Kim, Young-Sook
(Major in Plant Pathology and Cell Technology, Department of Agricultural Biology, Chungnam National University)
,
Hwang, Eui-ll
(Bio Research Group, KT&G Central Research Institute)
,
O, Jeong-Hun
(Bio Research Group, KT&G Central Research Institute)
,
Kim, Kab-Sig
(Bio Research Group, KT&G Central Research Institute)
,
Ryu, Myong-Hyun
(Bio Research Group, KT&G Central Research Institute)
During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on...
During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$g/ml.
During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$g/ml.
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문제 정의
These tests led to the finding that culture filtrate of a bacterial isolate, KTB3, showed veiy high antiviral activity with systemic effects. This paper describes the inhibitory activity of culture filtrate from KTB3 on TMV CMY and PVY infection on tobacco plant.
대상 데이터
Two and three weeks after inoculation, systemic mosaic symptom appearances were examined and compared with the control with no treatment of the culture filtrate. The 90 plants were tested for each treatment with 3 replications.
이론/모형
Antiviral activity in local lesion host was tested by using the half-leaf method. The culture filtrate was applied with cotton swabs on the upper or underside surface of half leaf while dis-tilled water was applied on the remaining half as control.
성능/효과
Control of TMV in field. With treatment of the culture filtrate, TMV incidence was remarkably decreased, with 92.2% (Table 6) control efficacy at 2 weeks after inoculation. The control efficacy persisted above 91% up to 3 weeks after treatment.
참고문헌 (10)
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