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Plant Regeneration Depending on Explant Type in Chrysanthemum coronarium L. 원문보기

Journal of plant biotechnology, v.6 no.4, 2004년, pp.253 - 258  

Chae Won-Byoung (Institute of Life Science and Resources, KyungHee University) ,  Choi Geun-Won (Institute of Life Science and Resources, KyungHee University) ,  Chung In-Sik (Institute of Life Science and Resources, KyungHee University)

Abstract ▼ AI-Helper 아이콘AI-Helper

Plant regeneration depending on explant type was inves-tigated with cotyledon, hypocotyl, and leaf explants of garland chrysanthemum (Chrysanthemum coronarium L.) cultured on MS basal medium supplemented with various concentrations of SAP and NAA combination. Among the three different types of expla...

주제어

#Explant type   #garland chrysanthemum   #plant growth regulator   #plant regeneration  

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제안 방법

  • Cotyledon and hypocotyl explants were excised from 1 week-old seedlings and leaf explants were excised from 4 week-old seedlings. 11 to 15 explants per treatment were used and three independent experiments were conducted. Data were collected after 10 weeks of culture.
  • Besides, genetic transformation system to introduce foreign genes into garland chrysanthemum is yet to be standardized. Accordingly, as a part of effort to develop an efficient transformation system, this study was carried out to improve the tissue culture system in garland chrysanthemum by determining proper explant types and optimum culture medium compositions for shoot and root regeneration, and examining the effect of AgNO3 on shoot regeneration.
  • To evaluate the effect of NAA on root formation in addition to sumedia containing 3% sucrose with 0, 0.5, 1.0, and 2.5 μM NAA were tested. As shown on Figure 1B, more vigorous shoot and root growth was observed from the rooting medium containing only 3% sucrose.
  • To investigate the effect of sucrose on rooting, regenerated shoots from hypocotyl explants were transferred to a Mazenta box containing MS medium supplemented with 0, 1, 3, and 5% sucrose. The optimum sucrose concentration determined was applied to examine the effect of NAA (0, 0.
  • To investigate the influence of AgNO3 on shoot regeneration, leaf explants were placed on 95 mm x 20 mm Petri dish containing MS basal medium supplemented with 2.5 μM BAP and NAA, respectively, and 0, 6, 18, and 30 μM AgNO3 and hypocotyl explants were placed on 95 mm x 20 mm Petri dish containing MS medium supplemented with 1.0 BAP and NAA, respectively, and 0, 6, 18, and 30 μM AgNO3. AgNO3 was filter-sterilized and added to the autoclaved medium.

데이터처리

  • Shoot regeneration data were collected twice after 6 and 10 weeks of culture and analyzed with Duncan's multiple range test. For the root regeneration data, the number of roots was counted after 5 weeks of culture and statistically analyzed using standard error.
  • The number of adventitious shoots longer than 3 mm was counted. Shoot regeneration data were collected twice after 6 and 10 weeks of culture and analyzed with Duncan's multiple range test. For the root regeneration data, the number of roots was counted after 5 weeks of culture and statistically analyzed using standard error.
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참고문헌 (20)

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  3. Bais HP, Sudha G, Ravishankar GA (2001) Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cicnorium intybus and their regenerants. Plant Cell Rep 20: 547-555 

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  8. Kaul V, Miller RM, Hutchinson JF, Richards D (1990) Shoot regeneration from stem and leaf explants of Dendranthemagrandiflora Tzvelev (syn. Chrysanthemum morifolium Ramal.). Plant Cell Tiss Org Cult 21: 21-30 

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  16. Sauvadet MA, Brochard P, Boccou-Gibod J (1990) A protoplast-to-plant system in chrysanthemum: differential responses among several commercial clones. Plant Cell Rep 8: 692-695 

  17. Scholl RL, Keathyley DE, Baribault TJ (1981) Enhancement of root formation and fertility in shoots regenerated from antherand seedling-derived callus culture of Arabidopsis thaliana. Z PfIanzenphysiol 104: 225-231 

  18. Sherman JM, Moyer JW, and Daub ME (1998) A regeneration and Agrobacterium-mediated transformation system for genetically diverse Chrysanthemum cultivars. J Amer Soc Hort Sci 123: 189-194 

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