$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Molecular Characterization of a cDNA for a Cysteine-Rich Antifungal Protein from Capsicum annuum

Journal of plant biology = 식물학회지, v.47 no.4, 2004년, pp.375 - 382  

Lee, Yeon-Mi (Department of Biological Sciences, Seoul National University) ,  Wee, Hyoung-Suk (Department of Biological Sciences, Seoul National University) ,  Ahn, Il-Pyung (School of Agricultural Biotechnology and Center for Agricultural Biomaterials) ,  Lee, Yong-Hwan (School of Agricultural Biotechnology and Center for Agricultural Biomaterials) ,  An, Chung-Sun (Department of Biological Sciences, Seoul National University)

Abstract AI-Helper 아이콘AI-Helper

We have isolated a cDNA clone for the antifungal protein, CaAFP, from hot pepper, Capsicum annuum L. Its open reading frame encodes 85 amino acids, including 8 cysteine residues. CaAFP consists of three domains: a signal peptide, a chitin-binding domain, and a C-terminal peptide domain. The deduced ...

주제어

참고문헌 (32)

  1. J Mol Biol SF Altschul 215 403 1990 10.1016/S0022-2836(05)80360-2 Altschul SF, Gish W, Miller W, Myers EW, Lipman D (1990) Basic local alignment search tool. J Mol Biol 215:403-410 

  2. J Rubber Res Inst Malaysia BL Archer 21 560 1969 Archer BL, Audley BG, Sweeney GP, Hong TC (1969) Studies on composition of latex serum and bottom fraction particles. J Rubber Res Inst Malaysia 21: 560 

  3. FM Ausubel 451 1987 Current Protocols in Molecular Biology Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current Protocols in Molecular Biology. John Wiley & Sons, New York, pp 451-453 

  4. Science D Benton 196 180 1977 10.1126/science.322279 Benton D, Davis RW (1977) Screening lambda-gt recombinant clones by hybridization to single plaquesin situ. Science 196: 180-182 

  5. Anal Biochem MM Bradford 72 248 1976 10.1016/0003-2697(76)90527-3 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 

  6. Proc Natl Acad Sci USA WF Broekaert 87 7633 1990 10.1073/pnas.87.19.7633 Broekaert WF, Lee HI, Kush A, Chua NH, Raikhel N (1990) Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis). Proc Natl Acad Sci USA 87:7633-7637 

  7. Plant Physiol WF Broekaert 108 1353 1995 10.1104/pp.108.4.1353 Broekaert WF, Terras FRG, Cammue BPA, Osborn RW (1995) Plant defensins: Novel antimicrobial peptides as components of the host defense system. Plant Physiol 108:1353-1358 

  8. Plant Cell MJ Chrispeels 3 1 1991 10.1105/tpc.3.1.1 Chrispeels MJ, Raikhel NV (1991) Lectins, lectin genes, and their role in plant defense. Plant Cell 3:1-9 

  9. Mol Plant Microbe Interact F Cote 4 173 1991 10.1094/MPMI-4-173 Cote F, Cutt JR, Asselin A, Klessig DF (1991) Pathogenesisrelated acidic beta-1, 3 glucanase genes of tobacco are regulated by both stress and developmental signals. Mol Plant Microbe Interact 4:173-181 

  10. KH Cox 1 1988 Plant Molecular Biology: A Practical Approach Cox KH, Goldberg RG (1988) Analysis of plant gene expression,In CH Shaw, ed, Plant Molecular Biology: A Practical Approach. IRL Press, Oxford, pp 1-35 

  11. Plant Mol Biol MFC Bolle De 22 1187 1993 10.1007/BF00028991 De Bolle, MFC, David KMM, Sarah BR, Vanderleyden J, Cammue BPA, Broekaert WF (1993) Cloning and characterization of a cDNA encoding an antimicrobial chitin-binding protein from amaranth,Amaranthus caudatus. Plant Mol Biol 22: 1187-1190 

  12. Plant Mol Biol MFC Bolle De 28 713 1995 10.1007/BF00021195 De Bolle MFC, Eggermont K, Duncan RE, Osborn RW, Terras FRG, Broekaert WF (1995) Cloning and characterization of two cDNA clones encoding seed-specific antimicrobial peptides fromMirabilis jalapa L. Plant Mol Biol 28:713-721 

  13. Plant Mol Biol MFC Bolle De 31 993 1996 10.1007/BF00040718 De Bolle MFC, Osborn RW, Goderis IJ, Noe L, Acland D, Hart CA, Torrekens S, van Leuven F, Broekaert WF (1996) Antimicrobial peptides fromMirabilis jalapa andAmaranthus caudatus: Expression, processing, localization and biological activity in transgenic tobacco. Plant Mol Biol 31:993-1008 

  14. Biopoly J-L Dimarcq 47 465 1998 10.1002/(SICI)1097-0282(1998)47:6<465::AID-BIP5>3.0.CO;2-# Dimarcq J-L, Philippe B, Charles H, Jules H (1998) Cysteinerich antimicrobial peptides in invertebrates. Biopoly 47:465-477 

  15. Biopoly F Garcia-Olmedo 47 479 1998 10.1002/(SICI)1097-0282(1998)47:6<479::AID-BIP6>3.0.CO;2-K Garcia-Olmedo F, Molina A, Josefa M, Alamillo R-PP (1998) Plant defense peptides. Biopoly 47:479-491 

