek KY, Paek KH (1995) Agrobacterium-mediated transformation and regeneration of fertile transgenic plants of Chinese cabbage (Brassica campes-tris L. ssp. pekinensis cv. 'Spring Flavor'). Plant Cell Rep 14: 620-625 
  19. Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, Doutriaux MP (1999) Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis. Plant Cell 11: 1623-1634 
  20. Kim HS, Kim SH, Park YD (2003) Development of rescue cloning vector with phosphinothricin resistant gene for effective T-DNA tagging. Kor J Hort Sci 18: 594-598 

이 논문을 인용한 문헌 (4)

  1. Yu, Jae-Gyeong ; Lee, Gi-Ho ; Lim, Ki-Byung ; Hwang, Yoon-Jung ; Woo, Eun-Taek ; Kim, Jung-Sun ; Park, Beom-Seok ; Lee, Youn-Hyung ; Park, Young-Doo 2010. "Analysis of Mutant Chinese Cabbage Plants Using Gene Tagging System" 원예과학기술지 = Korean journal of horticultural science & technology, 28(3): 442~448 
  2. Yu, Jae-Gyeong ; Park, Ji-Hyun ; Park, Young-Doo 2010. "Current status of Brassica rapa functional genome research in Korea" Journal of plant biotechnology = 식물생명공학회지, 37(2): 166~173 
  3. 2010. "" Molecules and cells, 29(3): 267~275 
  4. Yu, Jae-Gyeong ; Lee, Gi-Ho ; Park, Young-Doo 2012. "Comparison of RNA Interference-mediated Gene Silencing and T-DNA Integration Techniques for Gene Function Analysis in Chinese Cabbage" 원예과학기술지 = Korean journal of horticultural science & technology, 30(6): 734~742 

원문보기

원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

이 논문과 연관된 기능

DOI 인용 스타일