[논문]Production of Virus Free Seeds using Meristem Culture in Tomato Plant under Tropical Conditions

Alam M.F.; Banu M.L.A.; Swaraz A.M.; Parvez S.; Hossain M.; Khalekuzzaman M.; Ahsan N.

Production of Virus Free Seeds using Meristem Culture in Tomato Plant under Tropical Conditions 원문보기

Journal of plant biotechnology, v.6 no.4, 2004년, pp.221 - 227

Alam M.F. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Banu M.L.A. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Swaraz A.M. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Parvez S. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Hossain M. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Khalekuzzaman M. (Plant Biotechnology Lab. Department of Botany, University of Rajshahi) , Ahsan N. (Department of Animal Science and Biotechnology. Kyungpook National University)

Abstract ▼ AI-Helper

Protocol was established for production of virus free healthy seeds using meristem ($0.3-0.5\;\cal{mm}$ in size) culture and field management under net house condition in tomato. The isolated meristem was found well established in MS liquid medium containing $0.1\;\cal{mg}\;1^{-1}\;of\;GA_3$. For shoot and root development either from primary meristem or from nodal segment of meristem derived plants, semisolid MS medium having $0.5\;\cal{mg}\;1^{-1}$ of IBA was found most effective. The elimination of the studied viruses (ToMV, CMV, ToLCV) in meristem-derived plants was confirmed by DAS-ELISA test. For field management of the virus eradicated meristem-derived plants, use of net house was found very effective measures to check viral vector visit and eventually infection. The meristem-derived plants were vigor and high yielder than the native seed derived plants and produced healthy seeds. Due to stop vector visit, no viral symptoms were observed in both $R_1\;and\;R_2$ plants cultivated in net house condition. Starting of viral infestation was observed in $R_2$ generation when they were planted in open house condition without control of vector visit. Therefore, for management of viral diseases, use of virus free meristem derived plantlets and their subsequent cultivation in soil under net house condition without using any vector killing insecticide can be recommended for producing healthy seeds in tomato. The developed protocol for environmentally healthy tomato seed production in Bangladesh may be used in the countries having similar tropical like environment conducive for viral vector visit.

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가설 설정

B, Developed (14 days old) isolated meristem in liquid medium. C, Primary developed meristem sub-cultured on semi solid medium. D, Rooting of meristem-derived plantlet.

제안 방법

For this purpose the experiment was conducted in two steps. In the first step, the experiment was done to examine the use of meristem culture for producing vhus free clones, and in the second step, management of virus free meristem derived plants in net house condition for commercial production of healthy seeds.
The entire protocol was in two steps: production of virus free clones, and management of the virus free plants in field. The first step of protocol was, production of high frequent virus free tomato clone using meristem culture, and their subsequent acclimatization in the soil. Meristem with 0.

이론/모형

uere it is mentioned that in every steps of evaluation control plants (native seed derived seedlings) were also planted as check, and no insecticides was used for controlling visit of virus spreading vectors under open field condition. As experimental design "split - plot design" was used, and was replicated three times. Here field condition NC = Not conducted (open field and net house) was considered as main plot and cultivar was considered as subplot.
Table 3. Detection of virus elimination (%) from meristem derived plantlets by DAS- ELISA method. In virus negative plant no colour was detected.

성능/효과

5 mg L^-1) was found most effective for proper shoot and root development. The results of other growth reguators show thatGA₃ (0.5 mg L^-1)was found effective for root induction but the shoot length and root number was not induced enough, where as, GA₃ (1.0 mg L^-1) resulted short shoot development without root induction.
The results of this study provide that the use of meristem for plant regeneration and their subsequent maintenance under net house condition was found very effective method for viral disease eradication management in tomato seed. In addition to this no apparent somaclonal variation resulted from the tissue culture process.
2003). The results on shoot and root development from primary established meristems using GA₃, I BA and Kin showed, use of growth regulators was also needed for rapid shoot and root induction and their further proliferation. Use of auxin including IBA for shoot and root development from isolated meristems was reported in several plants (Langhe and Bruijne 1993; Morris et al.

참고문헌 (35)

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Production of Virus Free Seeds using Meristem Culture in Tomato Plant under Tropical Conditions 원문보기

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Production of Virus Free Seeds using Meristem Culture in Tomato Plant under Tropical Conditions 원문보기

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