The objectives were to compare the salt tolerance levels in the parental rice cultivar, Dongjinbyeo, and induced mutagenesis derived its lines for plant height, MDA, ATPase, POD, and 2-dimensional protein electrophoresis pattern in NaCl-containing hydroponic nutrient solutions. Rice plants isolated ...
The objectives were to compare the salt tolerance levels in the parental rice cultivar, Dongjinbyeo, and induced mutagenesis derived its lines for plant height, MDA, ATPase, POD, and 2-dimensional protein electrophoresis pattern in NaCl-containing hydroponic nutrient solutions. Rice plants isolated from a population of rice (Oryza sativa L. cv. Dongjinbyeo) mutation lines, which were generated in combination with in vitro selection and gamma-ray, exhibited salt tolerance. Line No. 18 had the longest plant, whereas NaCl-sensitive line (No. 25) had the shortest plant. The parent, and the sensitive line showed severe damage from salt stress. Tolerant lines (No. 18, 50) had a lower malonaldehyde (MDA) content than the sensitive one (Dongjinbyeo, No. 25) during salt stress. Several proteins showed significant quantitative variation through 2DE; phosphoribulokinase, peroxidase, oxygen evolving enhancer 1 and the $H^+-ATPase$, which are known to be involved in salt tolerance. The effect of salt on peroxidase and $H^+-ATPase$ activity in the seedlings of two groups with contrasting genotypes of rice was studied. A greater activity was recorded in the tolerant lines as compared to the sensitive ones (P<0.05, Duncan's test). The results indicate that salt tolerant lines expressed more salt stress-inducible proteins associated with salt tolerance than the sensitive lines during salt stress.
The objectives were to compare the salt tolerance levels in the parental rice cultivar, Dongjinbyeo, and induced mutagenesis derived its lines for plant height, MDA, ATPase, POD, and 2-dimensional protein electrophoresis pattern in NaCl-containing hydroponic nutrient solutions. Rice plants isolated from a population of rice (Oryza sativa L. cv. Dongjinbyeo) mutation lines, which were generated in combination with in vitro selection and gamma-ray, exhibited salt tolerance. Line No. 18 had the longest plant, whereas NaCl-sensitive line (No. 25) had the shortest plant. The parent, and the sensitive line showed severe damage from salt stress. Tolerant lines (No. 18, 50) had a lower malonaldehyde (MDA) content than the sensitive one (Dongjinbyeo, No. 25) during salt stress. Several proteins showed significant quantitative variation through 2DE; phosphoribulokinase, peroxidase, oxygen evolving enhancer 1 and the $H^+-ATPase$, which are known to be involved in salt tolerance. The effect of salt on peroxidase and $H^+-ATPase$ activity in the seedlings of two groups with contrasting genotypes of rice was studied. A greater activity was recorded in the tolerant lines as compared to the sensitive ones (P<0.05, Duncan's test). The results indicate that salt tolerant lines expressed more salt stress-inducible proteins associated with salt tolerance than the sensitive lines during salt stress.
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가설 설정
Several proteins were specific to one of the two lines (Triangles, Arrows). C. D: White rounds, rectangular and arrows indicate sequenced proteins, which were more induced in salt tolerant one than in the Dongjinbyeo. Black box and arrows mean the higher expressed proteins in the tolerant lines than in the Dongjinbyeo.
, 1994). The study demon strates that induced mutation holds to induce salt tolerance and elucidate the molecular mechanism of tolerance.
제안 방법
, 2000). For further detailed research, analysis of specific enzyme activity was carried out.
After three weeks of salini zation with 13 mS, salinity symptoms were scored according to the Standard Evaluation System (1-3: tolerant, 5: mo derate and 7-9: sensitive) developed at the IRRI. To re- estimate the salt tolerance, each thirty seeds of the tolerant and sensitive lines was identified in the first experiment, were placed in glass bottles (5 cm x 7 cm) with a salt-free solution and 0.75% salt treatment in these replicating and cultured for 30 days and 3 weeks, respectively. Plant height was used to assess the salt tolerance or sensitivity of the rice lines for the quantitative measurement of salt tolerance.
이론/모형
N-terminal sequence and the internal amino acid seque nces of the leaf protein were obtained from the Korea Basic Science Institute, Seoul Branch. Amino acid sequences were compared with the sequences in the Peptide Sequence Da tabase of the National Center for Biotechnology Information using the BLAST program.
