[논문]Characterization of Korean Erwinia carotovora Strains from Potato and Chinese Cabbage

Seo, Sang-Tae; Koo, Jun-Hak; Hur, Jang-Hyun; Lim, Chun-Keun

doi:10.5423/ppj.2004.20.4.283

Characterization of Korean Erwinia carotovora Strains from Potato and Chinese Cabbage 원문보기

The plant pathology journal, v.20 no.4, 2004년, pp.283 - 288

Seo, Sang-Tae (Division of Horticultural Environment, Natinal Horticultural Research Institute, RDA.) , Koo, Jun-Hak (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) , Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) , Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)

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Four Erwinia carotovora strains isolated from potatoes showing blackleg symptoms and rotted Chinese cabbage were analysed by biochemical tests and sequence analysis of 16S rDNA and 16S-23S rRNA intergenic spacer (IGS) regions, and the data were compared to related E. carotovora strains. Based on the results of the biochemical tests and sequence analysis, 2 of the 4 strains were identified as E. carotovora subsp. carotovora (Ecc), whereas the rest strains were distinct from Ecc. The last two strains, HCC3 and JEJU, were biochemically similar to E, carotovora subsp. atroseptica (Eca). However, the results of sequence analysis and Eca-specific PCR assays showed that the strains were distinct from Eca. On the basis of 16S rDNA sequence analysis, HCC3 and JEJU strains were placed in E. carotovora subsp. odorifera and E. carotovora subsp. wasabiae, respectively. The results of sequence analysis and specific PCR assay for Eca indicated that Asian Eca strains were distinct from European Eca strains, although they were phenotycally homogeneous.

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(2000) reported that seq_u ing of the 690 bp product, generated by PCR using primers ECAIf and ECA2r revealed similarity to a sequence in Escherichia coll encoding formate acetyltransferase. We hypothesize that the sequence of its gene in Asian Eca strains are different from those of European Eca strains.

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, 1991). Amplification was performed in a total volume of 50㎕ containing 20 pmol each primer, 200 mM of mixture from dATP, dCTP, dGTP, dTTP (Promega, Southampton, England), 2 mM MgCl2, 1 x buffer, template DNA (ca. 20 ng) and 2.5 units of Taq polymerase (Promega), under the following reaction conditions; 94℃ for 4 min for initial denaturation, 35 cycles of 94℃ for 1 min, 58℃ for 1 min and 72℃ for 3 min, followed by a final elongation step of 72℃ for 10 min. The 16S-23S rRNA intergenic spacer (IGS) regions were amplified by using primers R16-1F and R23-1R (Nakagawa et al, 1994).
However, the characteristics of the rest strains (JEJU and HCC₃) were similar to those of Eca reference strains which did not grow at 36℃ (Table 2). On the basis of Eca-specific PCR assays, the both strains produced no PCR product in contrast to the single 690 bp amplicon obtained with three European Eca strains tested(Fig. 1). In addition, two Japanese Eca strains (MAFF 301629 and MAFF 301630) and Korean Eca strain (HCP1) were not amplified under the conditions used.
Purified DNAs were ligated into pGEM-T easy vector (Promega). Plasmids containing the 16S rDNA and IGS regions were then sequenced directly by cycling sequencing using an ALFred autocycle sequencing kit with M13 forward and reverse primers.

성능/효과

7%), respectively. As expected, the dendrogram of distances indicated that European Eca strains were genotypically distant from Asian Eca strains based on comparisons of the small and large 16S-23S rDNA sequences (Fig. 3).
investigated were identified as Ecc. However, the other two strains JEJU and HCC₃ were not clearly defined in this study, because they showed a mixture of Eca, Eco and Ecw phenotypic and genotypic characteristics. We believe that a more intensive survey would have revealed a greater occurrence of these bacteria.
The 16S rDNA sequence of the four strains was consistent with its identity as a member of the E. carotovora species (Fig. 2). Results of 16S rDNA sequence analysis showed high similarity between HCC₃ and Eco reference strains.