  16. Protein Express Purific SJ Harrison 15 171 1999 10.1006/prep.1998.0992 Harrison SJ, McManus AM, Marcus JR Goulter KC, Green JL, Nielsen KJ, Craik DJ, Maclean DJ, Manners JM (1999) Purification and characterization of a plant antimicrobial peptide expressed inEscherichia coli. Protein Express Purific 15: 171-177 

  17. CABIOS DG Higgins 8 189 1992 Higgins DG, Bleasby AJ, Fuchs R (1992) Clustal V: Improved software for multiple sequence alignment. CABIOS 8:189-191 

  18. Z Pflan-zenkr Pflanzen-schutz J Horsfall 64 415 1957 Horsfall J, Dimond A (1957) Interactions of tissue sugar, growth substances and disease susceptibility. Z Pflan-zenkr Pflanzen-schutz 64:415-421 

  19. Mol Cells SR Kim 6 753 1996 10.1016/S1016-8478(23)10918-6 Kim SR, Kim SA, Kwak HJ (1996) Isolation and characterization of hot pepper calmodulin cDNA clone. Mol Cells 6:753-758 

  20. Koo JC, Lee SY, Chun HJ, Park SH, Lee BL, Cho MJ (1995) Sequential expression of two identical hevein homologs with a potent antifungal activity during maturation and germination of the seeds ofPharbitis nil. Presented at the 4th International Workshop on Pathogenesis-Related Proteins in Plants: Biology and Biotechnological Potential, Kloster Irsee, Germany 

  21. Biochim Biophys Acta JC Koo 1382 80 1998 10.1016/S0167-4838(97)00148-9 Koo JC, Lee SY, Jin CH, Hwa CY, Choi JS, Kawabata S-I, Masaru M, Susumu T, Kwon SH, Bae DW, Han CD, Lee BL, Cho MJ (1998) Two hevein homologs isolated from the seed ofPharbitis nil L. exhibit potent antifungal activity. Biochim Biophys Acta 1382:80-90 

  22. J Biol Chem HI Lee 266 15944 1991 10.1016/S0021-9258(18)98499-1 Lee HI, Broekaert WF, Raikhel NV (1991) Co- and postradiational processing of the hevein preproprotein in latex of the rubber tree (Hevea brasiliensis). J Biol Chem 266:15944-15948 

  23. Plant Cell T Lotan 1 881 1989 10.1105/tpc.1.9.881 Lotan T, Ori N, Fluhr R (1989) Pathogenesis-related proteins are developmentally regulated in tobacco flowers. Plant Cell 1:881-887 

  24. HI McKhann 179 1993 Methods in Plant Molecular Biology and Biotechnology McKhann HI, Hirsch AM (1993)In situ localization of specific mRNAs in plant tissues.In BR Click, JE Thompson, eds, Methods in Plant Molecular Biology and Biotechnology. CRC Press, Boca Raton, pp 179-205 

  25. Plant Physiol B Meyer 112 615 1996 10.1104/pp.112.2.615 Meyer B, Houlne G, Pozueta-Romero J, Schantz ML, Schantz R (1996) Fruit specific expression of a defensintype gene family in bell pepper: Upregulation during ripening and upon wounding. Plant Physiol 112:615-622 

  26. Plant Physiol KK Nielsen 113 83 1997 10.1104/pp.113.1.83 Nielsen KK, Nielsen JE, Madrid SM, Mikkelsen JD (1997) Characterization of a new antifungal chitin-binding peptide from sugar beet leaves. Plant Physiol 113:83-91 

  27. Plant Mol Biol BJ Oh 41 313 1999 10.1023/A:1006336203621 Oh BJ, Ko MK, Kostenyuk I, Shin BC, Kim KS (1999) Coexpression of a defensin gene and a thionin-like gene via different signal transduction pathways in pepper andColletotrichum gloeosporioides interaction. Plant Mol Biol 41:313-319 

  28. Plant Physiol RA Salzman 117 463 1998 10.1104/pp.117.2.465 Salzman RA, Tikhonova I, Bordelon BR Hasegawa PM, Bressan RA (1998) Coordinated accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defense response during fruit ripening in grape. Plant Physiol 117:463-472 

  29. Proc Natl Acad Sci USA F Sanger 74 5463 1977 10.1073/pnas.74.12.5463 Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467 

  30. Appl Environ Microbiol M Schirmbock 60 4364 1994 10.1128/AEM.60.12.4364-4370.1994 Schirmbock M, Lorito M, Wang YL, Hayes CK, Arisan-Atac I, Scala F, Harman GE, Kubicek CP (1994) Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action ofTrichoderma harzianum against phytopathogenic fungi. Appl Environ Microbiol 60:4364-4370 

  31. J Biol Chem FRG Terras 267 15301 1992 10.1016/S0021-9258(19)49534-3 Terras FRG, Schoofs HME, De Bolle MFC, van Leuven F, Rees SB, Vanderleyden J, Cammue BRA, Broekaert WF (1992) Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 267:15301-15309 

  32. Planta J Parijs van 183 258 1991 10.1007/BF00197797 van Parijs J, Broekaert WF, Goldstein IJ, Peumans WJ (1991) Hevein: An antifungal protein from rubber-tree (Hevea brasiliensis) latex. Planta 183:258-264 

저자의 다른 논문 :

관련 콘텐츠

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로