The pellet was washed with the same extraction buffer and centrifuged in the same way. The protein in the extracts was quantified by the method of Bradford using BSA as the standard. For determining peroxidase activity, the reaction mixture (3 ml) consisted of 0.
참고문헌 (42)
Ahloowalia BS (1990) In vitro radiation induced mutagenesis in potato. In 'The Impact of Biotechnology in Agriculture'. Sangwan, R. S. and B. Sang wan-Norreel (eds.), Kluwer Acad Pub, The Netherlands p 39-46
Aragoncillo C., Rodriguez-Loperena M A, Salcedo G, Carbonero P, Garcia Olmedo F (1978) Influence of homeologus chromosomes on gene-doseage effects in allohexaploid wheat (Triticum aestivum). Proc Natl Acad Sci USA 75:1446-1450
Barriga PR, Fuentes P, Seemann P, Manquian N (1990) Variabilidad genetica de mutants de trigo en la absorcin y utilizacion de fosforo. Turrialba 40: 279-286
Burgos PA, Donaire JP (1996) $H^+$ -ATPase activities of tonoplast-enriched vesieles from non-treated and NaCI-treated jojoba roots. Plant Sci 118: 167-175
Claes B, Dekeyse R, Vinaroel R, Vanden Bulcke M, Bauw G, Van Montagu M, Caplan A (1990) Characterization of a rice gene showing organ-specific expression in response to salt stress and drought. Plant Cell 219-27
Chaudhuri K, Choudhuri MA (1993) Effects of short-term NaCI salinity stress on free radical mediated membrane damage in two jute species. Indian J Exp Bioi 31:327-331
Chen QF, Wang CL, Lu YM, Shen M, Afza R, Duren MV, Brunner H (2001) Anther culture in connection with induced mutations for rice improvement. Euphytica 120: 401-408
Damerval C, Ie Guilloux M, Blaisonneau J, de Vienne D (1987) A simplification of Heukeshoven and Dernick' s silver staining of proteins. Electrophoresis 8: 158-159
Damerval C, Maurice A, Josse JM, de Vienne D (1994) Quantitative traits loci underlying gene product variation: a novel perspective for analyzing regulation of genome expression. Genetics 137: 289-301
Das A, Gosal SS, Sidhu JS, Dhaliwal HS (2000) Induction of mutations for heat tolerance in potato by using in vitro culture and radiation. Euphytica 114: 205-209
Dhindsa R. Matowe SW (1981) Drought tolerance in two mosses corrected with enzymatic defense against lipid peroxidation. J Exp Bot 32: 79-91
Elstner EE (1987) Metabolism of activated oxygen species, in: D.D. Davies (eds.), The Biochemistry of Metabolism, Academic Press, San Diego, pp 253-315
Fiske CH, Subbarow YS (1952) The colorimetric determination of phosphorous. J. BioI. Chem. 66: 375-400
Garbarino J, DuPont FM (1989) Rapid induction of $Na^+$ / $H^+$ exchange activity in barley root tonoplast. Plant Physiol 89: 1-4
Gaspar T, Penel C, Hagage D, Greppin H (1991) Peroxidases in plant growth, differentiation and developmental processes, in: J.H. Lobarzewsky, H. Greppin, C Penel, T. Gaspar (eds.), Biochemical, Molecular, and Physiological aspects of Plant Peroxidases, University de Geneve, pp 249-280
Heath RL, Packer L (1968) Photoperoxidation in isolated chlorolasts. 1. Kinetics and stoichiometry of fattyacid peroxidation, Arch. Biochem. Biophys 125: 189-198
Hurkman W J, Tanaka CK (1986) Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol 81: 802-806
Kuiper PJC (1985) Environmental changes and lipid metabolism of higher plants. Physiol. Plant 64: 118-122
Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophaqe'Ia. Nature 227:680-685
Leonard R, Hotchkiss TCW (1976) Cation-stimulated adenosine triphosphate activity and cation transport in corn root. Plant Physio!. 58: 331-335
Lukaszewski A, Apolinarska B, Gustafson JP, Krolow KD (1994) Chromosome constitution of tetraploid triticale. Z Planzenzucht 93: 222-236
