[논문]Embryo Culture of Taxus wallichiana (Zucc.)

Datta Mukul Manjari; Jha Sumita

Embryo Culture of Taxus wallichiana (Zucc.) 원문보기

Journal of plant biotechnology, v.6 no.4, 2004년, pp.213 - 219

Datta Mukul Manjari (Centre of Advanced Study, Department of Botany, Calcutta University) , Jha Sumita (Centre of Advanced Study, Department of Botany, Calcutta University)

Abstract ▼ AI-Helper

Zygotic embryos were excised from immature and mature seeds of the Himalayan yew, Taxus wallichiana. The embryos germinated precociously when kept in darkness for 5 weeks and developed into full seedlings within 10-12 weeks. The highest rate of embryo germination ($81\%$) was obtained in modified Lloyd & McCown' s woody plant medium containing macro and micronutrients at half strength supplemented with $1\%$ activated charcoal, which supported both the best embryonic growth ($43\%$) and seedling development ($32\%$). However, the supplementation of basal media with kinetin, thidiazuron, 6-benzyl aminopurine or $GA_3$ had no effect on the germination of the embryos. The embryos derived from immature seeds germinated but the frequency of embryonic growth was better in mature seeds. Stratification of seeds effected precocious germination of embryos. Seeds kept at $4^{\circ}C$ for 1 week germinated earlier and at a higher frequency irrespective of the stage of seed maturity, while the germination rate declined with prolonged cold treatment for 1 month at that same temperature. Analysis of taxanes in germinating seedlings revealed that root tissues contained high levels of taxol, 10-deacetyl-baccatin ill and baccatin ill as compared to shoots. Thus embryo culture technique appears to overcome the lengthy dormancy requirement of T. wallichiana seeds.

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문제 정의

wallichiana. The objective of the present study was to develop a protocol for in vitro propagation by embryo culture technique.

제안 방법

, 1998). Quantitative analysis of taxol and related taxanes were performed in a Shimadzu liquid chromatograph (LC 10AD) employing a reverse phase ODS-Hypersil C-18 column (150 mm x 4.6 mm id), a Supelco guard column (Pelliguard^TMLC-18 kit with 2 cm x 4.6 mm cartridge) and isocratic eultion with acetonitrile: water: methanol (30:30:40). The flow rate was 1 ml/min and all chromatographs were plotted at 227nm, using a UV detector as published earlier (Miller et al.

대상 데이터

Each treatment is based on four replicates, each of which consisted of 10 explants.
Seeds of T. wallichiana were collected in October-November 2000-2001 from trees of natural habitat growing at Rimbik (altitude 2313 m), Darjeeling district of West Bengal. Materials were used for in vitro culture immediately after collection or upon arrival, except for some experiments, where the seeds were subjected to refrigeration at 4 ℃ for various periods.

데이터처리

Comparison of the mean values ± S.E. obtained in treatments at respective stages was made using ANOVA and Duncan's multiple range test at 0.05 level of confidence. For each treatment, mean values followed by same letters are not significantly different from each other.
5 cms noted at the end of the 6th week. The experimental data were statistically analyzed with Analysis of Variance (ANOVA); compared at p<0.05 and Duncan's multiple range test (Gomez and Gomez, 1984). The mean of the treatments has been calculated with standard error.

성능/효과

3 cms in length even after 12 weeks of culture. On examining the effect of basal media on seedling development, it was revealed that 32% seedlings germinated in 1/2 WPMSH basal medium supplemented with 1% AC in contrast to 9% germination of the embryos in % MS basal medium (Table 2, Figure 3). The present study also indicates that while 1/2 WPMSH basal medium induced 29% of the embryos to develop into hjll seedlings, about 21% of the embryos developed into full seedings when it was cultured on B5 basal medium.

참고문헌 (32)

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