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Stimulation of In Vitro Bulblet Growth by the Addition of Liquid Medium in Lilium Oriental Hybrid 'Casablanca' 원문보기

Journal of plant biotechnology, v.6 no.4, 2004년, pp.241 - 246  

Han Bong-Hee (Horticulture Research Institute) ,  Suh Eun-Jung (Horticulture Research Institute) ,  Choi Sung-Lyeol (Horticulture Research Institute) ,  Yae Byeoung-Woo (Research Coordination Division, Rural development Administration) ,  Yu Hee-Ju (Horticulture Research Institute) ,  Goo Dae-Hoe (Horticulture Research Institute)

Abstract ▼ AI-Helper 아이콘AI-Helper

The bulb scales and shoot sections ($7\;\cal{mm}\;\times\;15\;\cal{mm}$) of Lilium oriental hybrid 'Casablanca' were cultured to compare bulblet growth in vitro. Shoots were induced from in vitro grown bulbscales on MS medium with $1.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA, an...

주제어

#Activated charcoal   #shoot formation   #sucrose  

AI 본문요약
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제안 방법

  • This medium was poured in each vessel aseptically. The effects of sucrose concentrations (30 ~ 150 g/L), the strength of MS salts, (1/2 ~ 2 x), and differs  MS salts (MS macro-, MS macro- and micro-, and MS full salts) were examined on bulblet growth for two months after adding the liquid medium. The quantity of liquid medium was also tested.

대상 데이터

  • All experiments were performed in polyester vessels (W 10 cm x 5 cm), and each vesesel contained 100 ml medium. Media were adjusted to 5.
  • For each treatment, 7 explants were cultured on pholy- ester vessels (10 cm diameter x 5 cm height) and three vessels were used for each treatment. Each vessel was regarded as individual replicate and used for statistical analysis.

데이터처리

  • For all experiments, data were collected on number of bulblets and bulblet size two months after the addition of liquid medium. For statistical analysis, means were subjected to Duncan's multiple range test using SAS statistical package (SAS Institute Inc. 1985).
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참고문헌 (19)

  1. De Riek J, Piqueras A, Debergh PC (1977) Sucrose uptake and metabolism in a double layer system for micropropagation of Rosa multiflora. Plant Cell Tiss Org Cult 47: 269-278 

  2. Gerrits ML, Miller W, Kipnis HL, Kolloffel C, Cross T, De Klerk G.J (1997) Bulb growth in lily regenerated in vitro. Acta Hort 430:267-273 

  3. Han BH, Yae BW, Goo DH, Ko JY (1999a) Effects of growth regulators and light on the formation and proliferation of bulblets with swollen basal plate from in vitro culture of bulbscales in Lilium oriental hybrid 'Casablanca'. J Kor Soc Hort Sci 40:463-466 

  4. Han BH, Yae BW, Goo DH, Ko JY (1999b) The formation and growth of bulblets from bulblet sections with swollen basal plate in Lilium oriental hybrid 'Casablanca'. J Kor Soc Hort Sci 40:747-750 

  5. Han BH, Yae BW, Goo DH, Ko JY (1999c) Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulb scales in Lilium oriental hybrid 'Casablanca'. Kor J Plant Tiss Cult 26:103-107 

  6. Lian ML, Chakrabarty D, Peak KY (2002) Growth and uptake of sucrose and mineral by bulblets of Lilium oriental hybrid 'Casablanca' during bioreactor culture. J Hort Sci Biotech 77:253-257 

  7. Maene L, Debergh P (1985) Liquid medium additions to established tissue cultures to improve elongation and rooting in vitro. Plant Cell Tiss Org Cult 5:23-33 

  8. Maesato K, Sharada K, Fukui H, Hara T, Sarma KS (1994) In vitro bulblet regulation from bulbscale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment. J Hort Sci 69:289-297 

  9. Matsuo E, Ohkurano T, Arisumi K, Sakata T (1986) Scale bulblet malformations seen in Lilium longiflorum during scale propagation. HortSci 21:150 

  10. Marinangeli P, Curvetto N (1998) Growth in soil of micro- and macro-propagated Lilium bulblets. Phyton-International J Expt Bot 63:237-244 

  11. Marinangeli P, Curvetto N (1999) In vitro and ex vitro growth of micropropagated Lilium longiflorum Thunb. bulblets. Phyton-International J of experimental Bot 64:133-139 

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15:473-497 

  13. Nhut TN, Le BV, Fukai S, Tanaka M, Van KT (2001) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regul 33:59-65 

  14. Niimi Y (1984) Effect of Q' -naphthaleneacetic acid and 6-benzylaminopurineon the development of excised-bulbs (Lilium rubel/um Baker) cultured in vitro both in diffused light and in continuous darkness, and the leaf emergence from the bulbs in vitro. J Japan Soc Hort Sci 53:59-65 

  15. Stimart DP, Ascher PO (1978) Tissue culture of bulb scale sections for asexual propagation of Lilium longiflorum Thunb. J Amer Soc Hor Sci 103:182-184 

  16. Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiol Plant 46:184-190 

  17. Takayama S, Misawa M (1980) Differentiation in Lilium in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiol Plant 48:121-125 

  18. Van Aartrijk J, Blom-Barnhoorn G.J (1980) Effects of sucrose, mineral salts, and some organic substances on the additious regeneration in vitro of plantlets from bulb-scale tissue of Lilium speciosum . Rubrum'. Acta Hort 109:297-302 

  19. Yae BW, Han BH, Goo DH (2001) Dormancy breaking and in vitro growth of in vitro bulblets in Liliun oriental hybrid 'Casablanca' . J Kor Soc Hort. Sci 42:99-102 

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