Maluszynski M, Ahloowalia BS, Sigurbjornsson B (1995) Application of in vitro mutation techniques for crop improvement. Euphytica 85: 303-315
Mansour M M F, Lee-Stadelmann OY, Stadelmann EJ (1993) Salinity stress and cytoplasmic factors. A comparison of cell permeability and lipid partiality in salt sensitive and salt tolerant cultivars and lines of Triticum aestivum and Hordeumvulgare. Physiol Plant 88: 141-148
Marais GF, Marais AS (1994) The derivation of compensating translocation involving homoelogus group 3 chromosomes of wheat and rye. Euphytica 79: 75-80
Median MI, Quesada MA, Pliego F, Botella MA, Valpuesta V (1999) Expression of the tomato peroxidase gene TPX1 in NaCI-adapted and unadaapted suspension cells. Plant Cell Rep 18: 680-683
Miao S, Duncan DR, Wilholm JM (1988) Selection of regenerable maize callus cultures resistant to 5-methyl-DL-trytophan, S-2-aminoethyl-L-cysteine and high level of L-Iysine plus L-threonine. Plant Cell Tiss Org Cult 14: 3-14
Moons A, Prinsen E, Bauw G, Montagu MV (1997) Antagonistic effects of abscisic acid and jasmonates on salt stressinducible transcripts in rice roots. The Plant Cell 9: 2243-2259
Morabito 0, Jolivet Y, Prat D. Dizengremel P (1996) Differences in the physiological responses of two clones of Eucalyptus microtheca selected for their salt tolerance. Plant Sci 114: 129-139
Morsomme P, Boutry M (2000) The plant plasma membrane $H^+$ -ATPase: structure, function and regulation. Biochimica et Biophysica Acta 1465: 1-16
Neto AT, de Oliveira Camargo CE, de Castro JL, Ferreira Fillo AWP (2001) Evaluation of Anahuac wheat mutant lines for aluminum tolerance. Euphytica 120: 339-343
Niu X, Bressan RA, Hasegawa PM, Pardo JM (1995). Ion homeostasis in NaCI stress environments. Plant Physiol 109: 735-742
O' Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Bio. Chem. 250: 4007-4021
Paul MJ, Driscoll SP, John Andralojc P, Knight JS, Gray JC, Lawlor OW (2000) Decrease of phosphoribulokinase activity by antisense RNA in transgenic tabacco: definition of the light environment under which phosphoribulokinase is not in large excess. Planta 211: 112-119
Pinet-Leblay C, Turpin FX, Chevreau S (1992) Effect of gamma and ultraviolet irradiation on adventitious regeneration from in vitro cultured pear leaves. Euphytica 62: 225-233
Raines CA, Longstaff M, Lloyd JC, Dyer TA (1989) Complete coding sequence of wheat phosphoribulokinase: developmental and light-dependent expression of the mRNA. Mol Gen Genet 1: 43-48
Reviron MP, Vartanian N, Sallantin M, Huet JC, Pernollet JC, Vienne D (1992) Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves. Plant Physiol 100: 1486-1493
Riccardi F, Gazeau P, de Vienne 0, Zivy M (1998) Protein changes in response to progressive water deficit in maize. Plant Physiol 117: 1253-1263
Sanada T (1986) Induced mutation breeding in fruit trees: resistant mutant to black spot disease of Japanese pear. Gamma field symposia 25: 87-108
Sreenivasulu L, Ramanjulu S, Ramaachandra-Kini K, Prakash HS, Shekar-Shetty H, Savithri HS, Shuhakar C (1999) Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance. Plant Sci 141: 1-9
Sugihara K, Hanagata N, Dubinsky Z, Baba S, Karube I (2000) Molecular characterization of cDNA encoding oxygen enolving enhancer protein 1 increased by salt treatment in the Mangrove Bruguiera gymnorrhiza. Plant Cell Physiol 41: 1279-1285
Tiwari BS, Bose A, Ghoshi B (1997) Photosynthesis in rice under a salt stress. PHOTOSYNTHETICA 34: 303-306
Watad A, Pesci EAPA, Reinhold L, Lerner HR (1986) Proton fluxes as a response to external salinity in wild type and NaCI-adapted Nicotiana cell lines. Plant Physiol 81: 454-459
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