참고문헌 (16)

Asai, T., Zaporojets, D., Squires, C. and Squires, C. L. 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchangeof rRNA genes between bacteril. Proc. Natl. Acad. Sci. US.A. 96:1971-1976

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Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidmann, J. G, Smith, J. A. and Struhl. K. 1987. Current protocols in molecular biology. Greene Publishing Associates and Wiley Interscience, N. Y
Condon, C., Squires, C. and Squires, C. L. 1995. Control of rRNA transcription in Escherichia coli. Microbiol. Rev. 59:623-645

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Dallagi, A., Birch, P. R. J., Heilbronn, J., Lyon, G and Toth, I. K. 2000. cDNA-AFLP analysis of differential gene expression in the bacterial plant pathogen Envinia carotovora. Microbiology 146:165-171
Darrasse, A., Priou, S., Kotoujansky, A. and Bertheau, Y. 1994. PCR and restriction length polymorphism of a pel gene as a tool to identitY Erwinia carotovora in relation to potato diseases. Appl. Environ. Microbiol. 60: 1437-1443
De Boer, S. H. and Kelman, A. 2000. Gram-negative bacteria: Erwinia soft rot group. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, 3rd edn. ed. Schaad, N. W, Jones, J. B. and Chun, W pp. 56-72. St Paul, MN: American Phytopathological Society
De Boer, S. H. and Ward, L. J. 1995. PCR detection of Envinia carotovora subsp. atroseptica associated with potato tissue Phytopathology 85:854-858

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De Boer, S. H., Cuppels, D. A. and Kelman, A. 1978. Pectolytic Erwinia spp. in the root zone of potato plants in relation to infestation of daughter tubers. Phytopathology 68: 1784-1790

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Duarte, V., De Boer, S. H., Ward, L. J. and De Oliveira, A. M. R. 2004. Characterization of atypical Erwinia carotovora strains causing blackleg of potato in BraZil. J Appl. Microbiol. 96:535-545

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Fessehaie, A., De Boer, S. H. and Levesque, C. A. 2002. Molecular characterization of DNA encoding 16S-23S rRNA intergenic spacer regions and 16S rRNA of pectolytic Envinia species. Can. J Microbiol. 48:387-398

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Hauben, L., Moore, E. R. B., Vauterin, L., Steenackers, M., Mergaert, J., Verdonck, L. and Swings, J. 1998. Phylogenetic position of phytopathogens within the Enterobacteriaceae. System. Appl. Microbiol. 21:384-397

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Lelliot, R. A. and Dickey, R. S. 1984. Genus VII. Erwinia Winslow,Broadhurst, Buchanan, Krumwiede, Rogers and Smith1920, $209^A^L$ In Krieg, N. R., Holt, J.G eds, Bergey's manual of systematic bacteriology, vol. 1. Baltimore, USA
Nakagawa, T., Shimada, M., Mukai, H., Asada, K., Kato, I., Fuhino, K. and Sato, T. 1994. Detection of alcohol-tolerant Hiochi bacteria by PCR. Appl. Environ. Microbiol. 60:637-640

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Perombelon, M. C. M. and Salmond, G P.C. 1995. Bacterial soft rots, p. 1-17. In ingh, U. S., Singh, R. P. and Kohmoto, K. (ed.), Pathogenesis and host specificity in plant diseases, vol. 1. Pergamon Press, Oxford, England
Seo, S. T., Furuya, N., Lim, C. K. Lim, Y. and Tsuchiya, K. 2002. Phenotypic and genetic diversity of Erwinia carotovora ssp. carotovora strains from Asia. J Phytopathol. 150: 120-127

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Toth, I. K., Avrova, A. O. and Hyman, L. J. 2001. Rapid identification and differentiation of the soft rot Erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses. Appl. Environ. Microbiol. 67:4070-4076

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Characterization of Korean Erwinia carotovora Strains from Potato and Chinese Cabbage 원문보기

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